Identification of a rare D13S317-5 allele in an ethnic Han Chinese from Hainan / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To report on a rare D13S317-off-ladder (OL) allele identified in an ethnic Han Chinese from Hainan.</p><p><b>METHODS</b>The rare allele D13S317-OL was detected with a short tandem repeat (STR)-PCR method and DNA sequencing.</p><p><b>RESULTS</b>The core repeat sequence of the rare allele was found to be [TATC]5.</p><p><b>CONCLUSION</b>The rare D13S317-5 allele also exists among ethnic Hans from Hainan, which may have a significant value for personal identification and paternity testing.</p>

GC-MS Analysis of the Liposoluble Constituents Extracted with Supercritical CO_2 Fluid Extraction from Aractylodes Mcrocephala / 中国药房

OBJECTIVE:To analysis and identify the liposoluble constituents extracted with supercritical CO 2 fluid ex-traction(SFE)from atractylodes macrocephala by gas chromatography-mass spectrometry(GC/MS).METHODS:Volatile oil was extracted from atractylodes macrocephala,to analsis and identify the chemical components in liposoluble constituents with GC/MS,DB-5MS capillary column was the chromatographic column and helium was carrier gas,the flow rate was 2.0ml/min,and the column temperature was raised from 70℃ to 250℃ at an increasing rate of 10℃/min.EI ion sources were adopted in mass spectra.RESULTS:A total of 19 chromatographic peaks were separated and identified,most of which were unsatu-rated fatty acids and esters,accounting for 96.2% of the total peak area.CONCLUSION:Using GC/MS,the chemical com-position of the liposoluble constituents in atractylodes macrocephala can be analyzed,which provides references for the further exploitation and utilization of atractylodes macrocephala.