[Analysis of HLA-DQB1 polymorphism for patients with allergic rhinitis of Uygur and Han people in Xinjiang].

OBJECTIVE: To investigate the association of HLA-DQB1 alleles and artemisia pollen allergic patients of Han and Uygur people in the Xinjiang area. METHOD: PCR-SSP (sequence-specific primer polymerase chain reaction) were used to test fifty Uygur and fifty Han people with allergic rhinitis with hypersensitivity to artemisia pollen in the Xinjiang area. Fifty normal Uygur and fifty normal Han people in the Xinjiang region were enrolled in the control group. RESULT: The frequency of HLA-DQB1 * 05 in the Uygur group with allergic rhinitis was significantly higher (19.4%) than that in the Uygur control group (7%) (P < 0.05). There were no significant difference between in the allergic rhinitis group and in the control group. CONCLUSION: HLA-DQB1 * 05 might be the susceptibility genes of the Uygur patients with allergic rhinitis in the Xinjiang area.

[Study on the relationship between SNP of CD14 gene and allergic rhinitis in Xinjiang Uygurs and Hans].

OBJECTIVE: This study aimed to investigate the relationship between CD14 gene rs2569192(C/G), rs3138078 (--1359G/T) and allergic rhinitis (AR) in Xinjiang Uygur and Han populations as well as to determine characteristics of polymorphisms. METHOD: A total of 300 AR and 300 healthy controls subjects were included. The frequencies of genotypes and alleles were detected as well as the levels of tIgE in different genotypes were compared. RESULT: (1) The distribution of genotypes or alleles of CD14 gene rs2569192 (C/G), rs3138078 (--1359G/T) had no differences between the Xinjiang Uygurs and Hans (P > 0.05). The highest frequency of alleles was C, G. (2) The frequencies of genotypes and alleles were not different between the AR and control group in Uygur and Han (P > 0.05). The frequencies of genotypes and alleles of rs2569192 were different between the Uygur AR and Han AR group (P < 0.05). (3) The distribution of genotype frequencies and allele of rs 2569192 in the Xinjiang Uygur and Han population were quite different from Chinese Beijing Han populations, Japanese, European and African (P < 0.05). (4) The serum total IgE level in AR group was higher than that in healthy control group (P < 0.05). CONCLUSION: (1) rs2569192 (C/G), rs3138078 (--1359G/T) polymorphisms were not different between the Chinese Xinjiang Uygur and Han population. The major allele were both C and G. rs2569192 of CD14 in Xinjiang populations was different from that in the other populations. (2) No relationship between rs2569192, rs3138078 and AR was found. (3) The serum total IgE level in AR group was higher than that in healthy control group. No relationship between CD14 two SNP and serum total IgE level was found.

[Treatment of allergic rhinitis with asthma by different ways of inhaling corticosteroids].

OBJECTIVE: To observe the therapeutical effect on allergic rhinitis with asthma by different ways of inhaling corticosteroids. METHODS: Ninety patients suffering from allergic rhinitis with asthma were classified into three groups in random and treated with budesonide (BUD) by nasal spray, inhaling and nasal spray-inhaling combined administration for 12 weeks, respectively. The concentration of serum total IgE, eosinophil cationic protein (ECP) and IL-5, pulmonary functions were examined before and after treatment. RESULTS: The improvement of symptom scores of rhinitis and asthma in three groups were significant (P < 0.05). The concentration of serum total IgE, ECP and IL-5 in three groups decreased greatly after treatment (P < 0.05). There were significant improvement in FEV1, FEV1/FVC and FEF25%-75% in inhaling group before and after treatment (P < 0.05): FEV1 from (2.04 +/- 0.45) L to (2.47 +/- 0.54) L, FEV1/FVC from (72.73 +/- 5.59)% to (75.42 +/- 5.94)% and FEF25%-75% from 1.69 +/- 0.52 to 2.06 +/- 0.77. There was also significant improvement in nasal spray-inhaling combined groups before and after treatment (P < 0.05): FEV1 from (2.32 +/- 0.56) L to (2.76 +/- 0.58) L, FEV1/FVC from (73.80 +/- 4.17)% to (76.04 +/- 4.49)% and FEF25%-75% from 2.09 +/- 0.45 to 2.34 +/- 0.64. The significant difference of FEV1 among the three treatment groups was observed (P = 0.041). CONCLUSIONS: The symptoms of rhinitis and asthma in three groups by three ways of inhaling BUD were greatly improved, no significant difference in symptom scores and serum parameters was found.

[A study on the relationship between interleukin-4 promoter polymorphism and asthma in a Xinjiang Uyger population].

OBJECTIVE: To investigate the potential association of interleukin-4 (IL-4) polymorphism-589 (C/T) with specific clinical phenotypes of asthma in Xinjiang Uygur population. METHODS: Ninety-three Uyger asthmatics [asthmatic group (A), mild 30 (A(1)), moderate 32 (A(2)), severe 31 (A(3)); nocturnal asthma 41 (A(I)), non-nocturnal asthma 52 (A(II))] in whom linkage of asthma and atopy to chromosome 5q(31 - 33) was suggested and 62 normal healthy controls (B group) with the same ethnicity nearby Xinjiang Hetian region were investigated. The IL-4-589 (C/T) polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The serum eosinophil cationic protein (ECP) and total IgE and specific aeroallergens were detected using a Pharmacia UniCAP 100 type instrument, and pulmonary ventilatory function was measured by FEV(1)% and PEF. RESULTS: The IL-4 polymorphism-589 (C/T) frequency of the heterozygous CT was 42 (45.1%), homozygous CC 29 (31.2%), and homozygous TT 22 (23.7%) in A group. The frequency of the heterozygous CT was 26 (41.9%), homozygous CC 21 (33.9%), and homozygous TT 15 (24.2%) in B group. There were no significant differences in the distributions of the three genotypes between A group and B group (P > 0.05). The frequency of the homozygous CC was 39.0% in the A(I) group as compared to 25.0% in the A(II) group, the difference being significant (P = 0.026). The frequency of the heterozygous CT was 40% (12/30) in A(1) group, 41% (13/32) in A(2) group and 55% (17/31) in A(3) group. There were significant differences in the distributions of the three genotypes among A(1), A(2) and A(3) group (all P < 0.05). The total serum IgE levels were (75.2 +/- 43.2) kU/L and (52.3 +/- 22.4) kU/L in A group and B group respectively, and there was no significant difference in the levels of total serum IgE among the genotypes in both A and B groups (P > 0.05). The serum ECP levels were (7.9 +/- 5.4) microg/L and (4.8 +/- 1.9) microg/L in A group and B group respectively, the difference being significant between the two groups (P < 0.01). The positive rate of specific IgE antibody was 18.3% (17/93), and 0% (0/62) in A group and B group respectively, and there was significant difference between the two groups (P < 0.01). CONCLUSIONS: It is suggested that the IL-4 promoter-589 (C/T) polymorphism may be associated with nocturnal phenotypes of asthma, but not associated with pulmonary ventilatory function (P > 0.05) and serum total IgE level in this Xinjiang Uyger population.