Advances in single-cell RNA sequencing in the retina / 中华眼底病杂志

Retina is composed of a heterogeneous population of cell types, each with a unique biological function. Even if the same type of cells, due to genetic heterogeneity will lead to cell function differences. In the past, traditional molecular biological methods cannot resolve variations in their functional roles that arise from these differences, and some cells are difficult to define due to the lack of specific molecular markers or the scarcity of numbers, which hindered the understanding and research of these cells. With the development of biotechnology, single-cell RNA sequencing can analyze and resolve differences in single-cell transcriptome expression profiles, characterize intracellular population heterogeneity, identify new and rare cell subtypes, and more definitely define the characteristics of each cell type. It clarifies the origin, function, and variations in cell phenotypes. Other attributes include pinpointing both disease-related characteristics of cell subtypes and specific differential gene expression patterns, to deepen our understanding of the causes and progression of diseases, as well as to aid clinical diagnosis and targeted therapy.

The effect of form deprivation on the morphology of retinal ganglion cells in mice / 中华眼底病杂志

Objective To investigate the effects of form deprivation on the morphology of different types of RGC in mice.Methods Sixty B6.Cg-Tg (Thy1-YFP) HJrs/J transgenic mice were randomly assigned to form-deprived group (n=28) and control group (n=32). The right eyes of mice in the form-deprived group were covered by an occluder for 2 weeks as experimental eyes. The right eyes of mice in the control group were taken as control eyes. Before and 2 weeks after form deprivation, the refraction and ocular biometrics were measured; RGC were stained with Bra3a antibody and counted; the morphology of RGC was reconstructed with Neuroexplore software after immunohistochemical staining. The data was compared among experimental eyes, fellow eyes and control eyes by one-way analysis of variance.Results Two weeks after form deprivation, the axial myopia was observed in the experimental eyes (refraction:F=15.009,P<0.001; vitreous chamber depth:F=3.360,P=0047; ocluar axial length:F=5.011,P=0013). The number of RGC in central retina of the experimental eyes was decreased compared with the fellow eyes and the control eyes (F=4.769,P=0.035). The reconstructed RGC were classified into 4 types according to their dendritic morphology. Form deprivation affected all 4 types of RGC but in a different way. Among them, 3 types of RGC were likely contribute to form vision perception. Form deprivation increased the dendrite branches in these types of ganglion cells. However, form deprivation decreasd dendrite segment numbers in both eyes and the intersection and length insholl analyse type 4 ganglion cells which were morphologically identified as ipRGC.Conclusion Form deprivation distinguishingly affects the morphology of different types of RGC, indicating that form vision and non-form vision play different role in myopia development.

Development and application of scoring system for academic conference papers / 中华医学科研管理杂志

Objective To develop a Scoring System for Academic Conference Papers,in order to achieve real-time paper scoring in large academic conference with the information technology and network.Methods Based on Microsoft.Net technology,an information system Was developed,supporting multimeeting.multi-client and dynamic screen show.Results In this system,the conference inforrnation,meeting name and scoring standards Call be customized;the doctor information,papers information,used time,score detail and dynamic list Can be displayed in the screen.This system has been applied in national ophthalmic conferences several times and gained praise from the organizing committee and judges.Conclusions The scoring system for Academic Conference Papers can significantly improve efficiency,help to promote the standardization of paper scoring,and manifest the open,fair and just principles.It is worth of spreading.

Using basement membrane of human amniotic membrane as a cell carrier for cultivated cat corneal endothelial cell transplantation.

PURPOSE: To study the feasibility of using basement membrane of human amniotic membrane (BMHAM) as a carrier for transplantation of cultivated cat corneal endothelial cells (cCCECs). METHODS: BHMAM was obtained by enzymic digestion. cCCECs were seeded on the BMHAM and cultivated traditionally. The resulting continuous monolayer of cCCECs was transplanted onto the cat corneal graft stripped of the Descemet membrane with endothelium. To determine whether the transplanted cCCECs were vital and functional in vivo, the corneal grafts were examined by slit-lamp microscope every day for 6 weeks, and corneal thickness was measured by ultrasonic pachymetry. Either in vivo or in vitro, the cCCEC sheets on BMHAMs were examined morphologically by light and electron microscope, and the cell density was measured. RESULTS: Seven to 10 days after seeding on the BMHAM, the cCCECs were confluent and formed a continuous monolayer with 3486 +/- 53 cells/mm(2) cell density. Like normal corneal endothelial cells, the cCCECs were almost hexagonal, squamous, and uniform in size. After transplantation, most cells were vital and functional nearly enough to maintain corneal graft thickness and transparency without rejection for at least 6 weeks. Six weeks after operation, the average thickness of the transplanted corneal grafts was only slightly greater than that before operation. Compared with that in vitro, after transplantation there was 5% to 8% reduction per week in cell density, which lasted for almost 3 weeks. After that, the average cCCEC density of corneal grafts was 2837 +/- 57 cells/mm(2) and quite stable maintained. CONCLUSIONS: This study demonstrated that BMHAM would be an ideal alternative for corneal Descemet membrane and a cell carrier for cCCEC transplantation.