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Food Chem ; 272: 670-678, 2019 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-30309597

RESUMO

Eurotium cristatum, a beneficial fungus isolated from Fuzhuan tea, was used to ferment Angelica dahurica for the first time. The antioxidant capacities of the extracts before and after fermentation were compared using ABTS, DPPH and FRAP assays. The results showed that the antioxidant capacities of the extracts acquired using organic solvents were greater after fermentation. Moreover, based on a comparison of the HPLC chromatograms, the chemical composition of Angelica dahurica changed substantially during fermentation. To further understand the changes in its antioxidant constituents, an on-line HPLC-PDA-Triple-TOF-MS/MS-ABTS system was employed. Twelve antioxidants belonging to three different classes were detected and identified, and their antioxidant capacities were preliminarily evaluated. The results indicated that the substances produced during the fermentation of Eurotium cristatum played important roles in enhancing the antioxidant capacity.


Assuntos
Angelica/química , Cromatografia Líquida de Alta Pressão , Eurotium/crescimento & desenvolvimento , Sequestradores de Radicais Livres/análise , Espectrometria de Massas em Tandem , Angelica/crescimento & desenvolvimento , Angelica/metabolismo , Reatores Biológicos , Eurotium/metabolismo , Sequestradores de Radicais Livres/química , Furocumarinas/análise , Furocumarinas/química , Alcaloides Indólicos/análise , Alcaloides Indólicos/química , Sistemas On-Line , Policetídeos/análise , Policetídeos/química , Análise de Componente Principal , Reprodutibilidade dos Testes
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