Assuntos
Testes Farmacogenômicos/estatística & dados numéricos , China , Clopidogrel/metabolismo , Clopidogrel/farmacologia , Citocromo P-450 CYP2C19/efeitos dos fármacos , Citocromo P-450 CYP2C19/genética , Citocromo P-450 CYP2C9/efeitos dos fármacos , Citocromo P-450 CYP2C9/genética , Humanos , Testes Farmacogenômicos/métodos , Medicina de Precisão/instrumentação , Medicina de Precisão/métodos , Medicina de Precisão/tendências , Vitamina K Epóxido Redutases/efeitos dos fármacos , Vitamina K Epóxido Redutases/genética , Varfarina/metabolismo , Varfarina/farmacologiaRESUMO
BACKGROUND: Tyrosine (Tyr), Tryptophan (Trp) and 5-hydroxytryptamine (5-HT) are important amino acids in vivo and have been hypothesized to be involved in many mental disorders. We developed a rapid and sensitive HPLC method for simultaneous measurement of serum Tyr, Trp and 5-HT and explored the clinical significances of Tyr, Trp and 5-HT and the 5-HT/Trp ratio for patients with major depressive disorder (MDD) disease. METHODS: Serum samples were deproteinized by 5% perchloric acid and separated on an Atlantis C18 column (4.6 × 150 mm, 5 µm) with the mobile phase consisting of 0.1 mol/l KH(2)PO(4) and methanol (85:15, V/V).The eluates were monitored by the fluorescence detection with programmed wavelength. RESULTS: Analysis was achieved in <12.0 min. The limits of quantification were 0.014, 0.005, and 0.024 µmol/l for Tyr, Trp and 5-HT, respectively. Reproducibility and recovery were satisfactory. Tyr, Trp and 5-HT and the 5-HT/Trp ratio were significantly decreased in patients with MDD. CONCLUSIONS: In diseases, like MDD, Tyr, Trp and 5-HT play an important role. This method can potentially be applied as prognostic or diagnostic tool or even to follow the evolution of the illness or of the treatment.
