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1.
Polymers (Basel) ; 15(4)2023 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-36850127

RESUMO

To accelerate the industrialization of bicomponent fibers, fiber-based flexible devices, and other technical fibers and to protect the property rights of inventors, it is necessary to develop fast, economical, and easy-to-test methods to provide some guidance for formulating relevant testing standards. A quantitative method based on cross-sectional in-situ observation and image processing was developed in this study. First, the cross-sections of the fibers were rapidly prepared by the non-embedding method. Then, transmission and reflection metallographic microscopes were used for in-situ observation and to capture the cross-section images of fibers. This in-situ observation allows for the rapid identification of the type and spatial distribution structure of the bicomponent fiber. Finally, the mass percentage content of each component was calculated rapidly by AI software according to its density, cross-section area, and total test samples of each component. By comparing the ultra-depth of field microscope, differential scanning calorimetry (DSC), and chemical dissolution method, the quantitative analysis was fast, accurate, economical, simple to operate, energy-saving, and environmentally friendly. This method will be widely used in the intelligent qualitative identification and quantitative analysis of bicomponent fibers, fiber-based flexible devices, and blended textiles.

2.
Polymers (Basel) ; 14(20)2022 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-36297932

RESUMO

Organic perovskites are promising optoelectronic semiconductor materials with photoelectric applications. It is known that the luminescence of perovskites is highly sensitive to hydron molecules due to its low moisture resistance of crystal structure, indicating its potential application on humidity-sensing. Herein, a novel perovskite-based compound (PBC) with minimal defects was developed to promote the photoluminescence performance via optimization of the drying method and precursor constitutions. Perovskite materials with good structural integrity and enhanced fluorescence performance up to four times were obtained from supercritical drying. Moreover, the hydrophilic polymer matrix, polyethylene oxide (PEO), was added to obtain a composite of perovskite/PEO (PPC), introducing enhanced humidity sensitivity and solution processibility. These perovskite/PEO composites also exhibited long-term stability and manifold cycles of sensitivity to humidity owing to perovskite encapsulation by PEO. In addition, this precursor solution of perovskite-based composites could be fancily processed by multiple methods, including printing and handwriting, which demonstrates the potential and broaden the applications in architecture decoration, logos, trademarks, and double encryption of anti-fake combined with humidity.

3.
Nano Lett ; 22(9): 3516-3524, 2022 05 11.
Artigo em Inglês | MEDLINE | ID: mdl-35363493

RESUMO

Thermal insulating fibers can effectively regulate the human body temperature and decrease indoor energy consumption. However, designing super thermal insulating fibers integrating a sponge and aerogel structure based on biomass resources is still a challenge. Herein, a flow-assisted dynamic dual-cross-linking strategy is developed to realize the steady fabrication of regenerated all-cellulose graded sponge-aerogel fibers (CGFs) in a microfluidic chip. The chemically cross-linked cellulose solution is used as the core flow, which is passed through two sheath flow channels, containing either a diffusion solvent or a physical cross-linking solvent, resulting in CGFs with a porous sponge outer layer and a dense aerogel inner layer. By regulating and simulating the flow process in the microfluidic chip, CGFs with adjustable sponge thicknesses, excellent toughness (26.20 MJ m-3), and ultralow thermal conductivity (0.023 W m-1 K-1) are fabricated. This work provides a new method for fabricating graded biomass fibers and inspires attractive applications for thermal insulation in textiles.


Assuntos
Celulose , Nanoestruturas , Celulose/química , Humanos , Porosidade , Solventes , Condutividade Térmica
4.
ACS Omega ; 5(33): 21015-21023, 2020 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-32875238

RESUMO

Chronic wounds caused by diabetic or venous diseases remain a social and healthcare burden. In this work, a new strategy is proposed in which injectable thermosensitive chitosan/collagen/ß-glycerophosphate (ß-GP) hydrogels were combined with three-dimensional mesenchymal stem cell (3D MSC) spheroids to accelerate chronic wound healing by enhanced vascularization and paracrine effects. Chitosan/collagen/ß-GP solution mixed with 3D MSC spheroids was rapidly transformed to a gel at body temperature by physical cross-linking, then overlapped the wounds fully and fitted to any shape of the wound. The results showed that the combination therapy exhibited a markedly therapeutic effect than the hydrogel-loaded two-dimensional (2D) MSCs or 2D MSCs alone. The hydrogel could provide an environment conductive to the attachment and proliferation of encapsulated MSCs, especially accelerating the proliferation and paracrine factor secretion of 3D MSC spheroids. These results supplied a novel alternative approach to treat chronic wounds caused by diabetic or venous diseases.

5.
Adv Mater ; 32(10): e1907495, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31984556

RESUMO

Due to the intrinsic properties of fabrics, fabric-based wearable systems have certain advantages over elastomeric material-based stretchable electronics. Here, a method to produce highly stretchable, conductive, washable, and solderable fibers that consist of elastic polyurethane (PU) fibers and conductive Cu fibers, which are used as interconnects for wearable electronics, is reported. The 3D helical shape results from stress relaxation of the prestretched PU fiber and the plasticity of the Cu fiber, which provides a predictable way to manipulate the morphology of the 3D fibers. The present fibers have superior mechanical and electrical properties to many other conductive fibers fabricated through different approaches. The 3D helical fibers can be readily integrated with fabrics and other functional components to build fabric-based wearable systems.


Assuntos
Cobre/química , Poliuretanos/química , Dispositivos Eletrônicos Vestíveis , Elasticidade , Condutividade Elétrica , Têxteis/análise
6.
ACS Omega ; 4(25): 21361-21369, 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31867531

RESUMO

Poly(vinylamine) (PVAm) is an important polymer with the highest content of primary amine groups of any polymer. PVAm has a great potential in selective separation and smart materials. It is difficult to fabricate pure PVAm nanofibers by electrospinning and rotary jet spinning (RJS) without additional polymers. In this work, rotary jet wet spinning (RJWS) was applied to fabricate molecular imprinting nanofibers (MINFs) with polyelectrolyte for the first time. Initially, optimal parameters of spinning are investigated, including coagulation bath, solution viscosity, and rotation speed. The PVAm aqueous solution is sensitive to alcohol. To demonstrate RJWS application, PVAm-based MINFs for bisphenol A (one endocrine disruptor) recognition are prepared by adding dummy template, cross-linking, and template elution. The association constant (8.6 mg/L), equilibrium time (30 min), and binding sites utilization rate (80%) of MINFs are evaluated. Its adsorption amount and selectivity are little lower than those of MIPs prepared by bulk polymerization; however, its adsorption speed is faster than that of MIPs.

7.
Ecotoxicol Environ Saf ; 167: 29-35, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30292973

RESUMO

Perfluorooctanoic acid (PFOA) is a perfluorinated compound that is widely distributed, is persistent in the environment, and has a low-level chronic exposure effect on human health. The aim of this study was to investigate the peroxisome proliferator activated receptors γ (PPARγ) and the sterol regulatory element-binding protein 2 (SREBP2) signaling pathways in regulating the lipid damage response to PFOA in the livers of amphibians. Male and female frogs (Rana nigromaculata) were exposed to 0, 0.01, 0.1, 0.5 and 1 mg/L PFOA. After treatment, we evaluated the pathological changes in the liver by Oil Red O, staining and examined the total cholesterol (T-CHO) and triglyceride (TG) contents. The mRNA expression levels of PPARγ, Fatty acid synthase (FAS), Acetyl-CoA carboxylase (ACC), Glycerol-3-phosphate acyltransferase (GPAT), SREBP2 and 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The administration of PFOA caused marked lipid accumulation damage in the amphibian livers. The T-CHO contents were elevated significantly after PFOA treatment; these results show a dose-dependent manner in both sexes. The TG content showed a significant increase in male livers, while it was elevated significantly in female livers. The RT-PCR results showed that the mRNA expression levels of PPARγ, ACC, FAS, GPAT, SREBP2 and HMG-CoA were significantly dose-dependently increased in the PFOA-treated groups compared with those of the control group. Our results demonstrated that PFOA-induced lipid accumulation also affected the expression levels of genes FAS, ACC, GPAT and HMG-CoA in the PPARγ and SREBP2 signaling pathways in the liver. These finding will provide a scientific theoretical basis for the protection of Rana nigromaculata against PFOA effects.


Assuntos
Caprilatos/toxicidade , Fluorocarbonos/toxicidade , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Ranidae/metabolismo , Acil Coenzima A/metabolismo , Animais , Colesterol/metabolismo , Relação Dose-Resposta a Droga , Ácido Graxo Sintases/genética , Ácido Graxo Sintases/metabolismo , Feminino , Fígado/metabolismo , Masculino , PPAR gama/genética , PPAR gama/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteína de Ligação a Elemento Regulador de Esterol 2/genética , Proteína de Ligação a Elemento Regulador de Esterol 2/metabolismo , Triglicerídeos/metabolismo
8.
Environ Pollut ; 238: 1035-1043, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29459119

RESUMO

Perfluorooctanoic acid (PFOA) is widely distributed in various environmental media and is toxic to organisms. This study demonstrated that PFOA induces hepatotoxicity in the frog and evaluated the role of CYP3A and the Nrf2-ARE signaling pathway in regulating responses to PFOA-induced hepatotoxicity. Rana nigromaculata were exposed to 0, 0.01, 0.1, 0.5, or 1 mg/L PFOA solutions in a static-renewal system for 14 days. Liver tissue samples were collected 24 h after the last treatment. Hepatic histology was observed by HE staining and transmission electron microscopy. The oxidative stress levels in the liver were measured. The expression levels of CYP3A, Nrf2, NQO1, and HO-1 mRNA were measured by quantitative reverse transcription-polymerase chain reaction. PFOA-treated frog liver tissue exhibited diffuse cell borders, cytoplasmic vacuolization, broken nuclei, nuclear chromatin margination, and swollen mitochondria. In addition, the livers of PFOA-treated frogs showed a significantly elevated content of reactive oxygen species, malondialdehyde, glutathione and glutathione S-transferase activity compared to the livers of control frogs. However, the glutathione peroxidase activities concomitantly decreased in PFOA-treated frogs compared to those in the control group. Furthermore, compared with control frogs, the expression levels of CYP3A, Nrf2, and NQO1 mRNA significantly increased in PFOA-treated frogs. HO-1 mRNA expression remarkably increased only in groups treated with 0.5 or 1 mg/L PFOA. Our results indicate that PFOA induces hepatotoxicity in a dose-dependent manner. Furthermore, the results of the comparison analysis between different gender groups illustrated that PFOA is more toxic to female frogs than male frogs. Our results demonstrated that PFOA causes liver damage and that CYP3A enhances PFOA-induced female frogs hepatotoxicity are more virulent than male through biotransformation, and the activation of the Nrf2-ARE pathway is induced to protect against hepatotoxicity in Rana nigromaculata, all of which provide the scientific basis for the protection of amphibians against environmental contaminants.


Assuntos
Caprilatos/toxicidade , Poluentes Ambientais/toxicidade , Fluorocarbonos/toxicidade , Fator 2 Relacionado a NF-E2/metabolismo , Ranidae/fisiologia , Animais , Doença Hepática Induzida por Substâncias e Drogas/patologia , Glutationa/metabolismo , Fígado/metabolismo , Masculino , Malondialdeído/metabolismo , Oxirredução , Estresse Oxidativo , Substâncias Protetoras/metabolismo , Ranidae/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais
9.
Environ Pollut ; 236: 12-20, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29414332

RESUMO

Microcystin-leucine-arginine (MCLR) is the most popular and toxic variant among microcystins, which can cause severe reproductive toxicity to animals. However, the mechanisms of reproductive toxicity induced by MCLR in amphibians are still not entirely clear. In the current study, toxicity mechanisms of MCLR on the reproductive system of male Rana nigromaculata followed by low concentration (0, 0.1, 1, and 10 µg/L) and short-term (0, 7, and 14 days) MCLR exposure were shown. It was observed that MCLR could be bioaccumulated in the testes of male frogs, and the theoretical bioaccumulation factor values were 0.24 and 0.19 exposed to 1 µg/L and 10 µg/L MCLR for 14 days, respectively. MCLR exposure significantly decreased testosterone (T) concentrations and increased estradiol (E2) concentrations exposed to 1 and 10 µg/L MCLR for 14 days. The mRNA levels of HSD17B3 were downregulated, and HSD17B1 and CYP19A1 mRNA expression levels were upregulated, respectively. Only 10 µg/L MCLR group showed significant induction of follicle-stimulating hormone (FSH) levels and cyclic adenosine monophosphate (cAMP) content. Moreover, AR and ESR1 mRNA expression levels were significantly upregulated exposed to 1 and 10 µg/L MCLR for 14 days, respectively. Our results suggested that low-concentration MCLR induced transcription changes of CYP19A1, HSD17B3, and HSD17B1 led to endocrine disorders, and caused interference of spermatogenesis by the decrease of T and abnormal gene expressions of AR and ESR1 in the testes of R. nigromaculata.


Assuntos
Hormônios Esteroides Gonadais/biossíntese , Microcistinas/toxicidade , Ranidae/fisiologia , Testículo/efeitos dos fármacos , Animais , Regulação para Baixo , Expressão Gênica , Hormônios Esteroides Gonadais/análise , Masculino , Ranidae/genética , Testículo/química , Testículo/metabolismo , Regulação para Cima , Poluentes Químicos da Água/toxicidade
10.
Fish Shellfish Immunol ; 37(2): 278-85, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24594009

RESUMO

Microcystins (MCs) are secondary metabolites produced by cyanobacteria. Oxidative stress is considered the major cytotoxic mechanism of microcystin-LR (MCLR). Quercetin (QE) is a flavonoid that can eliminate reactive oxygen species (ROS) and elicit anti-inflammatory and anti-apoptotic effects. This study determined the regulatory effect of QE on the cytotoxicity and oxidative stress of Carassius auratus lymphocytes induced by 1 µg/L MCLR in vitro after 24 h. MCLR-mediated cytotoxicity and ROS formation in fish lymphocytes were suppressed by QE in a concentration-dependent manner. In addition, QE enhanced the endogenous antioxidant defense system and the Bax/Bcl-2 ratio to protect fish lymphocytes against oxidative stress and apoptosis induced by MCLR. Glutathione levels and catalase activities increased by approximately 3.9- and 2-fold, respectively, in the QE treatment group (1000 µg/L) compared with the MCLR treatment group. The percentage of apoptosis in the only MCLR treatment group was 59% whereas that in the control group was 23%. The percentage of apoptosis in the high-dose QE treatment group (1000 µg/L) was 29%, lower by nearly half compared with the only MCLR treatment group. QE (1000 µg/L) effectively inhibited the expression of caspase-3 protein by nearly 43% compared with the only MCLR treatment group. The results obtained clearly indicate that QE can effectively prevent MCLR-induced immunotoxicity by eliminating oxidative stress and blocking the mitochondrial apoptotic pathway in fish lymphocytes.


Assuntos
Apoptose/efeitos dos fármacos , Carpa Dourada/metabolismo , Linfócitos/efeitos dos fármacos , Microcistinas/toxicidade , Quercetina/farmacologia , Animais , Antioxidantes/metabolismo , Toxinas Marinhas , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Poluentes Químicos da Água/toxicidade
11.
J Hazard Mater ; 252-253: 382-9, 2013 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-23548922

RESUMO

Previous studies have shown that toxins produced by toxic cyanobacterial blooms are hazardous materials. In the present study, 1 µg/L microcystin-LR (MC-LR) was observed to induce apoptosis in the testes of male Rana nigromaculata via the mitochondrial and endoplasmic reticulum (ER) pathways at exposure times ranging from 7 d to 14 d. The results showed that reactive oxygen species production and malondialdehyde content were positively correlated with exposure time. Antioxidant enzyme contents, such as reduced glutathione and glutathione peroxidase rapidly decreased, implying that the defense system of the testes induces oxidative damage. MC-LR significantly stimulated the release of cytochrome c in the testes, thereby improving the protein expressions of Bax and caspases-3, 8, and 9 (p<0.01) and inhibiting the protein expression of Bcl-2 with prolonged exposure (p<0.01). Ultrastructural observations showed distention of the mitochondria and endoplasmic reticulum and deformation of the nucleolus. Moreover, prolonged exposure times strengthened and weakened the relative expression levels of C/EBP homologous protein and GRP78, respectively. These results indicate that MC-LR-induced apoptosis of the testes in male frogs in vivo may occur through the mitochondrial and ER pathways. It also further proves our previous findings that MC-LR can induce toxicity in the male reproductive system of R. nigromaculata in vitro. The findings show that MC-LR is highly hazardous to frogs and that the accepted drinking water limit of 1 µg/L MC-LR exerts significant toxicity to amphibians.


Assuntos
Microcistinas/toxicidade , Ranidae , Testículo/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Apoptose/efeitos dos fármacos , Caspases/metabolismo , Citocromos c/metabolismo , Retículo Endoplasmático , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Proteínas de Choque Térmico/metabolismo , Masculino , Malondialdeído/metabolismo , Toxinas Marinhas , Microscopia Eletrônica de Transmissão , Mitocôndrias , Estresse Oxidativo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Testículo/metabolismo , Testículo/ultraestrutura , Fator de Transcrição CHOP/metabolismo , Proteína X Associada a bcl-2/metabolismo
12.
Fish Shellfish Immunol ; 34(5): 1085-93, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23403155

RESUMO

Nodularin is one of the most conspicuous and widespread pollutants that elicit water ecological hazards to fish, causing serious damage on the immune system and physiological functions. Nodularin can cause oxidative stress-induced apoptosis on fish lymphocytes. The regulatory effects of epigallocatechin-3-gallate (EGCG) at 10, 100, and 1000 µg/L levels on the antioxidant defense system and apoptosis of Carassius auratus lymphocytes exposed to a high dose of nodularin (100 µg/L) were quantified in vitro. EGCG reduced nodularin-induced oxidative damage on fish immune cells. This compound significantly increased the activities of superoxide dismutase and catalase and the level of glutathione but decreased the levels of intracellular reactive oxygen species and malondialdehyde. Flow cytometry results showed that the percentages of apoptotic cells after treatment with 10, 100, and 1000 µg/L EGCG for 12 h reached 27.9%, 19.1%, and 13.7%, respectively. By contrast, the nodularin alone-induced group showed a high percentage of apoptosis (44.2%). Western blot analysis showed the increased expression of bcl-2 and the decreased expression of bax and caspase-3 in EGCG-treated fish lymphocytes. EGCG also inhibited the potential collapse of the mitochondrial membrane. Overall, EGCG can inhibit nodularin-induced apoptosis and protect the normal immunity of fish by regulating bax/bcl-2 and blocking the downstream of mitochondrial apoptosis pathway with increased intracellular antioxidant enzyme activity.


Assuntos
Antioxidantes/metabolismo , Catequina/análogos & derivados , Carpa Dourada/metabolismo , Linfócitos/efeitos dos fármacos , Peptídeos Cíclicos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Apoptose/efeitos dos fármacos , Western Blotting/veterinária , Catequina/metabolismo , Células Cultivadas , Relação Dose-Resposta a Droga , Feminino , Citometria de Fluxo/veterinária , Linfócitos/citologia , Linfócitos/metabolismo , Masculino , Toxinas Marinhas/toxicidade , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Nodularia/química , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo
13.
Aquat Toxicol ; 126: 283-90, 2013 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-23084047

RESUMO

This study aims to demonstrate that microcystin-LR (MC-LR) has toxic effects on the reproductive system of male Rana nigromaculata in vitro. R. nigromaculata were treated with 0, 0.1, 1, 10, and 100 nmol/L of MC-LR for 6 h. Results show that exposure to 1 nmol/L to 100 nmol/L of MC-LR decreased sperm motility and number of sperm cells and increased the sperm abnormality rate, whose values were significantly different from those of the control (P<0.01). Moreover, the same dosage of MC-LR increased reactive oxygen species production and malondialdehyde content. At the same time, antioxidant enzyme (catalase and glutathione S-transferase) activity and glutathione reduced content rapidly increased, whereas antioxidant enzyme superoxide dismutase activity significantly decreased. These results imply that the defense system of the testes quickly responds to oxidative stress. Ultrastructural observation shows distention of the mitochondria, endoplasmic reticulum, and Golgi apparatus and changes in the mitochondrial matrix color, cristae number, and morphology. Moreover, using real-time PCR, increased relative expressions of P450 aromatase and SF-1 genes were observed. The results demonstrate for the first time that MC-LR could induce toxicity in the male reproductive system of R. nigromaculata. The findings in this research will provide more insights into the relationships between aquatic microcystins and amphibians.


Assuntos
Genitália Masculina/efeitos dos fármacos , Microcistinas/toxicidade , Ranidae , Poluentes Químicos da Água/toxicidade , Animais , Ativação Enzimática/efeitos dos fármacos , Enzimas/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Masculino , Malondialdeído/metabolismo , Toxinas Marinhas , Microscopia Eletrônica de Transmissão , Espécies Reativas de Oxigênio/metabolismo , Células de Sertoli/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos
14.
Fish Shellfish Immunol ; 33(6): 1229-37, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22951228

RESUMO

Nodularin, a metabolite of Nodularin spumigena, is widely detected in water blooms worldwide and causes serious negative effects on fish. The apoptosis-related cytotoxic effects and mechanisms of nodularin on Carassius auratus lymphocytes were investigated. Transmission electron microscopy results showed that nodularin-treated lymphocytes display a series of morphological changes, including condensed cytoplasm, nuclear chromatin agglutination and marginalization. DNA fragmentation was verified by the DNA-ladder and formation of sub-G1 DNA peaks. These cell characteristics confirmed the occurrence of apoptosis in lymphocytes. Flow cytometric results showed that the percentages of apoptotic cells incubated with 1, 5, 10, and 100 µg/L nodularin for 12 h reached 15.76%, 17.36%, 20.34% and 44.21%, respectively; controls showed low rates of apoptosis (2.4%). The mechanism of apoptosis induced by nodularin was determined, and results showed that nodularin exposure caused a significant increase in intracellular reactive oxygen species (ROS), loss of mitochondrial transmembrane potential in a dose-dependent manner, upregulation of intracellular Ca²âº, downregulation of Bcl-2 and upregulation of Bax expression at the mRNA and protein levels, and activation of caspase-3 and caspase-9 without caspase-8. In summary, all the results suggest that nodularin induces lymphocyte apoptosis via the mitochondrial apoptotic pathway and destroys the immune response of fish.


Assuntos
Apoptose/efeitos dos fármacos , Carpa Dourada , Linfócitos/efeitos dos fármacos , Toxinas Marinhas/toxicidade , Nodularia/química , Peptídeos Cíclicos/toxicidade , Animais , Cálcio/metabolismo , Fragmentação do DNA/efeitos dos fármacos , Relação Dose-Resposta a Droga , Citometria de Fluxo/veterinária , Técnicas In Vitro , Linfócitos/ultraestrutura , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Microscopia Eletrônica de Transmissão/veterinária , Espécies Reativas de Oxigênio/metabolismo
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