RESUMO
The colorectal neoplasia differentially expressed (CRNDE) gene encodes a long non-coding RNA (lncRNA) that is the most unregulated among 129 lncRNAs differentially expressed in gliomas. In this study, we confirmed high CRNDE expression in clinical glioma specimens and observed through experiments in human glioma cell lines a novel molecular mechanism by which CRNDE may contribute to glioma pathogenesis. By inducing or silencing CRNDE expression, we detected a positive correlation between CRNDE levels and the proliferative, migratory, and invasive capacities of glioma cells, which were concomitant with a decreased apoptosis rate. Our experiments also suggest that these effects are mediated by downregulation of miR-136-5p, which correlated with the glioma WHO grade. Based on predicted CRNDE/miR-136-5p/mRNA interactions, both the mRNA and protein expression analyses suggested that miR-136-5p-mediated repression of Bcl-2 and Wnt2 underlies the pro-tumoral actions of CRNDE. We therefore propose that CRNDE functions as a competing endogenous RNA (ceRNA) that binds to and negatively regulates miR-136-5p, thereby protecting Bcl-2 and Wnt2 from miR-136-5p-mediated inhibition in glioma.
RESUMO
OBJECTIVE: To investigate the clinical features of pediatric cerebrovascular disorders. METHODS: The clinical data of 204 cases of pediatric cerebrovascular disease, 134 male and 70 female (1.91: 1), aged 12.7 (32 d - 17.9 years), hospitalized in the past 13 years were analyzed retrospectively. The diagnosis of intracranial hemorrhage was mainly based on CT, Digital subtraction angiography was conducted on 130 cases and pathological examination was conducted on 78 cases. RESULTS: Intracranial hemorrhage was seen in 195 cases. The onset of symptoms was acute or subacute in 73.5% of the patients (150/204). The main manifestations included headache (70.6%), vomiting (50%), loss of consciousness (22.5%), convulsion (21.6%), and focal neurological deficits (13.2%). The causes included arteriovenous malformation (42.2%, 86/204), cavernoma (16.2%, 33/204), aneurysm (8.8%, 18/204), and moyamoya disease (5.9%, 12/204), and the cause remained unknown in 43 of the patients (21%). CONCLUSION: The epidemiology of pediatric cerebrovascular diseases has its own specificity. Therefore, it is crucial to deal timely with it according to the characteristics of the cerebrovascular diseases in children's.
