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1.
J Vis Exp ; (147)2019 05 20.
Artigo em Inglês | MEDLINE | ID: mdl-31157761

RESUMO

The age of a submarine hydrothermal sulfide is a significant index for estimating the size of hydrothermal ore deposits. Uranium and thorium isotopes in the samples can be separated for 230Th-U dating. This article presents a method to purify and separate U and Th isotopes in submarine hydrothermal sulfide samples. Following this technique, the separated U and Th fractions can meet measuring requirements by multi-collector inductively coupled plasma mass spectrometry (MC-ICPMS). The age of the hydrothermal sulfide sample can be calculated by measuring the present-day activity ratios of 230Th/238U and 234U/238U. A super clean room is necessary for this experiment. Cleaned regents and supplies are used to reduce the contamination during the sample processes. Balance, hotplate, and centrifuge are also used. The sulfide sample is powdered for analysis and less than 0.2 g sample is used. Briefly, the sample is weighed, dissolved, added to 229Th-233U-236U double spike solution, Fe co-precipitated, and separated on an anion-exchange resin extraction column. Approximately 50 ng U is consumed for 230Th-U dating of sulfides sample by MC-ICPMS.


Assuntos
Fontes Hidrotermais/química , Sulfetos/análise , Tório/isolamento & purificação , Urânio/isolamento & purificação , Navios , Análise Espectral , Tório/análise , Urânio/análise
2.
Mar Pollut Bull ; 133: 701-710, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30041367

RESUMO

To understand the effect of metal pollution on the speciation process, we conducted comparative analyses of six populations of the gastropod Melanoides tuberculata, which dominated the Manzala lagoon (Nile Delta, Egypt). Geometric morphometric analysis was implemented to quantify the phenotypic plasticity of the species. The results from both Canonical Variate Analysis and Relative Warp indicated an overall decrease in the morphological breadth of M. tuberculata in the polluted sites. The favored phenotypes in the polluted sites have moderate whorl section, moderate ovate aperture, less-prominent radial ornament, and overall moderate-spired shells. Lack of morphological variations and dominance of intermediate phenotypes in the polluted sites indicate that stabilizing selection is driving the morphological pattern of this species. Moreover, analysis by using the partial least square model confirmed that metal pollution is the major predictor of the observed shape variations, whereas other biotic/abiotic traits are a minor predictor.


Assuntos
Ecotoxicologia/métodos , Seleção Genética , Caramujos/fisiologia , Animais , Egito , Sedimentos Geológicos/análise , Lagos , Metais/análise , Metais/toxicidade , Fenótipo , Caramujos/anatomia & histologia , Caramujos/efeitos dos fármacos , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
3.
Sci Rep ; 5: 8001, 2015 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-25614046

RESUMO

The Holocene Warm Period (HWP) provides valuable insights into the climate system and biotic responses to environmental variability and thus serves as an excellent analogue for future global climate changes. Here we document, for the first time, that warm and wet HWP conditions were highly favourable for magnetofossil proliferation in the semi-arid Asian interior. The pronounced increase of magnetofossil concentrations at ~9.8 ka and decrease at ~5.9 ka in Dali Lake coincided respectively with the onset and termination of the HWP, and are respectively linked to increased nutrient supply due to postglacial warming and poor nutrition due to drying at ~6 ka in the Asian interior. The two-stage transition at ~7.7 ka correlates well with increased organic carbon in middle HWP and suggests that improved climate conditions, leading to high quality nutrient influx, fostered magnetofossil proliferation. Our findings represent an excellent lake record in which magnetofossil abundance is, through nutrient availability, controlled by insolation driven climate changes.


Assuntos
Mudança Climática , Clima , Ásia , Geografia
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