Long-Term Agricultural Effects on the Authentication Accuracy of Organic, Green, and Conventional Rice Using Isotopic and Elemental Chemometric Analyses.

Organically farmed rice is believed to be healthier, safer, and eco-friendlier than its conventionally farmed counterparts and sells for a premium price in global markets. Deliberate mislabeling of organic rice has become a critical consumer concern in China and elsewhere, and there is an increased risk of buying fraudulent organic rice in the market place. In this study, stable isotopic and multielemental analysis combined with chemometrics was used to differentiate organically farmed rice from green and conventional rice in a 4-year experimental field trial from 2014 to 2017. A total of 108 rice samples and their associated soils were collected during the study from three farming (fertilization) systems to investigate whether there are long-term changes in the rice farming classification accuracy from climate effects. Stable carbon and nitrogen isotopic ratios (i.e., δ13C and δ15N) and 27 elemental contents (e.g., Na, K, Ca, Fe, and Zn) of rice and soil samples were determined and then evaluated using statistical analysis [i.e., one-way analysis of variance, multivariable correlation analysis, and modeling of partial least-squares discriminant analysis]. Although δ15N values can be an effective indicator for organic rice authentication during one crop rotation, both δ13C and δ15N values of rice were easily affected by rice cultivar and interannual soil fertilization and localized agroclimatic variations. These two isotopes were not able to separate organic rice from green and conventional rice accurately. Elemental contents of green and conventional rice (especially K and Ca) were found at higher levels due to the abundant application of synthetic fertilizers (e.g., KNO3, KH2PO4, and CaHPO4), unlike organically farmed rice, which primarily used animal manure and composts. Partial least-squares discriminant analysis modeling combined isotopic and elemental signatures to correctly differentiate organic rice from green and conventional counterparts, with an accuracy up to 100% over the 4-year study. Therefore, this multi-isotope and -element strategy proposes a more rigorous, alternative tool to combat fraudulent mislabeling of organic rice, increasing the trust of organically labeled rice products and supporting the integrity of the organic sector worldwide.

Efficient Conversion of Fructose-Based Biomass into Lipids with Trichosporon fermentans Under Phosphate-Limited Conditions.

Limiting nitrogen supply has been routinely used as the master regulator to direct lipid biosynthesis. However, this strategy does not work with nitrogen-rich substrates, such as Jerusalem artichoke (JA), a fructose-based biomass, while it is difficult to obtain a high carbon-to-nitrogen (C/N) molar ratio. In this study, an alternative strategy to promote lipid accumulation by the oleaginous yeast Trichosporon fermentans CICC 1368 was developed by limiting phosphorous supply, and this strategy was implemented with JA hydrolysate as substrate. We showed that lipid accumulation was directly correlated with the C/P ratio of the culture media for T. fermentans. The time course of cell growth and lipid production was analyzed in a media with an initial C/P ratio of 6342, and the cellular lipid content could reach up to 48.5% of dry biomass. Moreover, JA hydrolysates were used as substrate for microbial lipid accumulation, under high C/P molar ratio condition, lipid yield, lipid content, and lipid coefficient increased by 10, 30, and 34%, respectively. It showed that by limiting phosphorus, the conversion of sugar into lipids can be improved effectively. Limiting phosphorus provides a promising solution to the problem of microbial lipid production with nitrogen-rich natural materials.

[Control effects of rice-duck farming and other weed management strategies on weed communities in paddy fields].

By the methods of community ecology, field studies were conducted to evaluate the control effects of three weed management strategies, i. e., rice-duck farming (RD), manual weeding (MW) and chemical weeding (CW), on the weed communities in paddy fields. The results showed that under rice-duck farming, the weed density in paddy fields decreased significantly, and the control effects on dominant weed species such as Monochoria vaginalis, Cyperus difformis, Sagittaria pygmaea were all above 95%, with an overall effect higher than CW and MW. Under RD, the species richness and Shannon-Wiener diversity indices decreased slightly, while Pielou community evenness indices increased markedly, indicating that the species composition of weed community was greatly improved, and the infestation of former dominant weed species was reduced. The structure of weed communities in paddy fields varied with different weed management strategies, e. g., under RD, Lindernia procumbens, Cyperus difformis and Fimbristylis miliacea constituted the major weed community, and the Whittaker index was significant higher than that of CW, MW and CK, which indicated that rice-duck farming had a greater effect on the structure of the weed communities. The same conclusion could be drawn from Sorensen's similarity indices and cluster analysis with Sorensen's index as the distance measurement.