Prevalence, genotypes, and infection risk factors of psittacine beak and feather disease virus and budgerigar fledgling disease virus in captive birds in Hong Kong.

Psittacine beak and feather disease virus (PBFDV) and budgerigar fledgling disease virus (BFDV) are significant avian pathogens that threaten both captive and wild birds, particularly parrots, which are common hosts. This study involved sampling and testing of 516 captive birds from households, pet shops, and an animal clinic in Hong Kong for PBFDV and BFDV. The results showed that PBFDV and BFDV were present in 7.17% and 0.58% of the samples, respectively. These rates were lower than those reported in most parts of Asia. Notably, the infection rates of PBFDV in pet shops were significantly higher compared to other sources, while no BFDV-positive samples were found in pet shops. Most of the positive samples came from parrots, but PBFDV was also detected in two non-parrot species, including Swinhoe's white-eyes (Zosterops simplex), which had not been reported previously. The ability of PBFDV to infect both psittacine and passerine birds is concerning, especially in densely populated urban areas such as Hong Kong, where captive flocks come into close contact with wildlife. Phylogenetic analysis of the Cap and Rep genes of PBFDV revealed that the strains found in Hong Kong were closely related to those in Europe and other parts of Asia, including mainland China, Thailand, Taiwan, and Saudi Arabia. These findings indicate the presence of both viruses among captive birds in Hong Kong. We recommend implementing regular surveillance for both viruses and adopting measures to prevent contact between captive and wild birds, thereby reducing the transmission of introduced diseases to native species.

Ferret Pediatrics.

Ferrets are bred to be pets, utilized for hunting, and as laboratory models. Despite the fact that ferrets in some areas of the world are neutered by the breeder before entering the pet trade, the importance of pediatric management should not be overlooked. Pregnant, whelping, and lactating jills should be closely monitored and kept in a quiet, stress-free environment. Hand-rearing baby kits is very challenging due to their requirement for ferret milk. Minimizing maternal stress and disease can prevent the need to hand rear kits. Infectious diseases in juvenile ferrets include canine distemper virus, rotavirus, coccidiosis, feline panleukopenia virus (experimental only), and Toxoplasma-like disease. All juvenile ferrets should be vaccinated against canine distemper and rabies. Congenital diseases are reported to affect the auditory, ocular, cardiovascular, urogenital, central nervous, and musculoskeletal systems. Early detection of these diseases is important to prevent the progression of curable diseases.

Emerging Zoonotic Diseases in Ferrets.

As veterinarians, we may be the first to diagnose emerging zoonotic diseases in ferrets and may be at increased risk of exposure. Pseudomonas luteola is a bacterial infection that causes respiratory disease, panniculitis, sialadenitis, and abscess formation. Hepatitis E virus can cause subclinical infection, acute hepatitis, and persistent infection. Since the 2013 article discussing the 2009 influenza pandemic affecting ferrets, there has been an additional case of suspected anthroponotic infection in a pet ferret and experimental infection with influenza viruses from humans, cats, and dogs.

Effects of various diets on the calcium and phosphorus composition of mealworms (Tenebrio molitor larvae) and superworms (Zophobas morio larvae).

OBJECTIVE To evaluate whether the nutritive quality of Tenebrio molitor larvae and Zophobas morio larvae, which are commonly cultured as live food sources, is influenced by 4 commercially available diets used as nutritional substrates; identify which diet best improved calcium content of larvae; and identify the feeding time interval that assured the highest calcium intake by larvae. ANIMALS 2,000 Zophobas morio larvae (ie, superworms) and 7,500 Tenebrio molitor larvae (ie, mealworms). PROCEDURES Larvae were placed in control and diet treatment groups for 2-, 7-, and 10-day intervals. Treatment diets were as follows: wheat millings, avian hand feeding formula, organic avian mash diet, and a high-calcium cricket feed. Control groups received water only. After treatment, larvae were flash-frozen live with liquid nitrogen in preparation for complete proximate and mineral analyses. Analyses for the 2-day treatment group were performed in triplicate. RESULTS The nutrient composition of the high-calcium cricket feed groups had significant changes in calcium content, phosphorus content, and metabolizable energy at the 2-day interval, compared with other treatment groups, for both mealworms and superworms. Calcium content and calcium-to-phosphorus ratios for larvae in the high-calcium cricket feed group were the highest among the diet treatments for all treatment intervals and for both larval species. CONCLUSIONS AND CLINICAL RELEVANCE A 2-day interval with the high-calcium cricket feed achieved a larval nutrient composition sufficient to meet National Research Council dietary calcium recommendations for nonlactating rats. Mealworm calcium composition reached 2,420 g/1,000 kcal at 48 hours, and superworm calcium composition reached 2,070g/1,000 kcal at 48 hours. These findings may enable pet owners, veterinarians, insect breeders, and zoo curators to optimize nutritive content of larvae fed to insectivorous animals.

Encephalitozoon cuniculi in pet rabbits: diagnosis and optimal management.

Encephalitozoonosis is a significant microsporidial disease of captive pet rabbits (Oryctolagus cuniculus). This article overviews the life cycle, pathogenesis, and host immune response to the parasite. Clinical presentation, differential diagnoses, antemortem diagnostics, and postmortem diagnosis will be discussed. International seroprevalence data and histologic prevalence estimates in the US are presented. A review of current treatment and control recommendations are discussed based on extensive review of controlled studies, which have found fenbendazole to be effective for limiting spread of the disease.

Imaging findings in 14 domestic ferrets (Mustela putorius furo) with lymphoma.

Lymphoma is the most common malignant neoplasia in domestic ferrets, Mustela putorius furo. However, imaging findings in ferrets with lymphoma have primarily been described in single case reports. The purpose of this retrospective study was to describe imaging findings in a group of ferrets with confirmed lymphoma. Medical records were searched between 2002 and 2012. A total of 14 ferrets were included. Radiographs (n = 12), ultrasound (n = 14), computed tomography (CT; n = 1), and magnetic resonance imaging (MRI; n = 1) images were available for review. Median age at the time of diagnosis was 5.2 years (range 3.25-7.6 years). Clinical signs were predominantly nonspecific (8/14). The time between the first imaging study and lymphoma diagnosis was 1 day or less in most ferrets (12). Imaging lesions were predominantly detected in the abdomen, and most frequently included intra-abdominal lymphadenopathy (12/14), splenomegaly (8/14), and peritoneal effusion (11/14). Lymphadenopathy and mass lesions were typically hypoechoic on ultrasound. Mild peritoneal effusion was the only detected abnormality in two ferrets. Mild pleural effusion was the most common thoracic abnormality (3/12). Expansile lytic lesions were present in the vertebrae of two ferrets with T3-L3 myelopathy and the femur in a ferret with lameness. Hyperattenuating, enhancing masses with secondary spinal cord compression were associated with vertebral lysis in CT images of one ferret. The MRI study in one ferret with myelopathy was inconclusive. Findings indicated that imaging characteristics of lymphoma in ferrets are similar to those previously reported in dogs, cats, and humans.

Selected emerging diseases in ferrets.

Since their introduction as pets several decades ago, ferrets have become an increasingly popular household pet. Great strides have been made in improving their diet and understanding common diseases (eg, insulinoma, hyperadrenocorticism, lymphoma) that affect them. With the frequency with which these conditions are seen, it sometimes is easy to forget that ferrets can be affected by other diseases. Some of these diseases, such as cryptococcosis, are known, but may be increasing in incidence and range, whereas others, such as hypothyroidism and pure red cell aplasia, may be underrecognized or underreported. This review highlights new and emerging diseases not already well reviewed in the literature.

Diagnosis and treatment of myelo-osteolytic plasmablastic lymphoma of the femur in a domestic ferret.

CASE DESCRIPTION: A 6-year-old 0.82-kg (1.8-lb) spayed female domestic ferret was evaluated because of a 1-month history of decreased activity that had progressively worsened over the past week. The ferret had previously been determined to have adrenocortical disease and was undergoing medical management for the associated clinical signs. CLINICAL FINDINGS: Physical examination revealed lameness of the right hind limb with evidence of pain elicited during palpation of the right femur. Results of a CBC suggested mild anemia, and those of a serum biochemical analysis indicated a high blood glucose concentration. Radiography of the limb revealed extensive lysis of the right femur. Cytologic evaluation of a fine-needle aspirate of the bone lesion revealed a dominant plasma cell component. Plasma cell neoplasia was suspected on the basis of these findings. TREATMENT AND OUTCOME: Radical right hind limb amputation with mid to caudal hemipelvectomy was performed. Histologic evaluation of the lesion allowed a diagnosis of lymphoma with plasmablastic features, and immunohistochemical testing revealed a few CD79alpha-positive neoplastic cells and rare BLA36-positive cells. Adjunctive antineoplastic treatment with systemically administered multidrug chemotherapy was initiated. Six months after surgery, the ferret was reevaluated, and chemotherapy was discontinued when results of clinicopathologic tests, whole body survey radiography, and abdominal ultrasonography suggested no recurrence of the disease. CLINICAL RELEVANCE: The ferret appeared to cope well with radical hind limb amputation, and the chemotherapeutic protocol used was easy to administer. This treatment approach might lead to better owner and patient compliance in other cases of lymphoma in ferrets.

Ovariohysterectomy in ferrets.

Surgical sterilization of ferrets is a relatively common surgical procedure. It is usually done as a method of contraception and to prevent diseases associated with the reproductive system. This column will describe the anatomy, principles, possible complications and outcomes of surgical ovariohysterectomy in ferrets.

Diagnostic contrast radiography in fish.

Despite the abundance of fish as research models, diagnostic techniques for fish diseases are relatively scarce. With some accommodations for handling fish, the same diagnostic tools that are frequently used for other animals can be applied in fish. This column describes indications, preparation and techniques for contrast radiography in fish.

Lacrimal cystadenoma in a Chinese box turtle (Cuora flavomarginata).

An adult male Chinese box turtle (Cuora flavomarginata) presented to the Avian and Exotic Pet Service of the Animal Medical Center for periorbital swelling of the right eye. The swelling had failed to respond to nutritional supplementation and parenteral administration of vitamin A. What had initially presented as periorbital swelling developed into a growth ventral to the globe that impeded vision and was frequently traumatized by forelimb movements of the turtle. Twenty-six months after initial presentation, the turtle was anesthetized and the bulk of the mass was surgically removed. Histopathologic examination determined the mass to be a benign lacrimal cystadenoma.