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At present, it is still a challenge to prepare multifunctional composite nanomaterials with simple composition and favorable structure. Here, multifunctional Fe3O4@nitrogen-doped carbon (N-C) nanocomposites with hollow porous core-shell structure and significant electrochemical, adsorption and sensing performances were successfully synthesized through the hydrothermal method, polymer coating, then thermal annealing process in nitrogen (N2) and lastly etching in hydrochloric acid (HCl). The morphologies and properties of the as-obtained Fe3O4@N-C nanocomposites were markedly affected by the etching time of HCl. When the Fe3O4@N-C nanocomposites after etching for 30 min (Fe3O4@N-C-3) were applied as the anodes for lithium-ion batteries (LIBs), the invertible capacity could reach 1772 mA h g-1 after 100 cycles at the current density of 0.2 A g-1, which is much better than that of Fe3O4@N-C nanocomposites etched, respectively, for 15 min and 45 min (948 mA h g-1 and 1127 mA h g-1). Additionally, the hollow porous Fe3O4@N-C-3 nanocomposites also exhibited superior rate capacity (950 mA h g-1 at 0.6 A g-1). The excellent electrochemical properties of Fe3O4@N-C nanocomposites are attributed to their distinctive hollow porous core-shell structure and appropriate N-doped carbon coating, which could provide high-efficiency transmission channels for ions/electrons, improve the structural stability and accommodate the volume variation in the repeated Li insertion/extraction procedure. In addition, the Fe3O4@N-C nanocomposites etched by HCl for different lengths of time, especially Fe3O4@N-C-3 nanocomposites, also show good performance as adsorbents for the removal of the organic dye (methyl orange, MO) and surface-enhanced Raman scattering (SERS) substrates for the determination of a pesticide (thiram). This work provides reference for the design and preparation of multifunctional materials with peculiar pore structure and uncomplicated composition.
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Lítio , Nanocompostos , Porosidade , Análise Espectral Raman , Eletrodos , Carbono , Ácido Clorídrico , Íons , NitrogênioRESUMO
Multimodal combined therapy (MCT) is an emerging avenue to eliminate tumor cells by the synergistic effect of various therapeutic methods. However, the complex tumor microenvironment (TME) is becoming the key barrier to the therapeutic effect of MCT due to the excessive existence of H+ ions, H2O2, and glutathione (GSH), the lack of O2, and the relaxation of ferroptosis. To overcome these limitations, smart nanohybrid gels with excellent biocompatibility, stability and targeting function were prepared by using gold nanoclusters as cores and an in situ cross-linking composite gel of sodium alginate (SA)/hyaluronic acid (HA) as the shell. The obtained Au NCs-Cu2+@SA-HA core-shell nanohybrid gels possessed near-infrared light response synergistically benefitting photothermal imaging guided photothermal therapy (PTT) and photodynamic therapy (PDT). Meanwhile, the H+-triggered release of Cu2+ ions from the nanohybrid gels not only induces cuproptosis to avoid the relaxation of ferroptosis, but also catalyzes H2O2 in the TME to generate O2 to simultaneously improve the hypoxic microenvironment and PDT effect. Furthermore, the released Cu2+ ions could consume the excessive GSH to form Cu+ ions effectively, which caused the formation of hydroxyl free radicals (·OH) to kill tumor cells, synergistically realizing GSH consumption-enhanced PDT and chemodynamic therapy (CDT). Hence, the novel design in our work provides another research avenue for cuproptosis-enhanced PTT/PDT/CDT via TME modulation.
Assuntos
Apoptose , Nanopartículas , Neoplasias , Fotoquimioterapia , Humanos , Alginatos , Linhagem Celular Tumoral , Géis , Glutationa , Ácido Hialurônico , Peróxido de Hidrogênio , Terapia Fototérmica , Microambiente Tumoral , CobreRESUMO
With the growing use of various pesticides, it is important to develop facile and sensitive method to detect pesticides residues in food. Here, a semiconductor/magnetic hybrid material was used as surface enhanced Raman scattering (SERS) substrate to detect simulated residues. The representative sample of porous ZnO/Co3O4 nano-cube was fabricated by pyrolysis and calcination of Zn-Co ZIF, successively. The obtained hybrid of ZnO/Co3O4 was employed as substrate to detect of crystal violet (CV) and Rhodamine B (Rh B), and showed remarkable SERS performance. The detection limit of Rh B was 1 × 10-10 M as well as CV of 1 × 10-9 M. The results indicated that it was an ideal choice to improve the SERS property of transition metal oxide substrates by doping semiconductor. The semiconductor/magnetic hybrid material highlighted the obvious characteristics of low cost, facile preparation and ultra-low detection limit in the SERS measurements. The hybrids with the combination of semiconductor/magnetic properties showed a further widely application and development in SERS detection of pesticides residues.
Assuntos
Nanopartículas Metálicas , Resíduos de Praguicidas , Praguicidas , Óxido de Zinco , Análise Espectral Raman/métodos , Prata/química , Óxido de Zinco/química , Nanopartículas Metálicas/química , Porosidade , Violeta Genciana/análiseRESUMO
The complex physiological environment and inherent self-healing function of tumors make it difficult to eliminate malignant tumors by single therapy. In order to enhance the efficacy of antitumor therapy, it is significant and challenging to realize multi-mode combination therapy by utilizing/improving the adverse factors of the tumor microenvironment (TME). In this study, a novel Fe3O4@Au/PPy nanoplatform loaded with a chemotherapy drug (DOX) and responsive to TME, near-infrared (NIR) laser and magnetic field was designed for the combination enhancement of eliminating the tumor. The Fe2+ released at the low pH in TME can react with endogenous H2O2 to induce toxic hydroxyl radicals (·OH) for chemodynamic therapy (CDT). At the same time, the generated Fe3+ could deplete overexpressed glutathione (GSH) at the tumor site to prevent reactive oxygen species (ROS) from being restored while producing Fe2+ for CDT. The designed Fe3O4@Au/PPy nanoplatform had high photothermal (PT) conversion efficiency and photodynamic therapy (PDT) performance under NIR light excitation, which can promote CDT efficiency and produce more toxic ROS. To maximize the cancer-killing efficiency, the nanoplatform can be successfully loaded with the chemotherapeutic drug DOX, which can be efficiently released under NIR excitation and induction of slight acidity at the tumor site. In addition, the nanoplatform also possessed high saturation magnetization (20 emu/g), indicating a potential magnetic targeting function. In vivo and in vitro results identified that the Fe3O4@Au/PPy-DOX nanoplatform had good biocompatibility and magnetic-targeted synergetic CDT/PDT/PTT/chemotherapy antitumor effects, which were much better than those of the corresponding mono/bi/tri-therapies. This work provides a new approach for designing intelligent TME-mediated nanoplatforms for synergistically enhancing tumor therapy.
Assuntos
Nanopartículas , Neoplasias , Linhagem Celular Tumoral , Sistemas de Liberação de Medicamentos/métodos , Glutationa , Humanos , Peróxido de Hidrogênio , Nanopartículas/uso terapêutico , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Espécies Reativas de Oxigênio , Microambiente TumoralRESUMO
Exploring and developing a new type of nanoplatform with diagnosis and treatment to effectively cure tumors and reduce side effects has become a hot spot for researchers and is of great significance. Herein, a cancer theranostic nanoplatform with dual-imaging, dual-phototherapy and laser-responsiveness to tumor microenvironment was successfully assembled by liposome (Lip) co-loaded with oil-soluble Au4Cu4 nanoclusters (NCs) and water-soluble Au25 NCs via a simple film hydration method and subsequent extraction process. The prepared Au4Cu4/Au25@Lip nanoplatform with core-shell structure and about 50 nm of uniform sphere shape presented highly biocompatible, stability and passive targeting due to the enhanced permeability and retention (EPR) effect. Furthermore, the Lip composed of lecithin and cholesterol has good affinity with the cell membrane, which can realize the effective accumulation of photosensitizers at the tumor site, so that improving phototherapy effect and reducing the damage to normal tissue. The loaded oil-soluble Au4Cu4 NCs were firstly and pleasantly surprised to find possessed not only ideal photodynamic effect, but also preferable catalysis towards endogenous hydrogen peroxide (H2O2) decomposition to produce oxygen (O2) for improving the tumor hypoxic environment besides the excellent photoluminescence ability while the water-soluble Au25 NCs own outstanding photothermogenesis effect and also photoluminescence performance. The in vitro and in vivo experiment results proved that in the Au4Cu4/Au25@Lip nanoplatform, the performances of both NCs were complementary, which presenting considerable photothermal/fluorescence imaging (PTI/FI)-guided synergistic photothermal therapy (PTT)/O2-enhanced photodynamic therapy (PDT) effect for the tumor under the irradiation of near infrared (NIR) laser. This work provides a useful inspiration and paves a new way for the assembly of NCs or namomaterials with different properties into an integrated anti-tumor theranostic nanoplatform.
Assuntos
Compostos de Ouro/farmacologia , Nanopartículas Metálicas/química , Fármacos Fotossensibilizantes/farmacologia , Nanomedicina Teranóstica , Sobrevivência Celular/efeitos dos fármacos , Compostos de Ouro/química , Células HeLa , Humanos , Oxigênio , Fotoquimioterapia , Fármacos Fotossensibilizantes/química , Fototerapia , Espécies Reativas de Oxigênio , Microambiente TumoralRESUMO
Cancer is still a major threat to human health at present. Developing new types of integrated nanoplatforms for the accurate diagnosis and effective treatment of cancer is very significant. Herein, an intelligent dual-stage core-shell cancer theranostic nanoplatform (Fe3+@Au1Ag24@PbP) with NIR laser/tumor-microenvironment (TME) co-responsiveness and multi-modal imaging-therapy was successfully prepared, which was composed of the precisely structured oil-soluble Au1Ag24 nanoclusters (NCs) and Fe3+ ions easily assembled within the oil and aqueous phases of the polyethylene glycol (PEG) block grafted polyketal (PK) copolymer (PK-b-PEG, PbP) vesicles, respectively. In this system, we were delighted to find that the prepared Au1Ag24 NCs possess multi-photoresponsive properties, endowing the nanoplatform with photoacoustic (PA)/photothermal (PT) imaging and synergetic photothermal therapy (PTT)/photodynamic therapy (PDT) for cancer under near-infrared (NIR) laser irradiation. On the other hand, Fe3+ ions exhibit multi-TME response and regulation behaviors, including as catalysts for the decomposition of endogenous hydrogen peroxide (H2O2) in the solid tumor to produce O2 and as the oxidizing agent for the consumption of the intracellular GSH to avoid the reduction of the generated 1O2; therefore, the synchronously formed Fe2+ ions from the redox of Fe3+ with GSH could further react with H2O2 to produce hydroxyl radical (ËOH), which induced ferroptosis-based cancer treatment. The PbP shell possesses TME/pH sensitivity for controlled drug release and passive targeting, causing a large increase in Au1Ag24/Fe3+ accumulation within the weakly acidic tumor region and reducing the side effects on normal tissues. Both in vitro and in vivo experiments demonstrate that the Fe3+@Au1Ag24@PbP nanoplatform presented excellent PA/PT imaging-guided synergetic PTT/PDT/ferroptosis effects toward tumor cells and tumors. This integrating multi-responsive and multi-modal theranostic nanoplatform paves a new way for effective cancer therapy.
Assuntos
Nanopartículas , Neoplasias , Fotoquimioterapia , Linhagem Celular Tumoral , Humanos , Peróxido de Hidrogênio , Lasers , Imagem Multimodal , Fototerapia , Medicina de Precisão , Nanomedicina TeranósticaRESUMO
At present, cancer has become a major disease threatening human health worldwide. Therefore, developing targeting guided multimode synergetic therapy has become one of the hot spots in current antitumor research and is also a great challenge. Herein, a new Fe3O4/g-C3N4@PPy-DOX nanocomposite containing magnetic iron oxide (Fe3O4) nanoparticles (NPs), lamellar structure of graphite-like carbon nitride (g-C3N4) and polypyrrole (PPy) shell with the loaded anti-tumor drug doxorubicin hydrochloride (DOX) was designed and prepared. The monodisperse Fe3O4 nanoparticles (NPs) with the diameter of 20 nm endowed the nanocomposite with the magnetic targeting ability, reducing damage to normal tissues. It is very interesting that the Fe3O4 NPs also possessed photosensitizer function for photodynamic therapy (PDT). The g-C3N4 sheets as the photocatalysis towards the degradation of water for generating O2 could effectively improve the hypoxia of solid tumors and increase the efficiency of PDT. In addition, PPy has high light-to-heat conversion efficiency, so was chosen for the cancer photothermal therapy (PTT). Finally, an anticancer drug (DOX) was loaded on the nanocomposite because the presence of mesoporous structure. Thus, the prepared Fe3O4/g-C3N4@PPy-DOX nanocomposites exhibit synergetic chemotherapy/PTT/enhanced PDT antitumor effect. This study provides an inspiration for combining targeting and multimodality to improve the anticancer efficiency.
Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Doxorrubicina/farmacologia , Grafite/química , Nanopartículas Magnéticas de Óxido de Ferro/química , Nanocompostos/química , Neoplasias/tratamento farmacológico , Compostos de Nitrogênio/química , Polímeros/química , Pirróis/química , Protocolos de Quimioterapia Combinada Antineoplásica/química , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/química , Células Hep G2 , Humanos , Hipertermia Induzida/métodos , Hipóxia/metabolismo , Neoplasias/metabolismo , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/química , Fototerapia/métodosRESUMO
Theranostics including imaging and therapy has become an advanced trend for cancer treatment. It is of great significance to develop new theranostics agent for precision medicine research. In this regard, structurally precise metal nanoclusters (NCs) with ultra-small diameter and potential theranostic functions are no doubt to be a priority. In this study, we firstly investigated the photothermal and photodynamic properties of structurally precise oil-soluble bimetallic NCs (Pt1Ag28) and tactfully solubilize them into the hydrophobic inner cavity of biodegradable amphiphilic chitosan derivative (ACD) micelles via hydrophobic interaction. Positively charged Pt1Ag28@ACD with suitable particle size (â¼60 nm) could not only realize effectively passive targeted delivery, but also be easily uptaken by negatively charged cancer cells for increasing targeting effect. Further, the aggregation induced emission fluorescence imaging (AIE FI)-guided enhanced photothermal/photodynamic therapy (PTT/PDT) on tumor is finally nicely achieved by the micelle solubilization of ACD micelles to Pt1Ag28 NCs, which were demonstrated by in vitro and in vivo experimental results. This theranostic platform overcomes the difficulty of lipophilic NCs entering water-phase organisms, provides enlightenment for the universal study of the interaction mechanism between organisms and oil-soluble NCs at the atomic level in future precision medicine research.
Assuntos
Neoplasias , Fotoquimioterapia , Linhagem Celular Tumoral , Humanos , Micelas , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Imagem Óptica , Medicina de Precisão , Prata/uso terapêutico , Nanomedicina TeranósticaRESUMO
The photothermal and photodynamic performances of structurally precise oil-soluble AgxAu25-x (x ≤ 13) nanoclusters were first explored and they were solubilized into new assemblies to form a versatile cancer theranostic platform with tri-targeting/in situ O2-generation/aggregation enhanced fluorescence imaging/photothermal-photodynamic therapy effects, which will provide an important reference for precision theranostics at the atomic level in future.
Assuntos
Ouro/química , Nanopartículas/química , Prata/química , Oxigênio Singlete/metabolismo , Nanomedicina Teranóstica , Aminas/química , Animais , Linhagem Celular Tumoral , Compostos Férricos/química , Ácido Fólico/química , Humanos , Luz , Camundongos , Nanopartículas/uso terapêutico , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Neoplasias/terapia , Óleos/química , Imagem Óptica , Fotoquimioterapia , Fototerapia , Transplante HeterólogoRESUMO
A novel hydrogel shell on cancer cells was prepared via in situ photopolymerization of polyethyleneglycol diacrylate (PEGDA) using methylene blue (MB) sensitized mesoporous titania nanocrystal for effective photodynamic therapy (PDT). TiO2 in this system served as an effective photosensitizer and initiator for the formation of hydrogel, also can protect the MB from being degraded into an inactive form. While MB was used as a significant photosensitive additive to improve the photochemistry effects of TiO2 and widen its optical response area to near infrared region (660-900â¯nm). The PEGDA hydrogel with peritumoral injectability shows competitive advantages, i.e. good biocompatibility and hydrophily, selective and minimally invasive delivery of TiO2@MB, maintaining a high PDT drug concentration for inducing tumor apoptosis, localized and sustainable release under 660â¯nm laser irradiation for reducing side effect. The hydrogel also could load chemo-therapy drugs, and serve as a versatile platform for multimode antitumor treatment.
Assuntos
Hidrogéis/química , Azul de Metileno/farmacologia , Nanopartículas/química , Fotoquimioterapia , Polímeros/química , Titânio/química , Sobrevivência Celular/efeitos dos fármacos , Fluorescência , Células HeLa , Humanos , Nanopartículas/ultraestrutura , Polietilenoglicóis/química , Porosidade , Oxigênio Singlete/análiseRESUMO
Herein, an ordinary and mass-production approach is reported to synthesize boron (B) and nitrogen (N) co-doped three-dimensional (3D) carbon aerogels (CA) by using glucose and borax as the raw materials by a simple hydrothermal method and then carbonization in NH3 atmosphere. The porous material (BN-CA-900) possesses a large specific surface area (1032â m2 g-1 ) and high contents of doped pyridinic N and graphitic N. The onset potential (0.91â V vs. reversible hydrogen electrode, RHE), half-wave potential (0.77â V vs. RHE), and current density (5.70â mA cm-2 at 0.2â V vs. RHE) of BN-CA-900 for ORR are similar to those of commercial Pt/C, indicating that BN-CA-900 has a comparable catalytic activity with Pt/C in alkaline media. The number of electron transfer is 3.86-3.99 and the yield of hydrogen peroxide is less than 6.8 %. BN-CA-900 also presents decent catalytic performance in acidic medium. Moreover, the stability and methanol tolerance of BN-CA-900 are superior to commercial Pt/C in both alkaline and acidic media. The prepared BN-CA-900 is a promising candidate that may be applied in other areas, such as the adsorption of pollution, porous conductive electrodes, and lithium-ion batteries.
RESUMO
In this paper, we developed cysteamine-modified surface-enhanced Raman scattering (SERS) substrate for detecting detect trace amount of acidic pigment that shows weak affinity with gold nanoparticles (Au NPs). To realize sensitive and reproducible detection of pigment with weak affinity, the SERS substrate was prepared by attaching cysteamine (CA) to the Au NPs, the acidic pigment molecule could rapidly reached to the surface of Au NPs because of the formation of multihydrogen-bond and electrostatic interaction between the pigment and CA molecule. The proposed method allowed us to detect five kinds of acidic pigment with a limit of 1.0â¯ppm, which is below the strictest safety limit. Compared with the previous methods, the advantages of the present substrate were its simple substrate preparation, high reproducibility and good universality. Furthermore, the reliable and enough accurate results had been obtained by using of the proposed substrates in the assay of trace pigment in real samples.
RESUMO
A novel integrated system composed of hydrosoluble gold nanoclusters (AuNCs), two-level mesoporous canal silica, gelatin and folic acid was for the first time designed and synthesized, which could conquer poor stability of AuNCs and shows aggregation-enhanced fluorescence imaging, cancer cell-specific targeting, and synergistic photothermal/photodynamic therapies for the goal of personalized nanotheranostics.
Assuntos
Antineoplásicos/química , Ouro/química , Nanopartículas/química , Imagem Óptica , Fotoquimioterapia , Dióxido de Silício/química , Animais , Antineoplásicos/farmacologia , Células CHO , Sobrevivência Celular/efeitos dos fármacos , Cricetulus , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Células MCF-7 , Tamanho da Partícula , Porosidade , Solubilidade , Relação Estrutura-Atividade , Propriedades de Superfície , Nanomedicina TeranósticaRESUMO
A novel cabbage-like Fe3O4@MoS2@ZnO nanocomposite was successfully fabricated through a facile method. The as-prepared nanocomposite exhibited a saturation magnetization of 45 emu g-1 as well as possessed a massive pore structure and large surface area, leading to a high DOX loading capacity of 68.14 µg mg-1; it could effectively deliver drugs to tumor lesion sites under the action of magnetic targeting. The pH-dependent ZnO as a packaging component can block the pores to achieve controlled release of DOX under tumor stimulation conditions (pH 6.5), thereby reducing the side effects of DOX on normal cells and increasing its therapy effects on tumor cells. Moreover, the photothermal conversion efficiency contributed by MoS2 under 808 nm NIR laser irradiation was utilized to realize effective photothermal therapy (PTT) of cancer, which could be integrated with chemotherapy in a single system. Thus, the resulting Fe3O4@MoS2@ZnO nanocomposites provide hopeful prospects in biomedical applications based on pH sensitivity, magnetic targeting and chemo-photothermal synergistic therapy.
RESUMO
Polyvinyl alcohol (PVA) micelles were firstly synthesized by using hemin molecules as novel crosslinked bridges (PVA-H crosslinked micelles). On one hand, the crosslinked micelles can allow high stability against extensive dilution (1800-fold) to reduce side-effects; On the other hand, the bridges not only can destruct by means of laser irradiation (405nm, 200mw), but also can induce the production of singlet oxygen (1O2) and the release of 5-fluorouracil (5FU) (i.e. 46% release in 360min). But above all, that 5FU-loaded PVA-H crosslinked micelles irradiating by laser is more efficient than PVA-H crosslinked micelles and free 5FU in killing tumor cells, suggest the effective synergistic antitumor effect about chemotherapy and photodynamic therapy (PDT).
Assuntos
Álcool de Polivinil/química , Fluoruracila , Micelas , Fotoquimioterapia , Cloreto de PolivinilaRESUMO
In this study, the poly (ethylene glycol) double acrylates (PEGDA) was first initiated by Spinacia oleracea L. extract (SOLE) to in situ form a polymer hydrogel shell on Hela cells with near infra-red irradiation. The SOLE was also a natural photosensitizer for photodynamic therapy of tumor. The use of SOLE is simple, eco-friendly, low cost, and convenient. More importantly, the PEGDA/SOLE composite hydrogel shell on Hela cells not merely prevents SOLE from diffusing to normal tissue for reducing the side effects, but also keeps high SOLE concentration on tumor cell for improving the antitumor effect. Such a hydrogel system has good biocompatibility and exhibits high therapeutic efficacy. This study extended the application of the SOLE/PEGDA gel in photodynamic therapy.
Assuntos
Hidrogéis , Neoplasias/tratamento farmacológico , Fotoquimioterapia/métodos , Extratos Vegetais , Polietilenoglicóis , Spinacia oleracea/química , Células HeLa , Humanos , Hidrogéis/química , Hidrogéis/farmacologia , Neoplasias/metabolismo , Neoplasias/patologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Polietilenoglicóis/química , Polietilenoglicóis/farmacologiaRESUMO
A reduced graphene oxide (RGO)/gold nanorod (AuNR)/hydroxyapatite (HA) nanocomposite was designed and successfully synthesized for the first time. An anticancer drug, 5-fluorouracil (5FU), was chosen as a model drug to be loaded in RGO/AuNR/HA. The fabricated RGO/AuNR/HA-5FU showed robust, selective targeting and penetrating efficiency against HeLa cells due to the good compatibility and nontoxicity of HA, and showed excellent synergetic antitumor effects through combined chemotherapy (CT) by 5FU and photothermal therapy (PTT) by both RGO and AuNRs under near-infrared (NIR) laser irradiation. More importantly, this synergistic dual therapy based on RGO/AuNR/HA can also minimize side effects in normal cells and exhibits greater antitumor activity because of a multi-stage drug release ability triggered by the pH sensitivity of HA in the first stage and the combined photothermal conversion capabilities of RGO and AuNRs by means of the NIR laser irradiation in the second stage. This study suggests that the novel RGO/AuNR/HA multi-stage drug delivery system may represent a promising potential application of multifunctional composite materials in the biomedical field.
Assuntos
Antimetabólitos Antineoplásicos/uso terapêutico , Fluoruracila/uso terapêutico , Ouro/uso terapêutico , Grafite/uso terapêutico , Nanocompostos/uso terapêutico , Neoplasias/terapia , Antimetabólitos Antineoplásicos/administração & dosagem , Terapia Combinada/métodos , Sistemas de Liberação de Medicamentos/métodos , Durapatita/química , Durapatita/uso terapêutico , Fluoruracila/administração & dosagem , Ouro/química , Grafite/química , Células HeLa , Humanos , Nanocompostos/química , Nanotubos/química , Nanotubos/ultraestrutura , Oxirredução , Óxidos/química , Óxidos/uso terapêutico , Fototerapia/métodosRESUMO
In this study, graphene oxide and creatine phosphate disodium acted as dual template and was employed to synthesize graphene oxide (GO)/hydroxyapatite (HA) hybrids as drug carriers. In the rapid preparation of GO/HA hybrids, creatine phosphate disodium salt (CPDS) severed as a phosphorus source and graphene oxide acted as a template in aqueous solution. The effects of the reaction temperature, time and pH value of the aqueous solution on the morphology of the product were investigated. The result showed that the hydrolysis of CPDS under hydrothermal condition played an important role in the formation of hierarchical hollow GO/HA hybrids. The GO nanosheets provided reactive sites for the binding of HA nanoparticles and absorbing ibuprofen (IBU) molecules. The GO/HA hybrids had ideal sustained drug-release behavior. It indicated that the prepared GO/HA hybrids may be promising materials for applications in biomedical area.
Assuntos
Sistemas de Liberação de Medicamentos/métodos , Durapatita/síntese química , Grafite/síntese química , Fosfocreatina/síntese química , Adsorção , Durapatita/química , Grafite/química , Ibuprofeno/farmacologia , Microscopia Eletrônica de Varredura , Fosfocreatina/química , Porosidade , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Difração de Raios XRESUMO
A novel litchi-like porous composite composed of a magnetic core, a tunable metal-organic framework (MOF) shell and a pH-sensitive hydroxyapatite (HAp) gatekeeper was successfully fabricated in this work. The anticancer drug doxorubicin (DOX) was effectively loaded on the Fe3O4@Fe-MOF@HAp nanocomposite with a loading capacity of 75.38 mg g-1. The nanocomposite had a saturation magnetization of 34 emu g-1 and thus possessed magnetic targeting function. In addition, the HAp gate, which had favorable biocompatibility and pH response characteristics, could be used to control the release of loaded DOX from the Fe3O4@Fe-MOF@HAp nanocomposite microspheres in a simulated acidic tumor cell environment, effectively killing tumor cells and reducing the toxic side effects to normal tissue. The smart design presented in this study, which incorporates a tunable shell and gate-controlled architecture, allows the sensitive release of drugs for efficient antitumor activity.
RESUMO
A novel porous graphene oxide (GO)/chitosan (CTS)-hydroxyapatite (HA) nanocomposite film was successfully prepared for the first time by combining layer-by-layer (LBL) assembly technology with biomimetic mineralization method. The LBL technology was used to control the thickness of film as well as induce the biomimetic mineralization of biocompatible HA. The obtained (GO/CTS-HA)n film provided ideal platform for the proliferation of mouse mesenchymal stem cells (mMSCs). The pore size in the film is about 300nm, and the porous architecture made the film have high aspirin loading efficiency. Also the accumulated loading dosage could be adjusted by the film thickness, and the sustained release of aspirin could ensure well anti-inflammatory effect. The above advantages may alleviate the pain of patients and give the better environment for bone regeneration. This multifunctional aspirin-loaded (GO/CTS-HA)n film provided an inspiration for the synthesis of novel porous inorganic/biomacromolecule nanocomposite films as the biocoatings applied in bone tissue engineering.