[Application of double skin island free forearm flap in the repair of large perforating defect of palate].

Objective:To explore the value of free forearm flap with double skin island in repairing large perforating defect of palate. Method:The free forearm flap with double skin island was used to repair 6 cases of large perforating palatal defect due to oral malignant tumor. Preoperative Allen test and ultrasound doppler examination were used to judge the forearm vessels. Result:All the free forearm flap with double skin island survived in 6 cases, followed up for 3 months to 24 months, the patients ate normally, swallowing without nasal regurgitation. The patients had mild to moderate nasal sounds, and the patients were satisfied with the effect of operation and the quality of life. Conclusion:The double skin island free forearm flap is a reliable method for repairing large perforating defect of palate, with satisfactory morphological function and good effect.

[Application of digital 3D imaging technique in flap design before maxillofacial soft tissue reconstruction].

Objective:Using digital technique to design the preoperative flap of oral and maxillofacial soft tissue defect, to instruct the operation and to provide the basis for making the individual femoral flap. Method:We chosed 7 patients with oral and maxillofacial malignancies to undergo thin-line CT scans of lower extremities and analyzed the data of CTA examination to measure the diameter, shape and length of vascular pedicle. Then selected the appropriate perforation as the source of blood supply for flap. The location of perforation was marked on the skin to simulate flap removal range.During the surgery according to the preoperative mark, draw lines and mark flap size. After flap preparation is completed, suture the donor area directly and build in negative pressure drainage tube. Result:All reconstruction of the anterolateral femoral artery descending perforator flap can show the skin, muscle, blood vessels and their adjacent relationship, and according to the size of the defect area, using Mimics 17.0 software simulation to cut flap which should be the same as the defect area. The main perforating branches and trunks of seven skin flaps were consistent with pre-operative examination and all skin flaps survived; the wound surface and the donor were all in stage Ⅰ healing. After operation, patients were followed up for 1-6 months and the form, quality and function of skin flaps all recovered well. Conclusion:The location of lateral femoral circumflex artery descending limb and perforating branch were accurately located by digital technique three-dimensional reconstruction technology, showing the spatial relationship among skin, muscle, blood vessels and bones. By combining with Mimics 17.0 software, it can achieve three-dimensional and accurate design of anterolateral thigh flap, reduce the donor injury and improve the successful rate of skin flap.

Necrotizing meningoencephalitis and pneumonitis in a simian immunodeficiency virus-infected rhesus macaque due to Acanthamoeba.

Free-living amoebae of the genus Acanthamoeba can cause a fatal disease of the brain in humans called granulomatous amoebic encephalitis. We present a case of meningoencephalitis and pneumonitis in a simian immunodeficiency virus (SIV)-infected rhesus macaque caused by Acanthamoeba sp. The animal became ill 176 days after intravenous inoculation with SIVmac251 after a short history of weight loss and a sudden onset of hind limb paresis and abnormal head movements. Histopathologic examination of hematoxylin and eosin-stained tissues revealed multifocal to coalescing necrotizing neutrophilic meningoencephalitis and pneumonitis. Immunofluorescence and polymerase chain reaction were used to identify the genus of amoeba as Acanthamoeba. Immunohistochemistry of immune cell markers was used to characterize the animal's immune response to the opportunistic amoebic infection with features of both innate and adaptive cell-mediated immunity. Although not previously reported, the potential transmission to humans, either through environmental contamination or contact with an infected animal, makes this disease a threat to laboratory animal care staff and pathologists.

[The pharmacokinetics of SC1001-sodium and SC1001-aminum in human body].

The pharmacokinetics of SC1001-Sodium and SC1001-Aminum in the human body was studied. After 200 mg SC1001-Sodium or SC1001-Aminum by oral administration, plasma concentration-time data were fitted to curves by means of the nonlinear least-square method, employing the program we ourselves compiled. With F-test, comparing gamma 2(1) -value and AIC-value, the linear one compartment open model was available. The mean pharmacokinetic parameters fitted one by one were as follows with the first value given belonging to SC1001-Sodium and the second to SC1001-Aminum: Ka: 0.7248 and 0.5102 h-1; Ke: 0.0597 and 0.1149 h-1; T1/2, a: 1.1437 and 1.8999 h; T1/2, e: 15.7428 and 6.1497 h; AUC: 600.15 and 28.61 micrograms.h/ml; Tm: 4.4798 and 4.3728h.