Study on development methods of different types of gas wells in tight sandstone gas reservoirs.

Reasonable production allocation of tight sandstone gas reservoirs is an important basis for efficient development of gas wells. Taking Block XX in Ordos Basin as an example, the modified flowing material balance equation was established considering the variation of gas viscosity and compression coefficient, the advantages and disadvantages of the method were discussed, and a reasonable production allocation process for gas wells was developed. The results show that: ① The commonly used flow material balance method ignores the change of natural gas compression coefficient, viscosity and deviation coefficient in the production process. The slope of the relationship curve between bottom hole pressure and cumulative production and the slope of the relationship curve between average formation pressure and cumulative production are not equal After considering this change. Compared with the results calculated by the material balance method, the results calculated by the flow material balance method are smaller. ② The production of 660 gas wells in the study area during stable production period is verified. Compared with the open flow method, the dynamic reserve allocation method is better, with an error of 0.06%. ③ The new method in this paper is used to allocate production for different types of gas wells. The cumulative production of different types of gas wells shows different degrees of increase. The I, II, III and IV types of gas wells increase by 32.26%, 30.29%, 23.58% and 25.07% respectively. This study provides technical support for dynamic reserve calculation and reasonable production allocation of gas wells in the study area, and has important guiding significance for the formulation of reasonable development plan and economic and efficient development of tight sandstone gas reservoirs.

A Temporin Derived Peptide Showing Antibacterial and Antibiofilm Activities against Staphylococcus aureus.

BACKGROUND: Temporin is one family of the shortest antimicrobial peptides found in Ranidae frogs. Staphylococcus aureus is one of the main pathogens of suppurative diseases and food contamination, causing severe local or systemic infections in humans. Temporin-GHa (GHa) was previously obtained from Hylarana guentheri, showing weak antibacterial activity against S. aureus. Most temporin peptides are positively charged by arginine and lysine; however, GHa contains histidine. OBJECTIVE: In order to investigate the impact of positively charged amino acid on its antibacterial and antibiofilm activity, GHa4R was designed and synthesized by replacing histidine with arginine in GHa. METHODS: The antibacterial activity and efficacy against S. aureus were detected by minimum inhibitory concentration, minimum bactericidal concentration, and time-killing kinetics assays. The action mechanism was determined by propidium iodide uptake and scanning electron microscopy assays. The antibiofilm activity was measured by the MTT method. Eradication of biofilm was observed by fluorescence microscope. RESULTS: Compared to GHa, GHa4R had stronger antibacterial activity and bactericidal efficacy against S. aureus. Impressively, GHa4R presented antibacterial activity against methicillin-resistant S. aureus (MRSA). It was barely affected by temperature, pH, and storage period, showing high stability. Furthermore, it increased the permeability of the cell membrane and damaged the membrane integrity, leading to cell death. In addition, GHa4R did not induce antibiotic resistance in S. aureus in 30 days, but the MIC of vancomycin was doubled. It not only inhibited S. aureus biofilm formation but also eradicated 24 h-biofilms. CONCLUSION: The above-mentioned characteristics make GHa4R a promising candidate for the treatment of S. aureus infections.

Four temporin-derived peptides exhibit antimicrobial and antibiofilm activities against methicillin-resistant.

Temporin-GHa (GHa) was cloned from , showing a weak antimicrobial activity. In order to improve its bactericidal efficacy, GHaR6R, GHaR7R, GHaR8R and GHaR9W were designed and synthesized. Compared to the parent peptide, the GHa-derived peptides show potent antimicrobial activities against methicillin-resistant (MRSA), which is the main pathogen with high morbidity and mortality that causes various infections in humans. These peptides exert bactericidal actions on MRSA by permeabilizing the cytoplasmic membranes and damaging membrane integrity. All of the four peptides exhibit excellent stability under harsh conditions, including extreme temperature and salts. Furthermore, they inhibit the formation of biofilm and eradicate mature biofilm of MRSA. The GHa-derived peptides decrease bacterial surface hydrophobicity, autoaggregation and polysaccharide intercellular adhesion synthesis in concentration-dependent manner. Real-time quantitative reverse transcription PCR analysis revealed that the peptides downregulate the expression of adhesion genes involved in biofilm formation. Except for GHaR7R, the other three peptides have low hemolytic toxicity against human erythrocytes. In the presence of human erythrocytes, GHaR7R, GHaR8R and GHaR9W interact with MRSA preferentially. GHaR6R, GHaR8R and GHaR9W show less toxicity toward normal cells HL-7702 and hFOB1.19. These results suggest that the GHa-derived peptides may be promising antimicrobial candidates against MRSA infections.