RESUMO
Salting pretreatment is an effective method to improve the quality of frozen fish. This study investigated the quality changes and proteomic profile differences of frozen yellowfin tuna fillets pretreated with ultrasound-assisted salting (UAS) and static salting (SS). This study was centered on three aspects: physicochemical indicators' determination, histological observation, and proteomic analysis. The results showed that UAS significantly increased yield, salt content, and water-holding capacity (WHC), decreased total volatile base nitrogen (TVBN) compared to SS (p < 0.05), and significantly increased water in the protein matrix within myofibrils. Histological observations showed that the tissue cells in the UAS group were less affected by frozen damage, with a more swollen structure and rougher surface of myofibrils observed. Furthermore, 4D label-free proteomics revealed 56 differentially abundant proteins (DAPs) in UAS vs. NT comparison, mainly structural proteins, metabolic enzymes, proteasomes, and their subunits, which are associated with metabolic pathways such as calcium signaling pathway, gap junction, actin cytoskeletal regulation, and necroptosis, which are intimately associated with quality changes in freeze-stored tuna fillets. In brief, UAS enhances the potential for the application of salting pretreatment to improve frozen meat quality, and 4D label-free proteomics provides knowledge to reveal the potential links between quality and molecular changes in processed frozen meat to optimize future UAS meat processing.
RESUMO
The identification of damage based on Lamb waves can hardly avoid obtaining the reference signal under healthy conditions. A non-reference damage localization (NRDL) method is proposed in this paper. The NRDL method is established by the improved two-arrival-time difference method (2/ATDM) and BFGS method. The layout principles of the piezoelectric ceramic lead zirconate titanate (PZT) transducer array in the specimen are proposed. In contrast to existing methods, the damage outside the array in the specimen is identified based on the NRDL. The full-area damage location in the specimen is realized. Furthermore, the optimization of the excitation signal center frequency and transducer array layout is carried out. The damage location accuracy is greatly improved.
Assuntos
Cerâmica , TransdutoresRESUMO
The purpose of this study was to compare minimally invasive and conventional dynamic hip screw techniques for the treatment of intertrochanteric fractures in older patients. Relevant randomized, controlled studies were included. The methodological quality was assessed, and data were extracted independently. Five studies (353 fractures) that compared minimally invasive and conventional dynamic hip screw techniques were included. The rates of serious postoperative complications, operative time (minutes), hemoglobin decrease (g/dL), postoperative Harris Hip Scores, and length of stay (days) were the outcomes of interest. A lower rate of serious postoperative complications was correlated with the minimally invasive dynamic hip screw group compared with the conventional dynamic hip screw group (relative risk, 0.35; 95% confidence interval [CI], 0.16, 0.78), average operative time (weighted mean difference, -16.32; 95% CI, -28.78 to -3.86), hemoglobin decrease (weighted mean difference, -1.44; 95% CI, -1.98 to -0.89), and length of stay (weighted mean difference, -3.72; 95% CI, -5.44 to -2.01) were lower in the minimally invasive dynamic hip screw group, and the postoperative Harris Hip Score (weighted mean difference, 1.42; 95% CI, 0.23 to 2.60) was higher in the minimally invasive dynamic hip screw group.
