Influence of microplastics on the availability of antibiotics in soils.

Microplastics (MPs) and antibiotics, as two major types of emerging pollutants, inevitably coexist in the soil environment due to agricultural film residue, sewage irrigation and sludge application. However, the impact of MPs on antibiotic availability in soils with varying characteristics has not been extensively studied. Therefore, in this study, an interference experiment was conducted using three types of MPs (polyethylene (PE), polyvinyl chloride (PVC) and polypropylene (PP)) in red soil, paddy soil and cinnamon soil. The available antibiotics in soils were evaluated using diffusive gradients in thin-films (DGT). Results showed that MPs had a significant impact on the amount of antibiotics adsorbed on soil solid (Cs) by providing additional binding sites or altering soil characteristics (e.g., pH and dissolved organic carbon). The most significant effects on Cs were observed in cinnamon soil, and the Cs values were dependent on concentration of MPs. The available antibiotics, as measured by DGT significantly decreased after the addition of MPs. This decrease was influenced by the soil characteristics. However, the concentration of antibiotics in soil solutions (Cd) was only slightly impacted by MPs. Therefore, the influence of MPs on the migration of antibiotics was reflected by their impact on the soil/water partition coefficient (Kd), while the resupply ability (R) from the soil solid phase was less influential. Moreover, the dosage of MPs had a significant effect on the availability of antibiotics in CS by promoting the adsorption of antibiotics on the solid phase, while in RS and PS, the soil properties played a dominate role in the changes in antibiotic availability after MP addition. These results indicate that the impact of MPs on available antibiotics mainly depends on soil properties. In addition, DGT measurement is more sensitive than soil solution to investigate the effects of coexisting pollutants on the behavior of antibiotics in soil.

Construction of a novel fluorescent DNA aptasensor for the fast-response and sensitive detection of copper ions in industrial sewage.

Heavy metal pollution poses a great threat to the ecological environment and human health. In particular, copper ions (Cu2+) play a vital role in regulating fundamental life behavior, and the homeostasis of Cu2+ is closely related to many physiological processes. The excessive accumulation of Cu2+ in the human body through food and drinking water will cause severe diseases. However, current conventional Cu2+ detection methods for evaluating the content of Cu2+ are unable to meet the complete requirements of practical Cu2+ analysis in the practical aquatic environment. In this work, we successfully constructed a novel fluorescent DNA aptasensor, which originated from the binding reaction between the improved DNA fluorescent light-up aptamer termed S2T3AT-GC and a small fluorescent molecule termed DFHBI-1T (S2T3AT-GC/DFHBI-1T) to realize fast and anti-interference response for Cu2+via the competitive interaction between Cu2+ and S2T3AT-GC (Cu2+/S2T3AT-GC) destroying the contained G-quadruplex structure of S2T3AT-GC. Moreover, it enables the sensitive detection of Cu2+ with a detection limit of 0.3 µM and a wide detection linear range from 0.3 to 300 µM. Moreover, with the verification of high stability in real industrial sewage samples, this aptasensor exhibits excellent detection performance for Cu2+ analysis in real water samples. Therefore, the proposed aptasensor exhibits great potential in exploring Cu2+-related environmental and ecological research.

Progression of the tumor in a patient with a gastrointestinal stromal tumor with the PDGFRA exon 12 mutation who received multiple surgeries and multiple lines of tyrosine kinase inhibitor therapies: a case report.

Background: Targeted therapy with tyrosine kinase inhibitors (TKIs) benefits most patients with stromal tumors; however, the effects of TKIs in patients with rare cases of gastrointestinal stromal tumors (GISTs) with platelet-derived growth factor receptor alpha (PDGFRA) exon 12 mutations are unclear. Our report of a case treated with multiline TKIs (included ripretinib) may provide some experience into the future management of rare GIST with PDGFRA 12 exon mutation. Case Description: We report the case of a patient (42-year-old female) with a PDGFRA exon 12-mutated GIST who underwent multiple surgeries and multiple lines of TKI therapy. This patient had intra-abdominal recurrence after imatinib, which was used as the 1st-line targeted drug treatment for 7 months after radical surgery, and had widespread metastases in the abdominal cavity after sunitinib, which was used as the 2nd-line targeted drug treatment for 6 months after the second radical surgery. For this advanced GIST patient with extensive intraperitoneal metastasis and rare PDGFRA 12 exon mutation, we then selected ripretinib as the 3rd-line targeted drug therapy to treat the patient. Up to the last follow-up in September 2021, the patient continued to take drugs without obvious complaints of discomfort or adverse events. Conclusions: This case showed that patients with PDGFRA exon 12-mutated GISTs are less likely to benefit from current conventional TKIs, and ripretinib treatment should be considered preferred to regorafenib or even sunitinib according to each patient's situation. However, the limitation of our case is that the patient's second recurrent lesion was not genetically tested to determine the presence of secondary mutation. Further, if a patient's tumor has a high risk of adverse biological behaviors, such as high mitotic figures, vascular tumor thrombus, succinate dehydrogenase B (SDHB) was negative, and regional lymph node metastasis, consideration should be given to shortening the postoperative follow-up interval to 2 months or even 1 month.

LINC01088 regulates the miR-95/LATS2 pathway through the ceRNA mechanism to inhibit the growth, invasion and migration of gastric cancer cells.

Background: In gastric cancer, a malignant condition with a dismal prognosis, long non-coding RNAs (LncRNAs) play a significant regulatory role. They often compete with microRNAs through the ceRNA mechanism to affect the expression of target mRNA. However, the specific clinical value and mechanism of action of LncRNA in gastric cancer are still unclear. Methods: This study detected the expression and clinical value of LINC01088 in gastric cancer tissues. Furthermore, the biological functions of LINC01088 and the regulation mechanism of the miR-95/LATS2 pathway were explored.Results: LINC01088 and LATS2 mRNA expression decreased, and miR-95 increased in gastric cancer tissues. LINC01088 has an excellent positive correlation with LATS2 mRNA, which may be a ceRNA pair; LINC01088 has binding sites with miR-95. Gene interference tests on gastric cancer cell lines revealed that LINC01088 could prevent gastric cancer cells from proliferating, invading, and migrating. The function of LINC01088 is achieved by regulating the miR-95/LATS2 pathway through the ceRNA mechanism.Conclusion: The results of this study show that LINC01088 expression is significantly reduced in gastric cancer tissues and cell lines. LINC01088 inhibits gastric cancer cells' proliferation, invasion, and migration by regulating the miR-95/LATS2 pathway via the ceRNA mechanism.

UAV remote sensing applications in marine monitoring: Knowledge visualization and review.

With the booming development of information technology and the growing demand for remote sensing data, unmanned aerial vehicle (UAV) remote sensing technology has emerged. In recent years, UAV remote sensing technology has developed rapidly and has been widely used in the fields of military defense, agricultural monitoring, surveying and mapping management, and disaster and emergency response and management. Currently, increasingly serious marine biological and environmental problems are raising the need for effective and timely monitoring. Compared with traditional marine monitoring technologies, UAV remote sensing is becoming an important means for marine monitoring thanks to its flexibility, efficiency and low cost, while still producing systematic data with high spatial and temporal resolutions. This study visualizes the knowledge domain of the application and research advances of UAV remote sensing in marine monitoring by analyzing 1130 articles (from 1993 to early 2022) using a bibliometric approach and provides a review of the application of UAVs in marine management mapping, marine disaster and environmental monitoring, and marine wildlife monitoring. It aims to promote the extensive application of UAV remote sensing in the field of marine research.

Can we quantify the aquatic environmental plastic load from aquaculture?

Aquaculture provides livelihoods for hundreds of millions of people, but it also forms a significant source of plastic litter that poses a serious hazard to aquatic ecosystems. How to assess and subsequently manage plastic loads from aquaculture is a pending and pressing issue for aquaculture sustainability, and an important concern for water environment monitoring and management. In this study, we developed the first framework for estimating plastic litter from aquaculture by combining data from satellite remote sensing, drones, questionnaires, and in situ measurements. By acquiring multidimensional (human and nature) and multiscale (centimeter to basin scale) data, this framework helped us understand the aquaculture farming patterns and its spatial and temporal evolution, and thus estimate the plastic load it generates and suggest effective management approaches. Applying this framework, we assessed the marine plastic load from oyster floating raft farming in the Maowei Sea, a typical mariculture bay in China, with an increasing farming area. Approximately 3840 tons of plastic waste is expected to be discharged into the sea in the next four years (the average service life of a floating raft) without improvements in aquaculture waste management. Strengthening governance, timely plastic removal, innovative replacement, and transforming farmers' behavior patterns are recommended as the subsequent measures for plastic management. This framework can be extended to other regions and other aquaculture patterns, and is applicable to local, regional, and global aquaculture plastic litter assessments. It is a source-based method for evaluating plastic pollution that is more conducive to subsequent plastic management than traditional post-contamination environmental monitoring. In the context of the global expansion of mariculture and the global commitment to action to combat plastic pollution, this approach could play a critical role in the investigation and management of plastic waste in aquatic environments.

Simultaneous nitrification and denitrification by Pseudomonas sp. Y-5 in a high nitrogen environment.

Pseudomonas sp. Y-5, a strain with simultaneous nitrification and denitrification (SND) capacity, was isolated from the Wuhan Municipal Sewage Treatment Plant. This strain could rapidly remove high concentrations of inorganic nitrogen. Specifically, Pseudomonas sp. Y-5 removed 103 mg/L of NH4+-N in 24 h without nitrate or nitrite accumulation when NH4+-N was its sole nitrogen source. The NH4+-N removal efficiency (RE) was 97.26%, and the average removal rate (RR) was 4.30 mg/L/h. Strain Y-5 also removed NO3--N and NO2--N even in aerobic conditions, with average RRs of 4.39 and 4.23 mg/L/h, respectively, and REs of up to 99.34% and 95.81% within 24 h. When cultured in SND medium (SNDM-1), strain Y-5 achieved an NH4+-N RE of up to 97.80% and a total nitrogen (TN) RE of 93.01%, whereas NO3--N was fully depleted in 48 h. Interestingly, high nitrite concentrations did not inhibit the nitrification capacity of Y-5 when grown in SNDM-2, the RE of NH4+-N and TN reached 96.29% and 94.26%, respectively, and nitrite was consumed completely. Strain Y-5 also adapted well to high concentrations of ammonia (~401.68 mg NH4+-N/L) or organic nitrogen (~315.12 mg TN/L). Our results suggested that Pseudomonas sp. Y-5 achieved efficient simultaneous nitrification and denitrification, thus demonstrating its potential applicability in the treatment of nitrogen-polluted wastewater.

Analysis of regional agricultural carbon emission efficiency and influencing factors: Case study of Hubei Province in China.

In recent years, China's industrial economy has grown rapidly and steadily. Concurrently, carbon emissions have gradually increased, among which agricultural production is an important source of greenhouse gas emissions. It is necessary to reduce agricultural carbon emissions by improving their efficiency to achieve the global goal of peak carbon dioxide emissions in 2030. From a dynamic and static point of view, this study puts agricultural carbon emissions into the evaluation index system of agricultural carbon emission efficiency and analyzes the agricultural carbon emission efficiency and its influencing factors in Hubei Province. First, the unexpected output Slacks-based measure (SBM) model in data envelopment analysis was used to evaluate the agricultural carbon emission efficiency of Hubei Province in 2018 and compared it with other provinces horizontally. Second, the Malmquist-Luenberger index was used to analyze the comprehensive efficiency of agricultural carbon emissions in Hubei Province from 2004 to 2018. The role of technological progress and technical efficiency change in the development of low-carbon agriculture in Hubei Province was analyzed. The results showed that agricultural production efficiency in Hubei Province improved from 2004 to 2018, and the overall level was slightly higher than the average level in China. However, agriculture has not eliminated the extensive development modes of high input, low efficiency, high emission, and high pollution. The efficiency of technological progress in agricultural resource utilization in Hubei Province was close to the optimal level. The improvement space was small. Hence, the low efficiency of agricultural technology is a key factor restricting the improvement of agricultural production efficiency. The results provide a reference for low-carbon agricultural policy formulation and expand the policy choice path. This has practical significance.

Simulation of the effects of microplastics on the microbial community structure and nitrogen cycle of paddy soil.

Microplastics (MPs) are ubiquitous in farmland soils. However, few studies have evaluated their effects on the microbial community structure and nitrogen cycle of farmland soils. Here, 0.3% and 1% (mass percentage) of polyethylene terephthalate (PET), polyvinyl chloride (PVC), and polylactic acid (PLA) MPs were added to paddy soil to evaluate their impact on the paddy soil microenvironment. The alpha index of the PLA MP treatment was significantly different from that of the control group (p-value < 0.05). In contrast, the indices of the PET and PVC MP treatments were not different from the control (p-value > 0.05). Among the MP treatments, the alpha index of the PLA MP group was significantly different from the PET and PVC MP groups (p-value < 0.05). PCoA analysis also indicated that there were differences between PLA and other MP groups, and different MP concentrations and exposure times had a great impact on microbial composition. The three MPs affected NH4+ metabolism by changing the abundance of a NH2OH-forming gene (amoA) and an organic nitrogen-forming gene (gdh), as well as the abundances of Thiobacillus, Bradyrhizobium, Anaeromyxobacter, Geobacter, and Desulfobacca. Further, the MPs affected NO3- metabolism by regulating the abundance of the nirS and nirK genes and the abundance of Nitrospirae. In contrast, NO2- metabolism was not significantly affected by the MPs due to the low concentration of NO2-, which was attributed to the high abundance of nirS and nirK in the sample. Taken together, our findings indicated that MP addition may have an inhibitory effect on the nitrogen cycle in paddy soils and that the effect of degradable MPs may be greater than that of their non-degradable counterparts. Given the increasing severity of worldwide MP contamination, additional studies are required to assess their impact on global ecosystems and biogeochemical cycles.

Guest-dependent directional complexation based on triptycene derived oxacalixarene: formation of oriented rotaxanes.

The manipulation of supramolecular devices to carry out sophisticated and programmed tasks is bound up with the spatial allocation of their components, especially the threading direction of the guest, which controls the host-guest orientation in the device. However, insights are needed to probe more possibilities for steering the threading direction. We have developed a new system consisting of a three-dimensional nonsymmetric oxacalixarene (H) with a fixed comformation and (bi)pyridinium salts (G1-G3), in which we found that based on the intrinsic discrepancies between the two semi-cavities of H, the electron densities of the axles greatly affect the threading direction. This was unequivocally demonstrated by NMR spectra and single crystal structures. With elaborate design, unidirectional threading was achieved, resulting in an oriented rotaxane. Therefore, we describe a new approach in which the threading direction and final orientation may be finely controlled by adjustment of the structure of the guest.