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Objective To investigate the value of thrombomodulin(TM),thrombin-antithrombin complex(TAT),α2 plasmin inhibitor-plasmin complex(PIC)and tissue plasminogen activator-inhibitor complex(t-PAIC)in the diagnosis and prognosis of neonatal disseminated intravascular coagulation(DIC).Methods Eighty-seven DIC neonates(the observation group)were included and divided into the survival group(66 cases)and the death group(21 cases)based on their outcomes at discharge.And 50 healthy newborns born in the same period were selected as the control group.The clinical data of neonates were collected,and risk factors of neonatal DIC were analyzed by Logistic regression.The differences of TM,TAT,PIC and t-PAIC levels in different groups were analyzed.The receiver operating characteristic(ROC)curve was used to analyze values of TM,TAT,PIC and t-PAIC in the diagnosis and prognosis of neonatal DIC.Results The incidence of low Apgar score,birth asphyxia,IVH,sepsis and maternal pregnancy induced hypertension syndrome(PIH)were higher in the observation group than those in the control group(P＜0.05).Multivariate Logistic regression analysis showed that low Apgar score,birth asphyxia,sepsis and PIH were independent risk factors for neonatal DIC.TM,TAT,PIC and t-PAIC levels were higher in the observation group than those in the control group(P＜0.05).ROC curve showed that the combined diagnosis value of TM,TAT,PIC and t-PAIC was better than that of single diagnosis of neonatal DIC.TM and TAT levels were higher in the death group than those in the survival group(P＜0.05),and there were no significant differences in PIC and t-PAIC levels between the two groups.Multivariate Logistic regression analysis showed that elevated TAT level was an independent risk factor for neonatal DIC prognosis.ROC curve showed that when TAT was 21.72 μg/L,the area under the curve for predicting neonatal DIC prognosis was 0.772(95%CI:0.666-0.878),and the sensitivity and specificity were 76.2%and 71.2%,respectively.Conclusion The combined application of TM,TAT,PIC and t-PAIC has important clinical value in diagnosis and prognosis evaluation of neonatal DIC.
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Objective:To study the relationship between atrophy of the thymus and disease severity in EAE.Methods:MOG35-55 peptide induced EAE in C57BL/6 mice and analyzed the relationship between the severity of EAE and thymic atrophy,Flow cytometry analysis was used to evaluate thymic CD4+CD8+DP cells,CD4+CD8-,CD4-CD8+SP cells in relation to the severity of the disease.Results:The number of thymocytes in mice with decreased tail tone was (20.25 ±3.49) ×106 ,hindlimb weakness(4.93 ± 0.85)×106,complete hindlimb paralysis(1.8 ±0.19) ×106,and forelimb and hindlimb paralysis(0.52 ±0.07) ×106,there were statistically significant differences between groups ( P<0.05 ).As the disease progresses, CD4+CD8+DP cells ratio decreased, CD4+CD8-,CD4-CD8+SP cell ratio increased,different disease groups was statistically significant difference (P<0.05).Conclusion: The atrophy of thymus was closely related to the severity of EAE.Migration of activated T cells in EAE may cause atrophy of thymus.
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Objective To evaluate the first-line and second-line anti-tuberculosis drugs resistance of clinical Mycobacterium tuberculosis ( M. TB) isolates and the prevalence of Extensively Drug-Resistant Tuberculosis (XDR-TB) in hospital-base TB patients in Shandong province, China. Methods Absolute concentration method based on egg medium were examined for drug-resistance of M. TB strains to 4 first-line anti-tuberculosis drugs (INH, RFP, SM, and EMB) and 5 second-line drugs (FQ,CPM,AMK,and PAS).Results 1021 isolates were obtained from clinical TB patients at Shandong Provincial Chest Hospital during November 2004 to April 2007. Of those, 32 strains ( 3.1% ) were identified to Mycobacterium other than tuberculosis. Among 989 M. TB complexe isolates, the general resistance rate to first-line drugs were 32. 3% (95% CI:28.7% - 35.8% ), 107 isolates ( 10. 8% ) were found to be MDR-TB. According to the revised Global XDR-TB Task Force definition, a total of 20( 18. 7% ) MDR-TB patients met criteria for XDR-TB. All XDR-TB patients were previously treated pulmonary tuberculosis cases. Conclusions The prevalence of resistant to second-line anti-tuberculosis drugs in Mycobacterium tuberculosis was very serious, and high prevalence of XDR-TB in hospital-base patients was found in high-level The study showed that it is important to successively survey the anti-tuberculosis drug resistance in clinical laboratory.