RESUMO
A sensitive DC magnetic field sensor is constructed by measuring the signal-to-noise ratio of an AC-modulated magnetic field at a particular frequency from an optical whispering gallery mode microcapillary resonator. The sensing element consists of an optical whispering gallery mode microcapillary resonator bonded to a magnetostrictive material that enables it to respond to external magnetic fields. A DC magnetic field sensitivity of 0.1703dB/Oe and a linear detection range from 4.8Oe to 65.7Oe are realized under an AC modulation field of 168.1kHz in the unshielded environment at room temperature. To our best knowledge, this sensitivity is about 2.3 times of the maximum sensitivity of other DC magnetic field sensors based on magnetic fluid or magnetostrictive material integrated fiber systems that use the dissipative sensing scheme. Furthermore, the sensor can operate at a stable temperature in the range of [-11â¼45]°C, as long as the modulation frequency of the AC-modulation field is adjusted according to the ambient temperature. This sensor provides us with a novel DC magnetic field sensing scheme, which may play a role in industrial fields related to current and position detection in the future.
RESUMO
A cosmid clone cZFYN1413 with CMCase activity was identified from a soil metagenomic library. The sequence analysis of a subclone of cZFYN1413 revealed an endo-ß-1,4-glucanase gene ZFYN1413 belonging to glycoside hydrolase family 6 and a transmembrane region in the N-terminal of ZFYN1413. Expression of ZFYN1413 in Escherichia coli BL21 (DE3) resulted in ZFYN1413-87, which was a truncated protein cleaved in transmembrane region of ZFYN1413. ZFYN1413-87 was expressed and its enzyme properties were studied. ZFYN1413-87 possessed strong endo-ß-1,4-glucanase activity, and 52% of the activity could be retained after the protein was treated in buffer of pH 3.0 for 2 h. The study provided a special example of endo-ß-1,4-glucanase in GH6 family.
