RESUMO
A new phenylpropanoid, (+)-methyl 3-acetoxy-3-(7-methoxy-1,3-benzodioxol-5-yl) propanoate (1), was obtained from the 95% EtOH extract of entire plant of Peperomia tetraphylla. The structure was elucidated based on the spectral analysis (IR, 1-D and 2-D NMR and HRESIMS). The absolute configuration of 1 was determined as (3R) by referring to analogous compound's optical rotation. Cytotoxicity assays showed that compound 1 had a moderate inhibitory activity against the A549 cell line, weak inhibitory activity against the Hela and the HepG2 cell lines.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Peperomia/química , Fenilpropionatos/química , Antineoplásicos Fitogênicos/química , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Células Hep G2 , Humanos , Concentração Inibidora 50 , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Fenilpropionatos/isolamento & purificação , Fenilpropionatos/farmacologia , Extratos Vegetais/química , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
OBJECTIVE: To study the triterpenoids constituents in Potentilla discolor. METHODS: The compounds were isolated by silica gel chromatography, macroporous resins and polyamide column chromatography from the 70% ethanol extract, and their structures were identified by spectral analysis. RESULTS: Six compounds were obtained and their structures were identified as 3-O-beta-D-glucopyranosyl-(1 --> 2) -beta-D-xylopyranosyl-19a-hydroxyurs-12-en-28-acid (1), 2alpha, 3/beta, 19alpha-trihydroxyurs-12-en-28-acid (2), 3beta, 19alpha-trihydroxyurs-12-en-24, 28-acid (3), 2alpha, 3beta-dihydroxyolean-12-en-28-acid (4), 2alpha, 3alpha, 19alpha-trihydroxyurs-12-en-28-acid (5), beta-sitosterol (6). CONCLUSION: Compounds 1 and 3 are obtained from this plant for the fisrt time.
