RESUMO
OBJECTIVE: To select and obtain the best evidence for parastomal hernia (PH) prevention in patients with enterostomy so as to provide reference for clinical practice to decrease the rate of PH. METHODS: Based on the method of evidence-based nursing, this paper proposes the prevention and management of PH in patients with enterostomy. The literature was checked according to the "6S" model, and literature evaluation standards (2016 edition) of JBI Evidence-based Health Care Center in Australia were adopted to evaluate the literature quality and evidence level of various studies. RESULTS: Combined with the judgment of professionals, 24 pieces of relevant evidence from 7 dimensions were summarized, including related controllable risk factors, diagnosis and identification, nutrition, the strength of abdominal wall muscle around the stoma, reduction of abdominal pressure, the use of belt on treatment of PH, and the prevention of complications related to PH. CONCLUSIONS: This study summarized the best evidence of nonsurgical prevention and management of PH in patients with enterostomy, and provided an evidence-based basis for nurses to carry out clinical work, so as to use scientific methods to manage and prevent the occurrence of PH in patients with enterostomy and improve the quality of care.
RESUMO
A new and simple method for wounding cotyledonary node cells of soybean [Glycine max (L) Merrill] was developed for obtaining a high frequency of transformants. Soybean seeds were germinated for 1 day, and the cotyledonary node cells of half-seeds were wounded mechanically by using a multi-needle consisting of thin 30 fibers. The wounded half-seeds were inoculated with Agrobacterium tumefaciens cells harboring a recombinant DNA that contained the bar and sgfp genes conferring phosphinothricin (PPT)-resistance and green fluorescent protein (GFP) activity, respectively. The inoculated explants were selected on medium containing 5 or 3 mg PPT/l. The transformation efficiency of soybean was up to 12%. Polymerase chain reaction and genomic Southern blot analysis confirmed stable integration of the transgenes in the genome of the PPT-resistant plants. GFP analysis revealed that the transgenes were highly expressed in the plantlets. Adult plants were resistant to 100 mg PPT/l applied on the leaves, demonstrating their herbicide-resistance.
