RESUMO
Ultra-performance liquid chromatography (UPLC), as a development of the chromatographic technique, was used in the quality control of Panax ginseng C.A. Mey. In the study, the UPLC methods were transferred from conventional high-performance liquid chromatography (HPLC) methods according to chromatographic equations. The results demonstrated that UPLC analysis methods could be transferred from HPLC without loss of efficiency. Compared with conventional HPLC, UPLC made a surprising 10-fold increase in speed and 20-fold decrease in solvent consumption. The study indicated UPLC as a suitable alternative to HPLC and can be reliably applied to quality control of traditional Chinese medicines.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/análise , Ginsenosídeos/análise , Panax/química , Cromatografia Líquida de Alta Pressão/economia , Controle de Qualidade , Sensibilidade e EspecificidadeRESUMO
OBJECTIVE: To study the chemical constituents of the flowers of Chrysanthemum indicum. METHODS: Chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures were identified by means of physico-chemical and spectral data. RESULTS: From the 80% ethanol extract of the material, seven compounds were isolated. Their structures were identified as acacetin(1), acacetin-7-O-(6"-O-acetyl) beta-D-glucopyranoside (2), linarin (3), apigenin-7-O-beta-D-glucopyranoside(4), chlorogenic acid (5), vanillic acid(6) and sucrose(7), respectively. CONCLUSION: Compounds 2, 4, 6 and 7 are isolated from C. indicum for the first time.
