Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Insect Physiol ; 52(3): 269-81, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16386270

RESUMO

Larvae of endoparasitoids undergo extensive morphological changes and often have special features to allow their development inside the host. We present the first detailed study on the development of the anal vesicle and the gut. The analyses reveal that the anal vesicle is first seen on the dorsal side of the abdomen as an internal structure covered by a membrane. The morphology of the abdomen then changes intensively: new segments are formed and the anal vesicle develops from a crest of large cells to a protrusion. Towards the end of the first instar, the anal vesicle is fully evaginated and no longer covered by a membrane; the large epithelial cells have microvilli on their apical side which suggests uptake of nutrients from the host's haemolymph. When the larva has moulted to the second instar, the ultrastructure of the anal vesicle begins to change and shows signs of degeneration. In this stage the epithelium of the midgut is fully developed and has a brush border which suggests that nutrient uptake occurs now primarily through the midgut. The anal vesicle then degenerates completely. The salivary glands are prominent already in first instar larvae and appear to produce and release a host regulatory 212 kD protein.


Assuntos
Canal Anal/fisiologia , Proteínas de Insetos/fisiologia , Metamorfose Biológica/fisiologia , Glândulas Salivares/fisiologia , Spodoptera/parasitologia , Vespas/fisiologia , Canal Anal/crescimento & desenvolvimento , Canal Anal/ultraestrutura , Animais , Western Blotting , Interações Hospedeiro-Parasita , Imuno-Histoquímica , Larva/crescimento & desenvolvimento , Larva/fisiologia , Larva/ultraestrutura , Microscopia Confocal , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Glândulas Salivares/crescimento & desenvolvimento , Glândulas Salivares/ultraestrutura , Vespas/crescimento & desenvolvimento , Vespas/ultraestrutura
2.
J Insect Physiol ; 51(3): 287-96, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15749111

RESUMO

Chelonus inanitus (Braconidae) is a solitary egg-larval parasitoid which lays its eggs into eggs of Spodoptera littoralis (Noctuidae); the parasitoid larva then develops in the haemocoel of the host larva. Host embryonic development lasts approx. 3.5 days while parasitoid embryonic development lasts approx. 16 h. All stages of host eggs can be successfully parasitized, and we show here that either the parasitoid larva or the wasp assures that the larva eventually is located in the host's haemocoel. (1) When freshly laid eggs, up to almost 1-day-old, are parasitized, the parasitoid hatches while still in the yolk and enters the host either after waiting or immediately through the dorsal opening. (2) When 1-2-day-old eggs are parasitized, the host embryo has accomplished final dorsal closure and is covered by an embryonic cuticle when the parasitoid hatches; in this case the parasitoid larva bores with its moving abdominal tip into the host. (3) When 2.5-3.5-day-old eggs are parasitized, the wasp oviposits directly into the haemocoel of the host embryo; from day 2 to 2.5 the embryo is still very small and the wasps, after probing, often restrain from oviposition for a few hours.


Assuntos
Oviposição/fisiologia , Spodoptera/parasitologia , Vespas/fisiologia , Animais , Interações Hospedeiro-Parasita , Larva/parasitologia , Larva/fisiologia , Larva/ultraestrutura , Microscopia Eletrônica de Varredura , Óvulo/citologia , Óvulo/parasitologia , Spodoptera/embriologia , Spodoptera/ultraestrutura , Vespas/embriologia , Vespas/ultraestrutura
3.
J Gen Virol ; 84(Pt 5): 1141-1150, 2003 May.
Artigo em Inglês | MEDLINE | ID: mdl-12692279

RESUMO

Polydnaviruses are unique symbiotic viruses that are replicated in the calyx cells of the ovary of some parasitic wasps. They have a segmented genome of circular double-stranded DNA and are injected along with the wasp's egg into the host, where they are essential for successful parasitism. Polydnaviruses replicate from integrated proviral DNA, and after excision of viral segments, flanking DNA is rejoined. Little is known about ovarian morphogenesis, the mode of amplification of the viral DNA and the involvement of ecdysteroids. Here we have analysed these parameters in the course of pupal-adult development in the braconid wasp Chelonus inanitus. Immediately after pupation, ovarian cells proliferated and calyx cells began to differentiate; at this stage ecdysteroids, in particular 20-hydroxyecdysone, were highest. Thereafter, calyx cells began to increase in size and DNA content and eventually became gigantic. Amplification of non-viral DNA (actin) and viral DNA in its integrated and excised form and of corresponding rejoined flanking regions was measured by quantitative real-time PCR. In the early phase of calyx cell differentiation, copy numbers of actin and integrated viral DNA increased to a similar extent. This, along with the increase in nuclear volume and DNA content in the absence of extensive cell proliferation, suggested polyploidization of the early stage calyx cells. In the following phase, integrated viral DNA was selectively and intensively amplified and eventually excised and circularized. As copy numbers of excised circular viral DNA and rejoined flanking DNA reached similarly high levels, excised viral DNA appeared not to replicate. After adult eclosion, amplification of viral DNA declined.


Assuntos
DNA Viral/metabolismo , Ecdisteroides/metabolismo , Ovário/crescimento & desenvolvimento , Polydnaviridae/fisiologia , Vespas/virologia , Animais , DNA/metabolismo , Replicação do DNA , Feminino , Amplificação de Genes , Dados de Sequência Molecular , Morfogênese , Ovário/citologia , Ovário/ultraestrutura , Ovário/virologia , Polydnaviridae/genética , Reação em Cadeia da Polimerase , Simbiose , Replicação Viral , Vespas/genética , Vespas/crescimento & desenvolvimento
4.
J Gen Virol ; 84(Pt 5): 1151-1163, 2003 May.
Artigo em Inglês | MEDLINE | ID: mdl-12692280

RESUMO

Polydnaviruses are unique symbiotic viruses that are formed only in calyx cells in the ovary of parasitic wasps in the families Braconidae and Ichneumonidae; accordingly, two genera, Bracovirus and Ichnovirus are recognized. We have presented a detailed ultrastructural analysis of ovary and calyx cell differentiation and virion morphogenesis, together with the first data on virion release in a bracovirus. Differentiation of the ovary into germarium/vitellarium and the calyx region begins immediately after pupation. In the periphery and central part of the calyx, some cells and their nuclei begin to enlarge and the DNA content increases. The calyx cell nuclei then further increase and become highly lobulated, nuclear pores become very abundant and the cytoplasm is rich in ribosomes. This suggests synthesis and import of viral envelope proteins as viral envelopes appear in the nuclei shortly later. The appearance of viral envelopes is accompanied by a swelling of the nucleus and a change in electron density. Thereafter, the calyx cells reach the final stage with a highly swollen nucleus containing virogenic stroma and mature virions with nucleocapsids. Up to this stage, the DNA content of nuclei increases 120-fold and the volume 45-fold. The mature calyx cells are positioned in the vicinity of the oviduct lumen; for release of virions first the nuclear and then the plasma membrane disintegrate. On the border of the oviduct lumen, cells of an epithelial layer become phagocytic and remove debris, leading to a calyx fluid that contains only densely packed virions.


Assuntos
Ovário/citologia , Ovário/crescimento & desenvolvimento , Polydnaviridae/fisiologia , Vírion/metabolismo , Vespas/virologia , Animais , Diferenciação Celular , DNA/metabolismo , DNA Viral/metabolismo , Feminino , Morfogênese , Ovário/ultraestrutura , Ovário/virologia , Polydnaviridae/genética , Simbiose , Vírion/fisiologia , Replicação Viral , Vespas/crescimento & desenvolvimento
5.
Rouxs Arch Dev Biol ; 198(2): 57-64, 1989 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28305873

RESUMO

To elucidate the cellular basis of hemoglobin transition inXenopus laevis the distribution of larval and adult hemoglobins was analyzed by indirect immunofluorescence in the circulating erythrocytes during metamorphosis. In addition, the morphological characteristics as well as the capacity for synthesis of DNA and hemoglobin in the erythrocytes were followed during the same developmental period. Our quantitative analysis on the distribution of larval and adult hemoglobins suggests that they are localized in different cells. Hemoglobin transition, therefore, most likely reflects replacement of the larval erythrocyte population by new cells which are committed to adult globin synthesis. Since hemoglobin transition is not accompanied by an increase in the abundance of immature erythroid cells with active DNA synthesis, we assume that the presumptive adult erythroid cells are released into circulation at a relatively advanced stage of maturation. The decline in the synthesis of DNA and larval hemoglobin further indicates that cessation of cell renewal in the larval erythrocyte population may represent a decisive step in hemoglobin transition.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...