Prognostic Biomarker SLCO4A1 Is Correlated with Tumor Immune Infiltration in Colon Adenocarcinoma.

Background: Solute carrier organic anion transporter family member 4A1 (SLCO4A1), a member of solute carrier organic anion family, is a key gene regulating bile metabolism, organic anion transport, and ABC transport. However, the association of SLCO4A1 with prognosis and tumor immune infiltration in colon adenocarcinoma (COAD) remains indistinct. Methods: Firstly, we explored the expression level of SLCO4A1 in COAD via GEPIA, Oncomine, and UALCAN databases. Secondly, we used the Kaplan-Meier plotter and PrognoScan databases to investigate the effect of SLCO4A1 on prognosis in COAD patients. In addition, the correlation between SLCO4A1 and tumor immune infiltration was studied by using TIMER and TISIDB databases. Results: Our results showed that SLCO4A1 was overexpressed in COAD tissues. At the same time, our study showed that high expression of SLCO4A1 was associated with poor overall survival, disease-free survival, and disease-specific survival in COAD patients. The expression level of SLCO4A1 was negatively linked to the infiltrating levels of B cells, CD8+ T cells, and dendritic cells in COAD. Moreover, the expression of SLCO4A1 was significantly correlated with numerous immune markers in COAD. Conclusions: These results indicated that SLCO4A1 could be associated with the prognosis of COAD patients and the levels of tumor immune infiltration. Our study suggested that SLCO4A1 could be a valuable biomarker for evaluating prognosis and tumor immune infiltration in COAD patients.

Application of Quantitative Assessment Strategy-Based Nursing Combined with Empathic Nursing in Patients Undergoing Tension-Free Inguinal Herniorrhaphy.

Objective: This study aimed to explore the application of quantitative assessment strategy-based nursing combined with empathic nursing in patients undergoing tension-free inguinal herniorrhaphy. Methods: 82 patients who underwent tension-free inguinal herniorrhaphy in our hospital from May 2020 to December 2021 were enrolled. According to the random number table method, they were divided into three groups (A, B, and C). Patients in group A received quantitative assessment strategy-based nursing combined with empathic nursing, and group B received quantitative assessment strategy-based nursing. Group C received a routine intervention. The early recovery of the three groups was observed. Heart rate (HR), oxygen saturation (SpO2), and blood pressure (BP) were recorded before entering the operating room and at the beginning of anesthesia. Mental Health Continuum-Short Form (MHC-SF, adult version) was used to assess the psychological status. The Modified Barthel Index (MBI) score of three groups at different time points was recorded. The complications of the three groups were observed. Results: Compared with group C, wound healing time, ambulation time, hospital stay time, and time to resume daily activities in groups A and B were significantly shorter (all P < 0.05). At the beginning of anesthesia, HR and BP levels in the three groups were all decreased, with HR and BP levels in group A lower than those in group C and HR levels in group B lower than those in group C (all P < 0.05). After the intervention, the scores of emotional well-being, psychological well-being, and social well-being in groups A and B were higher than those in group C and the scores of psychological well-being and social well-being in group A were higher than those in group B (all P < 0.05). The daily living ability score of group A was higher than that of groups B and group C at discharge, one month after discharge, and three months after discharge, and the daily living ability score of group B was higher than that of group C at these three time points (all P < 0.05). No significant differences were observed in the incidence of complication rate among the three groups (10.71%, 14.81%, and 29.63%) (χ 2 = 3.616, all P > 0.05). Conclusion: Quantitative evaluation strategy-based nursing combined with empathic nursing can effectively improve the early recovery of patients undergoing tension-free inguinal herniorrhaphy, reduce their stress response, and improve their mental health and daily living ability.