Chloride Intracellular Channel 1 is a Potential Biomarker for Breast Cancer.

Purpose: Multiple reports have demonstrated that highly expressed chloride intracellular channel 1 (CLIC1) exists in a range of malignant tumors and is involved in proliferation, invasion, and migration of cancer cells. There are few studies on CLIC1 and breast cancer (BC). The purpose of this research was to evaluate the expression level of CLIC1 in BC and its impact on prognosis of BC patients. Patients and Methods: Differences in CLIC1 expression levels in 25 pairs of BC and corresponding paracancerous specimens were tested by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot (WB). Immunohistochemistry (IHC) was performed to discuss the relevance between CLIC1 expression in BC tissue chips and clinicopathological parameters of BC patients. The effect of CLIC1 expression on patient prognosis was evaluated by Kaplan-Meier survival curve and Cox regression analysis. Receiver operating characteristic (ROC) curve assessed the diagnostic performance of CLIC1 for BC. Results: The experimental results of qRT-PCR and WB demonstrated that CLIC1 was highly expressed in BC tissues. IHC results showed that overexpression of CLIC1 was strictly correlated with tumor size, TNM classification, pathological grade, lymph node metastasis and Ki67. Patients with lower CLIC1 expression had longer overall survival (OS) and progression-free survival (PFS). Cox regression analysis and ROC curve confirmed that CLIC1 could independently influence the prognosis of BC patients and might have diagnostic efficiency. Conclusion: Overexpressed CLIC1 is closely related to the progression of BC and the poor prognosis of the patients, suggesting that it may act as a potential biological diagnostic index for BC.

Effects of soil type and rainfall intensity on sheet erosion processes and sediment characteristics along the climatic gradient in central-south China.

Soil erosion poses a major threat to the sustainability of natural ecosystems. The main objective of this study was to investigate the effects of soil type and rainfall intensity on sheet erosion processes (hydrological, erosional processes and sediment characteristics) from temperate to tropical climate. Field plot experiments were conducted under pre-wetted bare fallow condition for five soil types (two Luvisols, an Alisol, an Acrisol and a Ferralsol) with heavy textures (silty clay loam, silty clay and clay) derived separately from loess deposits, quaternary red clays and basalt in central-south China. Rainfall simulations were performed at two rainfall intensities (45 and 90mmh-1) and lasted one hour after runoff generation. Runoff coefficient, sediment concentration, sediment yield rate and sediment effective size distribution were determined at 3-min intervals. Runoff temporal variations were similar at the high rainfall intensity, but exhibited a remarkable difference at the low rainfall intensity among soil types except for tropical Ferralsol. Illite was positively correlated with runoff coefficient (p<0.05). Rainfall intensity significantly contributed to the erosional process (p<0.001). Sediment concentration and yield rate were the smallest for the tropical Ferralsol and sediment concentration was the largest for the temperate Luvisol. The regimes (transport and detachment) limiting erosion varied under the interaction of rainfall characteristics (intensity and duration) and soil types, with amorphous iron oxides and bulk density jointly enhancing soil resistance to erosive forces (Adj-R2>88%, p<0.001). Sediment size was dominated by <0.1mm size fraction for the Luvisols and bimodally distributed with the peaks at <0.1mm and 1-0.5mm size for the other soil types. Exchangeable sodium decreased sediment size while rainfall intensity and clay content increased it (Adj-R2=96%, p<0.01). These results allow to better understand the climate effect on erosion processes at the spatial-temporal scale from the perspective of soil properties.

Variation of Soil Aggregation along the Weathering Gradient: Comparison of Grain Size Distribution under Different Disruptive Forces.

The formation and stabilization of soil aggregates play a key role in soil functions. To date, few studies have been performed on the variation of soil aggregation with increasing soil weathering degree. Here, soil aggregation and its influencing factors along the weathering gradient were investigated. Six typical zonal soils (derived from similar parent materials) were sampled from temperate to tropical regions. Grain size distribution (GSD) in aggregate fragmentation with increasing disruptive forces (air-dried, water dispersion and chemical dispersion) was determined by laser diffraction particle size analyzer. Different forms of sesquioxides were determined by selective chemical extraction and their contributions to soil aggregation were identified by multiple stepwise regression analysis. The high variability of sesquioxides in different forms appeared with increasing free oxide content (Fed and Ald) from the temperate to tropical soils. The transformation of GSD peak to small size varied with increasing disruptive forces (p<0.05). Although in different weathering degrees, zonal soils showed a similar fragmentation process. Aggregate water stability generally increased with increasing soil weathering (p<0.01), with higher stability in eluvium (A) horizon than in illuvium (B) horizon (p<0.01). Crystalline oxides and amorphous iron oxides (Feo), especially (Fed-Feo) contributed to the formation of air-dried macroaggregates and their stability against slaking (R2 = 55%, p<0.01), while fine particles (<50µm) and Feo (excluding the complex form Fep) played a positive role in the formation of water stable aggregates (R2 = 93%, p<0.01). Additionally, water stable aggregates (including stability, size distribution and specific surface area) were closely related with pH, organic matter, cation exchange capacity (CEC), bulk density (BD), and free oxides (including various forms) (p<0.05). The overall results indicate that soil aggregation conforms to aggregate hierarchy theory to some extent along the weathering gradient and different forms of sesquioxides perform their specific roles in the formation and stabilization of different size aggregates.

[Development and test of a wheat chlorophyll, nitrogen and water content meter].

A portable meter was developed which can detect chlorophyll, nitrogen and moisture content of wheat leaf simultaneously, and can supply enough data for guiding fertilization and irrigation. This meter is composed of light path and electronic circuit. And this meter uses 660, 940 and 1450 nm LED together with narrow band filters as the active light source. The hardware circuit consists of micro-controller, LED drive circuit, detector, communication circuit, keyboard and LCD circuit. The meter was tested in the field and performed well with good repeatability and accuracy. The relative errors of chlorophyll and nitrogen test were about 10%, relative error for water content was 4%. The coefficients of variation of the three indices were all below 1.5%. All of these prove that the meter can be applied under the field condition to guide the wheat production.