Enzymolytic soybean meal-impact on growth performance, nutrient digestibility, antioxidative capacity, and intestinal health of weaned piglets.

Enzymolytic soybean meal (ESBM) enriches free amino acids and small peptides, while mitigating anti-nutritional factors. Substituting soybean meal with ESBM enhances animal performance, though optimal piglet dietary supplementation levels vary. The present study aimed to assess the impact of ESBM on the growth performance, nutrient digestibility, antioxidative capacity and intestinal health of weaned piglets. A total of 120 piglets (initial body weight, 7.0 ± 0.4 kg) were randomly allocated into 4 dietary groups, each comprising 5 replicates with 6 piglets per replicate. The control group received the basal diet, while the experimental groups were fed diets containing 2, 4% or 8% ESBM as a replacement for soybean meal over 28 days. Compared with the control group, piglets supplemented with 4% ESBM exhibited a significant increase (p < 0.05) in average daily gain and the apparent total tract digestibility of dry matter, ether extract and gross energy (p < 0.05), alongside a notable decrease (p < 0.05) in diarrhea incidence. Fed ESBM linearly increased (p < 0.05) the villus height in the ileum of piglets. The levels of superoxide dismutase and total antioxidant capacity in serum of piglets increased (p < 0.05) in the 2 and 4% ESBM groups, while diamine oxidase content decreased (p < 0.05) in the 4 and 8% ESBM group. ESBM inclusion also upregulated (p < 0.05) the expression of superoxide dismutase 1 (SOD-1), Catalase (CAT) and claudin-1 mRNA. In terms of cecal fermentation characteristics, ESBM supplementation resulted in a increase (p < 0.05) in valerate content and a linear rise (p < 0.05) in propionate, butyrate, and total short-chain fatty acids levels, accompanied by a decrease (p < 0.05) in the concentrations of tryptamine and NH3 in cecal digesta. ESBM had no discernible effect on cecal microbial composition. In summary, substitution of soybean meal with ESBM effectively improved the growth performance of piglets by enhancing nutrient digestibility, antioxidant capacity, intestinal barrier and cecal microbial fermentation characteristics, with the optimal replacement level identified at 4%.

Benefits of heat-killed Lactobacillus acidophilus on growth performance, nutrient digestibility, antioxidant status, immunity, and cecal microbiota of rabbits.

Introduction: Heat-killed probiotics, as a type of inactivated beneficial microorganisms, possess an extended shelf life and broader adaptability compared to their live counterparts. This study aimed to investigate the impact of heat-killed Lactobacillus acidophilus (L. acidophilus, LA) - a deactivated probiotic on the growth performance, digestibility, antioxidant status, immunity and cecal microbiota of rabbits. Methods: Two hundred weaned Hyla rabbits were randomly allocated into five equal groups (CON, L200, L400, L600, and L800). Over a 28-day period, the rabbits were fed basal diets supplemented with 0, 200, 400, 600, and 800 mg/kg of heat-killed LA, respectively. Results: Results revealed a significant reduction in the feed-to-gain ratio (F/G) in the L600 and L800 groups (p < 0.05). Additionally, the L800 group exhibited significantly higher apparent digestibility of crude fiber (CF) and crude protein (CP) (p < 0.05). Regarding digestive enzyme activities, enhanced trypsin and fibrinase activities were observed in the L600 and L800 groups (p < 0.05). Concerning the regulation of the body's antioxidant status, the L800 group demonstrated elevated levels of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in both serum and ileal tissue (p < 0.05). In terms of immune capacity modulation, serum tumor necrosis factor-α (TNF-α) levels were significantly lower in the L600 and L800 groups (p < 0.05), while immunoglobulin A (IgA) and immunoglobulin M (IgM) levels were higher (p < 0.05). Additionally, the L800 group exhibited a substantial increase in secretory immunoglobulin A (SIgA) levels in the intestinal mucosa (p < 0.05). In comparison to the CON group, the L800 group exhibited a significant increase in the relative abundance of Phascolarctobacterium and Alistipes in the cecum (p < 0.05). Phascolarctobacterium demonstrated a positive correlation with SIgA (p < 0.05), IgM (p < 0.01), and Glutathione peroxidase (GSH-Px) (p < 0.05), while displaying a negative correlation with TNF-α levels (p < 0.05). Concurrently, Alistipes exhibited positive correlations with IgA (p < 0.05), IgM (p < 0.05), SIgA (p < 0.01), GSH-Px (p < 0.05), SOD (p < 0.05), and T-AOC (p < 0.01), and a negative correlation with TNF-α (p < 0.05). Discussion: In conclusion, the dietary incorporation of 600 mg/kg and 800 mg/kg of heat-killed LA positively influenced the growth performance, nutrient digestibility, antioxidant status, immune capacity and cecal microbiota of rabbits. This highlights the potential benefits of utilizing heat-killed probiotics in animal nutrition.

Carvacrol attenuated lipopolysaccharide-induced intestinal injury by down-regulating TLRs gene expression and regulating the gut microbiota in rabbit.

Carvacrol (CAR) is a plant extract that has been reported to enhance antioxidant activity in animals. However, the effect of CAR on the intestinal health of rabbits is poorly understood. Here, we investigated whether CAR exerts protective effects on the intestinal health of rabbits following lipopolysaccharide (LPS) challenge and whether these effects were mediated via the reduction of intestinal inflammation and the regulation of the intestinal flora. Intestinal damage was assessed in LPS-challenged rabbits treated or not with CAR. The serum levels of inflammatory factors were assessed by enzyme-linked immunosorbent assay. Histopathological changes in the ileum and cecum were examined using hematoxylin and eosin staining. The relative gene expression levels of inflammatory factors and tight junction proteins in the rabbit cecum were determined by qRT-PCR. High-throughput sequencing analysis of the microbial 16S rRNA gene was performed using the Illumina NovaSeq Platform. The results showed that CAR can prevent intestinal inflammation and damage as well as mitigate gut dysbiosis in rabbits following LPS challenge. Our study provides a theoretical reference for the application of dietary CAR in rabbit production.

Challenges and advances for glioma therapy based on inorganic nanoparticles.

Glioma is one of the most serious central nervous system diseases, with high mortality and poor prognosis. Despite the continuous development of existing treatment methods, the median survival time of glioma patients is still only 15 months. The main treatment difficulties are the invasive growth of glioma and the obstruction of the blood-brain barrier (BBB) to drugs. With rapid advancements in nanotechnology, inorganic nanoparticles (INPs) have shown favourable application prospects in the diagnosis and treatment of glioma. Due to their extraordinary intrinsic features, INPs can be easily fabricated, while doping with other elements and surface modification by biological ligands can be used to enhance BBB penetration, targeted delivery and biocompatibility. Guided glioma theranostics with INPs can improve and enhance the efficacy of traditional methods such as chemotherapy, radiotherapy and gene therapy. New strategies, such as immunotherapy, photothermal and photodynamic therapy, magnetic hyperthermia therapy, and multifunctional inorganic nanoplatforms, have also been facilitated by INPs. This review emphasizes the current state of research and clinical applications of INPs, including glioma targeting and BBB penetration enhancement methods, in vivo and in vitro biocompatibility, and diagnostic and treatment strategies. As such, it provides insights for the development of novel glioma treatment strategies.

Guanosine-derived atomically dispersed Cu-N3-C sites for efficient electroreduction of carbon dioxide.

Single-atom copper (Cu) embedded within carbon catalysts have demonstrated significant potential in the electrochemical reduction of carbon dioxide (CO2) into valuable chemicals and fuels. Herein, we develop a straightforward and template-free strategy for synthesizing atomically dispersed CuNC catalysts (CuG) by annealing the self-assembled guanosine. The CuG catalysts display two-dimensional morphology, tunable pore size and large surface areas that can be adjusted by changing carbonization temperature. Spherical aberration-corrected transmission electron microscopy reveals that single-atom Cu are homogeneously dispersed on the surface of carbon nanosheets. The optimum CuG-1000 catalysts achieve a high CO Faradaic efficiency (FEco) up to 99% and a high CO current density of 6.53 mA cm-2 (-0.65 V vs. RHE). Besides, the flow cell test of CuG-1000 shows a high current density up to 25.2 mA cm-2 and the FEco still exceeded 91% after more than 20 h of testing. Specifically, the existence of Cu-N3-C active sites was proved by extended X-ray absorption fine structure (EXAFS). Density functional theory evidences that tricoordinated copper with N can largely regulate the adsorption and desorption of key intermediates by transferring electrons to *COOH through Cu atoms, thereby improving selectivity toward CO. This work demonstrates the active origin of CuNC catalysts in CO2 electroreduction and offers a blueprint to construct atomically dispersed transition site catalysts by supramolecular self-assembly strategy.

Core-shell Mo2C@NC/Mo2C hollow microspheres as highly efficient electrocatalysts for the hydrogen evolution reaction.

Developing low-cost and highly efficient electrocatalysts for the hydrogen evolution reaction (HER) has stimulated extensive interest. Molybdenum carbide materials have been proposed as promising alternatives to noble platinum-based catalysts due to their earth abundance and tunable physicochemical characteristics. Here, we report Mo2C@NC/Mo2C hollow microspheres composed of a ß-Mo2C core and small ß-Mo2C particles embedded within a nitrogen-doped carbon shell and prepared using guanosine and hexaammonium molybdate as precursors via a hydrothermal self-assembly process, which results in outstanding catalytic activity and fast kinetics in hydrogen evolution in both acidic and alkaline solutions. The significant activity improvement of Mo2C@NC/Mo2C can be attributed to the large ratio of exposed active sites and abundant interfacial structures. This work provides a new template-free strategy for the design of a highly active Mo2C@NC/Mo2C hollow microsphere HER catalyst.

Folic Acid-Derived Low-dimensional carbons for efficient oxidative dehydrogenation of ethylbenzene.

The oxidative dehydrogenation (ODH) of alkane is one of the most attractive routes in alkane production because of its favourable thermodynamic characteristic. Nitrogen-doped nanocarbons have demonstrated great potential in this reaction due to its cost-effective, high catalytic activity and stability. However, the influence of nitrogen on the catalytic properties of carbon materials is poorly understood due to the complexities of surface oxygen and nitrogen functional groups. Here we derive the performance descriptor that account for the nitrogen-dependent carbocatalysis in ODH reaction. To achieve this, we designed a set of nitrogen-doped nanocarbon materials with tunable nitrogen species by hydrothermal carbonization (HTC) treatment of the biomass folic acid (FA), which are applied in ODH of ethylbenzene. Among them, FA-180-1000 catalyst can achieve high ethylbenzene conversion (up to ∼62 %) and styrene selectivity (∼87 %), outperforming other HTC carbon-based catalysts. Structural characterizations and kinetic analyses revealed that nitrogen doping strongly interferes the charge polarization of CO site via electron transfer between CO, and nitrogen (mainly pyridine nitrogen and graphitic nitrogen) thus enhancing the reactivity of CO. Furthermore, the induction period during reaction process can be shortened by applying of sulfuric acid-assisted HTC method for constructing nitrogen-doped carbon catalyst with low crystallinity. The present work provides new insights into the contribution of nitrogen doping to the ODH reaction of carbon nanocatalysts, as well as guidance for the rational design of carbon catalysts for the conversion of hydrocarbons to high-value chemicals.

Self-assembly of guanosine into carbon-based multilayer materials.

We report the utilization of guanosine as a supramolecular precursor that unprecedentedly renders the formation of carbon-based multilayer materials with naturally high-level nitrogen doping. As a proof-of-concept, the porous carbon multilayers after anchoring 0.5 wt% Rh electrocatalysts displayed an excellent hydrogen evolution reaction activity.

Effects of microwave radiation on learning and memory and neurotransmitters in the hippocampus / 中国辐射卫生

@#The central nervous system is one of the most sensitive targets of microwave radiation. Microwave radiation can affect spatial learning and memory and neural information transmission. The effects of microwave radiation on neurotransmitters in the hippocampus and the underlying mechanisms are still unclear. This paper reviews the effects of microwave radiation on learning/memory and neurotransmitters as well as the mechanisms of action on neurotransmitters. This paper aims to provide a scientific basis for future research in this area.

Nomogram for prediction of diabetic retinopathy in patients with type 2 diabetes mellitus: A retrospective study.

OBJECTIVE: To develop a nomogram for the risk of diabetic retinopathy (DR) among type 2 diabetes mellitus (T2DM). METHODS: Questionnaires, physical examinations and biochemical tests were performed on 5900 T2DM patients in the Second Hospital of Shijiazhuang. The least absolute shrinkage and selection operator regression was used to optimize feature selection, and the importance of selected features was analyzed by random forest. Logistic regression was performed with selected features, and the nomogram was established based on the results. The Harrell's C-statistic, bootstrap-corrected C-statistic, area under curve (AUC), calibration curve, decision curve analysis (DCA) and clinical impact curve (CIC) were used to validate the discrimination, calibration and clinical usefulness of the nomogram, and further assessment was running by external validation. RESULTS: Predictors included duration of diabetes, diabetic neuropathy, diabetic kidney disease, diabetic foot, hyperlipidemia, hypoglycemic drugs, glycated albumin, Lactate dehydrogenase. The model displayed medium predictive power with a Harrell's C-statistic of 0.820, bootstrap-corrected C-statistic of 0.813 and AUC of 0.820 in the training set, and which was respectively 0.842, 0.835 and 0.842 in the validation set. The calibration curve displayed good agreement (P > 0.05). The DCA and CIC showed that the nomogram could be applied clinically if the risk threshold is between 2 % and 75 % and 2 %-88 % in validation set. CONCLUSIONS: This nomogram incorporating 8 features is useful to predict the risk of DR in T2DM patients.

Impact of the COVID-19 pandemic on household financial asset allocation: A China population study.

During the COVID-19 pandemic, the Chinese government implemented a "dynamic zero" epidemic prevention policy, which led to an increase in the likelihood of business shutdowns, increased uncertainty about people's income, and changes in people's psychological expectations, which in turn influenced their behavioral choices. This study aims to understand the impact of COVID-19 and other major public health emergencies on household financial asset allocation. To do so, we conducted an online survey of 712 people in China to measure household financial asset allocation behavior during three different time periods: pre-pandemic, mid-pandemic, and post-pandemic. At the same time, we analyzed the impact of sociodemographic characteristics on risk attitudes and the differences in household asset allocation decisions at different pre-pandemic time points among people with different risk attitudes. The results show that household financial asset allocation changed significantly before, during, and after the pandemic, and residents' precautionary savings increased. In addition, gender, education level, occupation, and annual income have significant effects on risk preferences. The pandemic leads to increased uncertainty in economic and social development, people's psychological expectations of economic development play an important role in household financial asset allocation.

Interaction effect of blood glucose and pressure on the risk of chronic kidney disease: a population-based prospective cohort study.

OBJECTIVE: To evaluate the interaction effect of blood glucose and blood pressure on the risk of chronic kidney disease (CKD). METHODS: 31,165 subjects were selected without CKD at baseline and had completed the first follow-up from "Jinchang cohort". Cox regression model and restricted cubic splines functions were used to evaluate the effects of blood glucose or pressure on the incidence of CKD and dose-response relationship after adjusting for confounding covariates. Synergic effect was assessed by the multiplicative or additive interaction scale. RESULTS: Among 31,165 subjects, 1307 new-onset CKD were observed during 68905.78 person-years follow-up, and the incidence density was 18.97 per 1000 person-years. The risk of CKD gradually increased with the increase of blood pressure in diabetes, pre-diabetes and normal groups (Ptrend < 0.05). And, the risk was greatest when SBP/DBP reached ≥150/≥110 mmHg in three groups, and HRs (95% CI) were 1.610 (1.070-2.422), 2.142 (1.396-3.288) and 2.455 (1.941-3.106), respectively. Additionally, among hypertension, pre-hypertension and normal groups, the risk of CKD increased by 16.0%, 14.3% and 25.2% for each 1 mmol/L of FPG. When FPG level was more than 9.0 mmol/L, the risk was greatest and adjusted HRs (95% CI) were 2.856 (2.176-3.748), 2.979 (1.828-4.854) and 7.520 (4.517-12.519). Furthermore, the risk was highest when hypertension was accompanied by diabetes (HR = 4.915, 95% CI: 3.923-6.157). This analysis supported a less than multiplicative effect (HR = 0.634, 95% CI: 0.417-0.964) for the interaction term of diabetes and hypertension, while there was no additive interaction towards CKD in all interaction term. CONCLUSIONS: Blood glucose and pressure were independent risk factors in incidence of CKD, but there was only a negative multiplicative interaction between hypertension and diabetes, but no additive interaction effect between them.

Comparing the Diagnostic Criteria of MAFLD and NAFLD in the Chinese Population: A Population-based Prospective Cohort Study.

BACKGROUND AND AIMS: Metabolic dysfunction-associated fatty liver disease (MAFLD) is a new concept, proposed in 2020; however, its applicability in Asia populations has yet to be evaluated. Therefore, we aimed to compare the difference in epidemiological and clinical characteristics between MAFLD and non-alcoholic fatty liver disease (NAFLD) among Asian populations. METHODS: Based on the Jinchang cohort, 30,633 participants were collected. The prevalence and incidence of MAFLD and NAFLD were used to analyze the epidemic characteristics and its overlapping effects. In addition, the corresponding clinical characteristics of the two diagnostic criteria populations were compared. RESULTS: The prevalence rates of MAFLD and NAFLD were 21.03% and 18.83%, respectively. After an average 2.28-year follow-up, the incidence densities of MAFLD and NAFLD were 41.58 per 1,000 person-years and 37.69 per 1,000 person-years, respectively. With the increase of baseline age, body mass index (BMI), and waist circumference (WC) levels, the prevalence and incidence of MAFLD and NAFLD were on the rise (all p trend<0.05). Among the total patients diagnosed at baseline or follow-up, most patients had both MAFLD and NAFLD, accounting for 78.84% and 82.88%, respectively. Compared with NAFLD, MAFLD patients had greater proportions of males and metabolic diseases (diabetes, dyslipidemia), and had higher BMI, WC, liver enzymes, blood glucose, and lipid levels in the baseline diagnosis patients (p<0.05). Additionally, lean MAFLD patients had higher metabolic disorders than lean NAFLD patients (p<0.05). CONCLUSIONS: Compared with NAFLD, the newly proposed definition of MAFLD is more practical and accurate, and it can help identify more fatty liver patients with high-risk diseases.

Effects of thoracic X-ray irradiation on the spermatogenesis of adult male mice / 中华放射医学与防护杂志

Objective:To investigate the effect of thoracic X-ray irradiation on the spermatogenesis of adult male mice.Methods:A total of 24 healthy adult male C57BL/6 mice (6-8 weeks old) were randomly divided into radiation group (Radiation) and sham-radiation group (Sham), 12 mice in each group. The area of thoracic irradiation was 1.5 cm× 2 cm, and the dose rate was 3.04 Gy/min, 8 Gy/d for 3 consecutive days, 24 Gy in total. At 7 d and 21 d after thoracic irradiation, the bilateral testes and epididymal tails were stripped and the testicular index was calculated. The morphology of testis was examined by haematoxylin-eosin (HE) staining, then the diameter of seminiferous tubules and the thickness of seminiferous epithelium were measured. The sperms were collected from the bilateral epididymal tails for sperm counting. The level of apoptosis in testis and levels of apoptosis-related proteins were detected by TUNEL and Western blot, respectively.Results:Compared with Sham group, the morphology of testis and epididymis was seriously damaged, the diameter of seminiferous tubules significantly decreased at 21 d after irradiation ( t = 8.93, P < 0.05), and the seminiferous epithelium significantly decreased at 7 d and 21 d after irradiation ( t = 4.24, 12.77, P < 0.05). In addition, the number of sperms significantly decreased ( t = 4.30, 2.98, P < 0.05). The number of TUNEL positive cells in the seminiferous epithelium significantly increased at 7 d and 21 d after irradiation ( t = -2.73, -3.74, P < 0.05). Meanwhile, the level of cleaved Caspase-3 protein significantly increased at 7 d and 21 d after irradiation ( t = -2.96, -2.46, P < 0.05). The concentrations of SCF and GDNF did not change at 7 d after irradiation, but were significantly increased at 21 d after irradiation ( t = -10.46, -5.42, P < 0.05). Conclusions:The thoracic X-ray irradiation could lead to spermatogenesis disorder in male adult mice, and the induction of spermatogenic cell apoptosis and the secretory dysfunction of sertoli cells may be involved.

Inhibition of ceramide de novo synthesis ameliorates meibomian gland dysfunction induced by SCD1 deficiency.

High expression of stearoyl-CoA desaturase-1 (SCD1) in meibomian glands produces monounsaturated fatty acids that allow the biosynthesis of glycerolipids and other wax-esters but only the low production of sphingolipids. Here, we found that SCD1 deficiency in mice induces the spill of free fatty acids into a parallel pathway for ceramide biosynthesis, resulting in severe meibomian gland dysfunction associated with meibum accumulation in duct lumen and orifices and subsequent atrophy and loss of acinar cells. Genetic and pharmacological inhibition of SCD1 in mice resulted in meibomian gland pathological phenotypes, including local lipid microenvironment alterations, reduced normal cellular differentiation, increased keratinization, inflammatory cell infiltration, cell apoptosis, and mitochondrial dysfunction. However, inhibition of serine palmitoyltransferase, the initial enzyme in ceramide biosynthesis, improved meibomian gland metabolism and morphology in SCD1-deficient mice, resulting in normal cell differentiation and reduced inflammation infiltration, cell apoptosis, and keratinization. These results indicate that elevated levels of endogenous ceramides are a sign of MGD and suggest that inhibition of ceramide de novo biosynthesis could be a new clinical approach to treating MGD.

Single-atom cobalt-fused biomolecule-derived nitrogen-doped carbon nanosheets for selective oxidation reactions.

Non-noble metal single-atom catalysts hold great promise in selective oxidation reactions, although the progress is still unsatisfactory because of the synthesis challenge and the lack of mechanistic interpretations. Herein, we develop a biomolecule-based strategy to synthesize isolated Co single atom site catalysts by one-step pyrolysis of guanosine and Co precursors. Due to the abundant hydrogen bonding and π-π interaction of guanosine, the as-synthesized Co-N-C catalysts present a hierarchical porous two-dimensional (2D) nanostructure with an ultrahigh specific surface area, large pore volume, and high density of cobalt single atoms. Aberration-corrected electron microscopy and X-ray photoelectron spectroscopy reveal that Co species are present as isolated single sites and stabilized by nitrogen-doped carbon nanosheets. These characteristics make Co-GS-900 suitable as an efficient catalyst for selective oxidation of aromatic alkanes. For oxidation of ethylbenzene, Co-GS-900 exhibits a superior performance f with 91% conversion and 98% selectivity of acetophenone.

Application of Whole Process Intelligent Dispensing Mode in the Inpatient Pharmacy / 中国药房

OBJECTIVE：To evaluate the application effects of wh ole process intelligent dispensing mode in the inpatient pharmacy of our hospital ，and to provide reference for the intelligent construction of pharmacy department. METHODS ：Combined with the working situation of inpatient pharmacy in our hospital ，the application of automated drug unit-dose dispensing machine （ADDM），intelligent tablet dispensing table （ITDT），medication detection machine （MDM）were introduced. The improvement of work efficiency and the error rate were compared with before and after the implementation of intelligent dispensing mode. The satisfaction survey of intelligent mode was carried out. RESULTS ：Under the dispensing linkage mode of ADDM+ITDT+MDM ， the time of oral drug dispensing was shortened by about 1.5 h per person ；pharmacists reduced by 2 persons；with the help of ADDM image database and image printing technology of drug bag ，the visual inspection method and the efficiency of drug check could be improved ；the introduction of ITDT standardized the management of dismounted drug ，and the work efficiency of dispensing dismounted drug by ITDT （daily dispensing back-up time as index ）were improved 23.29％-38.93％，compared with manual dispensing. The introduction of MDM improved the efficiency of intelligent check ，all drug information could be traced ， and the work efficiency [daily （total）check time as index] were 65.64％-74.78％ higher than that of manual check ；the error rate of three machine dispensing linkage mode was 70.32％ lower than that of ADDM+manual check and dispensing mode ，52.44％ lower than that of ADDM+MDM linkage mode ，and the overall satisfaction of nu rsing staff was 97.97％. CONCLUSIONS ： ADDM+ITDT+MDM linkage mode shortens dispensing time ， ＊副主任药师 。研究方向 ：医院药学 。电话：0571-56007192。 E-mail：61012796@qq.com improves work efficiency and decreases error rate. # 通信作者 ：副主任药师 。研究方向 ：医院药学 。电话：0571-

[Expression and significance of Ki-67, PI3K and Beclin1 in oral squamous cell carcinoma].

PURPOSE: To detect the expression and significance of Ki-67, PI3K and Beclin1 in oral squamous cell carcinoma (OSCC). METHODS: Thirty patients with OSCC admitted to Nanjing Stomatology Hospital from January 2017 to December 2018 were selected. All specimens were harvested and treated with immunohistochemical staining. The expressions of Ki-67, PI3K and Beclin 1 were detected. Pearson's correlation was used to analyze the correlation among TMSG-1, Ki-67 and Pgp1.The data were analyzed with SPSS 20.0 software package. RESULTS: The positive rates of Ki-67 and PI3K in OSCC tissues were significantly higher than those in adjacent tissues, and the positive rates of Beclin1 were significantly lower than those in adjacent tissues (P<0.05). The positive expression rates of Ki-67, PI3K and Beclin1 in highly differentiated OSCC were significantly higher than those in moderately differentiated and poorly differentiated OSCC (P<0.05). The positive expression rates of Ki-67 and PI3K in OSCC with lymph node metastasis were significantly higher than those in OSCC without lymph node metastasis. The positive expression rates of Beclin1 in OSCC with lymph node metastasis were significantly lower than those in OSCC without lymph node metastasis (P<0.05). The positive expression rates of Ki-67, PI3K and Beclin1 in stageⅠ+Ⅱ and Ⅲ+Ⅳ OSCC were not significantly different (P>0.05). Ki-67 was positively correlated with PI3K (r=0.391, P=0.032), Ki-67 was negatively correlated with Beclin1 (r=-0.525, P=0.02), and Beclin1 was negatively correlated with PI3K(r=-0.367, P=0.045). CONCLUSIONS: Ki-67, PI3K and Beclin1 are correlated with lymph node metastasis and pathological staging. They may be involved in the occurrence and development of OSCC.

Alcohol Induces More Severe Fatty Liver Disease by Influencing Cholesterol Metabolism.

Objectives. Fatty liver disease (FLD) is a major cause of morbidity and mortality worldwide. Dietary cholesterol and alcohol consumption are important risk factors for the progression of FLD, but whether and how alcohol induces more severe FLD with cholesterol ingestion remain unclear. Herein, we mainly used the Lieber-DeCarli diet to establish the FLD mouse model to investigate the synergistic effects of alcohol and cholesterol metabolism on liver damage. The indices of aspartate transaminase (AST), alanine transaminase (ALT), low-density lipoprotein cholesterol (LDL-c), and total cholesterol (TC) levels, inflammation foci, and pathogenesis by hematoxylin and eosin (H&E) and Oil Red O staining revealed that alcohol induces more severe liver damage by influencing cholesterol metabolism, which might be primarily related to the influence of cholesterol absorption, synthesis, and excretion on the liver or small intestine. Moreover, inhibition of absorption of intestinal cholesterol, but not of fat, sucrose, and alcohol, absorption into the body's metabolism by Ezetimibe, significantly improved FLD in rats fed with the high fat-cholesterol-sucrose and alcohol diet. These results showed that alcohol plays an important role in cholesterol metabolism in FLD.