RESUMO
ABSTRACT: The aim of this study was to investigate the correlation between single-nucleotide polymorphisms (SNPs) in the 3 primer of untranslated region (3'UTR) of the Pentraxin 3 (PTX3) gene and the risk of essential hypertension (EHT).PTX3 genotypes, rs2614, rs111451363, and rs73158510 locus, were found in 260 patients with EHT and 260 healthy controls. Quantitative real-time polymerase chain reaction was used to detect plasma hsa-miR-4766-5p levels. Enzyme-linked immunosorbent assay was used to detect plasma PTX3 levels. The dual-luciferase reporter assay was used to identify the binding site of hsa-miR-4766-5p to the PTX3.PTX3 rs2614 locus T allele was a high risk factor for EHT (odds ratio [OR]â=â2.76, 95% confidence interval [CI]: 1.86-4.09, Pâ<â.01). Sex and diabetes history affected the correlation between PTX3 gene rs2614 locus SNP and EHT risk. The CCG haplotype was a protective factor for EHT (ORâ=â0.40, 95% CI: 0.28-0.57, Pâ<â.01), whereas the TCG haplotype was a risk factor for EHT (ORâ=â2.35, 95% CI: 1.51-3.66, Pâ<â.01). The plasma PTX3 level of patients with EHT was significantly higher than that of the control group, and the difference was statistically significant (Pâ<â.01). The area under the curve for EHT diagnosis in plasma PTX3 levels was 0.62 (95% CI: 0.57-0.66, Pâ<â.01). The plasma hsa-miR-4766-5p level in patients with EHT was significantly lower than that in the control group (Pâ<â.01). The area under the curve for the diagnosis of EHT according to the plasma hsa-miR-4766-5p level was 0.88 (95% CI: 0.85-0.91, Pâ<â.01). Plasma PTX3 levels were significantly negatively correlated with hsa-miR-4766-5p levels in patients with EHT and the control group (râ=â-0.87, -0.85, Pâ<â.01, Pâ<â.01). The PTX3 gene rs2614 locus C allele was the target gene of hsa-miR-4766-5p.The PTX3 rs2614 locus SNP is significantly associated with EHT risk.