The effect of white spot syndrome virus (WSSV) envelope protein VP28 on innate immunity and resistance to white spot syndrome virus in Cherax quadricarinatus.

VP28 is the most abundant membrane protein of WSSV, and the recombinant protein VP28 (VP26 or VP24) was constructed for the immune protection experiment in this study. Crayfish were immunized by intramuscular injection of recombinant protein V28 (VP26 or VP24) at a dose of 2 µg/g. The survival rate of crayfish immunized by VP28 showed a higher value than by VP26 or VP24 after WSSV challenge. Compared with the WSSV-positive control group, the VP28-immunized group could inhibit the replication of WSSV in crayfish, increasing the survival rate of crayfish to 66.67% after WSSV infection. The results of gene expression showed that VP28 treatment could enhance the expression of immune genes, mainly JAK and STAT genes. VP28 treatment also enhanced total hemocyte counts and enzyme activities including PO, SOD, and CAT in crayfish. VP28 treatment reduced the apoptosis of hemocytes in crayfish, as well as after WSSV infection. In conclusion, VP28 treatment can enhance the innate immunity of crayfish and has a significant effect on resistance to WSSV, and can be used as a preventive tool.

Immunotoxicity of polychlorinated biphenyls (PCBs) to the marine crustacean species, Scylla paramamosain.

Polychlorinated biphenyls (PCBs) are persistent organic pollutants in environments, and they can negatively affect aquatic animal health. After 7 days of PCBs exposure, the activities of catalase, phenoloxidase, and superoxide dismutase and the total hemocyte count in the haemolymph were significantly decreased and the reactive oxygen species (ROS) content and phagocytic rate of hemocytes were significantly increased in mud crab Scylla paramamosain. Additionally, serum lysozyme, glutathione, glutathione-S-transferase, and glutathione peroxidase activities were significantly down-regulated in mud crab after PCBs exposure. The survival rate of crab hemocytes significantly declined as the PCBs concentration increased, indicating that PCBs had a cytotoxic effect on hemocytes. Exposure to increasing concentrations of PCBs also increased the degree of DNA damage in crab hemocytes. After PCBs exposure, the expression levels of P53 and caspase-3 in hemocytes were significantly up-regulated, which suggests that apoptosis was occurring. The apoptosis rate of hemocytes was up-regulated as the PCBs concentration increased, indicating that apoptosis was induced by the PCBs-activated caspase-3 pathway. These data suggest that exposure to PCBs hampered the immune response of mud crabs, most likely by (1) inducing ROS, causing DNA damage, and reducing the viability of hemocytes, (2) reducing the activities of antioxidant enzymes, and (3) inducing phagocytosis and apoptosis of hemocytes. And the final result of PCBs-induced immunotoxicity to mud crabs is the reduced bacterial disease resistance and survival rate of crabs under Vibrio alginolyticus challenge.

Effects of dietary quercetin on the innate immune response and resistance to white spot syndrome virus in Procambarusclarkii.

In recent years, the use of natural products with immune-stimulating and antimicrobial properties has attracted increasing attention in aquaculture researches. In our study, the effect of diet supplemented with quercetin, a flavonoid commonly found in some types of plants substance on the innate immune response and disease resistance in crayfish (Procambarus clarkii) against white spot syndrome virus (WSSV) is reported. It was found that dietary 40 mg/kg quercetin significantly reduced the mortality of crayfish and WSSV copy number after WSSV challenge. Dietary quercetin increased catalase (CAT), and lysozyme (LZM) activity in crayfish. Dietary quercetin increased the expression of NF-κB, anti-lipopolysaccharide factor (ALF) and toll-like receptor (TLR) genes in crayfish. The apoptosis rate of hemocyte was increased by quercetin supplement in crayfish. Our results suggest that dietary quercetin may affect the innate immunity of crayfish and protect crayfish from WSSV infection.