RESUMO
MicroRNAs (miRNAs) are small, non-coding RNAs that post-transcriptionally regulate gene expression. Aberrant miRNA expression or function have close links with various human diseases. Therefore, therapeutic treatments with disease-associated miRNAs as targets are emerging. However, the intracellular miRNA networks are extremely complicated and poorly understood, which thus hinder the development of miRNA-targeted therapeutics. Small molecules that are able to regulate endogenous miRNAs hold great potential in both elucidation of miRNA networks and treatment of miRNA-related diseases. Herein, we summarize current strategies for discovery of small molecule modifiers of miRNAs, and we highlight aspects of miRNA cellular biology elucidated by using these small molecules and miRNA-targeted therapeutics realized by these small molecules. We envision that this area will expand dramatically in the near future and will ultimately contribute to a better understanding of miRNA-involved cellular processes and development of therapeutic agents for miRNA-associated diseases.
Assuntos
Tratamento Farmacológico , MicroRNAs/fisiologia , Biologia Computacional/métodos , Doença/genética , Humanos , MicroRNAs/química , MicroRNAs/efeitos dos fármacos , MicroRNAs/genética , Conformação de Ácido Nucleico/efeitos dos fármacosRESUMO
The zinc finger protein Snail1 is an important factor in the regulation of the epithelialmesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells. The present study demonstrated that the expression of Snail1 in HCC tissues was significantly higher compared with its expression in tissues adjacent to primary sites, as determined via western blotting. Furthermore, the results of a dual luciferase assay revealed that hsamicroRNA(miR)199a5p negatively regulated the protein expression of Snail1 by binding to its 3' untranslated region. However, in a comparative analysis of primary HCC and its metastatic tissues using reverse transcriptionquantitative polymerase chain reaction and western blotting, it was demonstrated that the expression of hsamiR199a5p and Snail1 in HCC metastatic tissues were significantly higher compared with primary lesions and an association between them identified that hsamiR199a5p lost its ability to negatively regulate Snail1. This result is contradictive to the fact that hsamiR199a5p inhibits the expression of the Snail1 protein. The present study hypothesized that the aberrant expression of long noncoding RNA was the cause of hsamiR199a5p inactivation based on loss of function rather than a reduction in content. The data collected in the present study confirmed the hypothesis that AB209371 binds to hsamiR199a5p and weakened the inhibitory effect of hsamiR199a5p on Snail1 expression. In addition, an in vitro EMT model was established in the present study by inducing HCC cells with TGFß1. The results revealed that AB209371 silencing effectively reversed the hsamiR199a5p mediated inhibition of EMT by negatively regulating Snail1 protein expression. Therefore, AB209371 silencing in combination with hsamiR199a5p expression may serve as an effective means to inhibit EMT in HCC cells. The present study also revealed that hsamiR199a5p/Snail1 exhibits a dominant regulatory effect in the EMT of HCC cells via a Snail1 recovery experiment. In conclusion, to the best of our knowledge, the present study confirmed for the first time that the high expression of AB209371 is favorable for the EMT in HCC cells and may be a direct cause of hsamiR199a5p inactivation (an HCC metastasis suppressor). Additionally, AB209371 silencing combined with hsamiR199a5p overexpression may be an effective means to inhibit the metastasis of HCC and the EMT of HCC cells.