Enhancement of cadmium uptake in Sedum alfredii through interactions between salicylic acid/jasmonic acid and rhizosphere microbial communities.

The focus on phytoremediation in soil cadmium (Cd) remediation is driven by its cost-effectiveness and eco-friendliness. Selecting suitable hyperaccumulators and optimizing their growth conditions are key to enhance the efficiency of heavy metal absorption and accumulation. Our research has concentrated on the role of salicylic acid (SA) and jasmonic acid (JA) in facilitating Cd phytoextraction by "Sedum alfredii (S. alfredii)" through improved soil-microbe interactions. Results showed that SA or JA significantly boosted the growth, stress resistance, and Cd extraction efficiency in S. alfredii. Moreover, these phytohormones enhanced the chemical and biochemical attributes of the rhizosphere soil, such as pH and enzyme activity, affecting soil-root interactions. High-throughput sequencing analysis has shown that Patescibacteria and Umbelopsis enhanced S. alfredii's growth and Cd extraction by modifying the bioavailability and the chemical conditions of Cd in soil. Structural Equation Model analysis further verified that phytohormones significantly enhanced the interaction between S. alfredii, soil, and microbes, leading to a marked increase in Cd accumulation in the plant. These discoveries emphasized the pivotal role of phytohormones in modulating the hyperaccumulators' response to environmental stress and offered significant scientific support for further enhancing the potential of hyperaccumulators in ecological restoration technologies using phytohormones.

Physiology and transcriptomic analysis revealed the mechanism of silicon promoting cadmium accumulation in Sedum alfredii Hance.

Silicon (Si) effectively promote the yield of many crops, mainly due to its ability to enhance plants resistance to stress. However, how Si helps hyperaccumulators to extract Cadmium (Cd) from soil has remained unclear. In this study, Sedum alfredii Hance (S. alfredii) was used as material to study how exogenous Si affected biomass, Cd accumulation, antioxidation, cell ultrastructure, subcellular distribution and changes in gene expression after Cd exposure. The study has shown that as Si concentration increases (1, 2 mM), the shoot biomass of plants increased by 33.1%-63.6%, the Cd accumulation increased by 31.9%-96.6%, and the chlorophyll, carotenoid content, photosynthetic gas exchange parameters significantly increased. Si reduced Pro and MDA, promoted the concentrations of SOD, CAT and POD to reduce antioxidant stress damage. In addition, Si promoted GSH and PC to chelate Cd in vacuoles, repaired damaged cell ultrastructure, improved the fixation of Cd and cell wall (especially in pectin), and reduced the toxic effects of Cd. Transcriptome analysis found that genes encoding Cd detoxification, Cd absorption and transport were up-regulated by Si supplying, including photosynthetic pathways (PSB, LHCB, PSA), antioxidant defense systems (CAT, APX, CSD, RBOH), cell wall biosynthesis such as pectinesterase (PME), chelation (GST, MT, NAS, GR), Cd absorption (Nramp3, Nramp5, ZNT) and Cd transport (HMA, PCR). Our result revealed the tentative mechanism of Si promotes Cd accumulation and enhances Cd tolerance in S. alfredii, and thereby provides a solid theoretical support for the practical use of Si fertilizer in phytoextraction.

Salicylic Acid's impact on Sedum alfredii growth and cadmium tolerance: Comparative physiological, transcriptomic, and metabolomic study.

With the acceleration of industrialization, Cd pollution has emerged as a major threat to soil ecosystem health and food safety. Hyperaccumulating plants like Sedum alfredii Hance are considered to be used as part of an effective strategy for the ecological remediation of Cd polluted soils. This study delved deeply into the physiological, transcriptomic, and metabolomic responses of S. alfredii under cadmium (Cd) stress when treated with exogenous salicylic acid (SA). We found that SA notably enhanced the growth of S. alfredii and thereby increased absorption and accumulation of Cd, effectively alleviating the oxidative stress caused by Cd through upregulation of the antioxidant system. Transcriptomic and metabolomic data further unveiled the influence of SA on photosynthesis, antioxidant defensive mechanisms, and metal absorption enrichment pathways. Notably, the interactions between SA and other plant hormones, especially IAA and JA, played a central role in these processes. These findings offer us a comprehensive perspective on understanding how to enhance the growth and heavy metal absorption capabilities of hyperaccumulator plants by regulating plant hormones, providing invaluable strategies for future environmental remediation efforts.

Jasmonic acid's impact on Sedum alfredii growth and cadmium tolerance: A physiological and transcriptomic study.

Soil cadmium (Cd) pollution is escalating, necessitating effective remediation strategies. This study investigated the effects of exogenous jasmonic acid (JA) on Sedum alfredii Hance under Cd stress, aiming to enhance its phytoextraction efficiency. Initially, experiments were conducted to assess the impact of various concentrations of JA added to environments with Cd concentrations of 100, 300, and 500 µmol/L. The results determined that a concentration of 1 µmol/L JA was optimal. This concentration effectively mitigated the level of ROS products by enhancing the activity of antioxidant enzymes. Additionally, JA fostered Cd absorption and accumulation, while markedly improving plant biomass and photosynthetic performance. In further experiments, treatment with 1 µmol/L JA under 300 µmol/L Cd stress was performed and transcriptomic analysis unveiled a series of differentially expressed genes (DEGs) instrumental in the JA-mediated Cd stress response. These DEGs encompass not only pathways of JA biosynthesis and signaling but also genes encoding functions that influence antioxidant systems and photosynthesis, alongside genes pertinent to cell wall synthesis, and metal chelation and transport. This study highlights that JA treatment significantly enhances S. alfredii's Cd tolerance and accumulation, offering a promising strategy for plant remediation and deepening our understanding of plant responses to heavy metal stress.

[Response of Soil Organic Carbon Accumulation in Paddy Fields in Fujian Province to Future Temperature Increases].

Understanding the effect of the soil carbon "source-sink" in cropland in China under future warming scenarios is the basis for making reasonable carbon neutralization policies. This study focused on the paddy soil in Fujian Province, a typical subtropical region in China including 84 counties (cities and districts). We employed the 1:50000 soil database and biogeochemical process model (DNDC) to simulate the dynamic changes in paddy soil organic carbon under different warming scenarios for the period of 2017-2053. The results indicated that in the context of normal temperature (control run) and 2, 4, and 6℃ of warming, the total amounts of carbon sequestration of paddy soil in Fujian Province were 11.56,9.44, 7.08, and 4.91 Tg, respectively; accordingly, the average annual carbon sequestration rates (expressed by C) were 173, 141, 106, and 74 kg·(hm2·a)-1, indicating that the rate of carbon sequestration was decreasing with the increase in future temperature. However, overall, the paddy field soil in the province was still a "carbon sink" under the warming of 6 (C. We also found that the gleyed paddy soil was mostly affected by the increase in temperature, and the decrease in carbon sequestration rate ranged from 20% to 69% using different treatments. In contrast, the salinized paddy soil was slightly affected, with a 14%-43% decrease in carbon sequestration rates. As for the different administrative regions, Sanming City was the most affected by temperature increase, with the rate of carbon sequestration decreasing by 27%-83% using different treatments. However, it was reduced by only 10%-41% and 14%-42% in Quanzhou and Putian (coastal areas), respectively. Overall, due to different soil properties, fertilization management, and climatic environment, there was a strong variability in the carbon sequestration rates of paddy soil for different soil subtypes and administrative regions in Fujian in response to future climatic warming.

Biochar loaded with bacteria enhanced Cd/Zn phytoextraction by facilitating plant growth and shaping rhizospheric microbial community.

Biochar and metal-tolerant bacteria have been widely used in the remediation of heavy metal contaminated soil. However, the synergistic effect of biochar-functional microbes on phytoextraction by hyperaccumulators remains unclear. In this study, the heavy metal-tolerant strain Burkholderia contaminans ZCC was selected and loaded on biochar to produce biochar-resistant bacterial material (BM), and the effects of BM on Cd/Zn phytoextraction by Sedum alfredii Hance and rhizospheric microbial community were explored. The results showed that, BM application significantly enhanced the Cd and Zn accumulation of S. alfredii by 230.13% and 381.27%, respectively. Meanwhile, BM alleviated metal toxicity of S. alfredii by reducing oxidative damage and increasing chlorophyll and antioxidant enzyme activity. High-throughput sequencing revealed that BM significantly improved soil bacterial and fungal diversity, and increased the abundance of genera with plant growth promoting and metal solubilizing functions such as Gemmatimonas, Dyella and Pseudarthrobacter. Co-occurrence network analysis showed that BM significantly increased the complexity of the rhizospheric bacterial and fungal network. Structural equation model analysis revealed that soil chemistry property, enzyme activity and microbial diversity contributed directly or indirectly to Cd and Zn extraction by S. alfredii. Overall, our results suggested that biochar- B. contaminans ZCC was able to enhance the growth and Cd/Zn accumulation by S. alfredii. This study enhanced our understanding on the hyperaccumulator-biochar-functional microbe interactions, and provided a feasible strategy for promoting the phytoextraction efficiency of heavy metal contaminated soils.

Exogenous silicon promotes cadmium (Cd) accumulation in Sedum alfredii Hance by enhancing Cd uptake and alleviating Cd toxicity.

Soil Cadmium (Cd) pollution has become a serious environmental problem. Silicon (Si) plays key roles in alleviating Cd toxicity in plants. However, the effects of Si on mitigation of Cd toxicity and accumulation of Cd by hyperaccumulators are largely unknown. This study was conducted to investigate the effect of Si on Cd accumulation and the physiological characteristics of Cd hyperaccumulator Sedum alfredii Hance under Cd stress. Results showed that, exogenous Si application promoted the biomass, Cd translocation and concentration of S. alfredii, with an increased rate of 21.74-52.17% for shoot biomass, and 412.39-621.00% for Cd accumulation. Moreover, Si alleviated Cd toxicity by: (i) increasing chlorophyll contents, (ii) improving antioxidant enzymes, (iii) enhancing cell wall components (lignin, cellulose, hemicellulose and pectin), (iv) raising the secretion of organic acids (oxalic acid, tartaric acid and L-malic acid). The RT-PCR analysis of genes that involved in Cd detoxification showed that the expression of SaNramp3, SaNramp6, SaHMA2 and SaHMA4 in roots were significantly decreased by 11.46-28.23%, 6.61-65.19%, 38.47-80.87%, 44.80-69.85% and 33.96-71.70% in the Si treatments, while Si significantly increased the expression of SaCAD. This study expanded understanding on the role of Si in phytoextraction and provided a feasible strategy for assisting phytoextraction Cd by S. alfredii. In summary, Si facilitated the Cd phytoextraction of S. alfredii by promoting plant growth and enhancing the resistance of plants to Cd.

Nitrogen retention potentials of magnesium oxide- and sepiolite-modified biochars and their impacts on bacterial distribution under nitrogen fertilization.

Mitigating the loss and negative impacts of reactive N from fertilized soils remains a global environmental challenge. To optimize N retention by biochar, bamboo and pig manure biochars were modified as MgO- and sepiolite-biochar composites and characterized. Novel soil application of the modified biochars and their raw forms were comparatively evaluated for N-retention in a fertilized soil leached for 90 days in a column experiment. Changes in N-cycling-related enzyme and bacterial structure were also reported after 90 days. Results revealed low leaching losses of NH4+, which reduced over time across all the treatments. However, while sole fertilizer (F) increased the initial and cumulative NO3- leached from the soil, the MgO-bamboo biochar (MgOBF) and sepiolite-bamboo biochar (SBF) treatments reduced leachate NO3- by 22.1 % and 10.5 % compared to raw bamboo biochar (BBF) treatment. However, 15.5 % more NO3- was leached from the MgO-pig manure biochar-treated soil (MgOPF) compared to its raw biochar treatment (PMBF) after 90 days. Dissolved organic N leached was reduced by 9.2 % and 0.5 % in MgOBF and SBF, as well as 15.4 % and 40.5 % in MgOPF and SPF compared to their respective raw forms. The total N of the biochars, adjustment of surface charges, cation exchange capacity, surface area, pore filling effects, and the formation of potential MgN precipitates on the modified-biochar surfaces regulated N leaching/retention. In addition, the modified biochar treatments reduced the hydrolysis of urea and stimulated some nitrate-reduction-related bacteria crucial for NO3- retention. Hence, unlike the raw biochar and MgOPF treatments, MgOBF, SBF, and SPF hold promise in mitigating inorganic-N losses from fertilized soils while improving the soil's chemical properties.

[Enrichment and Ecological Risk Assessment of Available Phosphorus in Paddy Soil of Fujian Province Over Past 40 years].

The ecological risks such as water eutrophication caused by soil phosphorus loss have attracted extensive attention, and its dynamic changes and enrichment effects are the basis for formulating reasonable control measures. In this study, based on the paddy soils of 1.8×106 hm2 in Fujian province, the dynamic changes and ecological risks of available phosphorus in paddy soils over the past 40 years were analyzedusing a soil database of 1:50000. The soil database contained 1471, 215534, and 2895 paddy soil samples in different periods, respectively. The paddy soil samples were derived from the 1982 Second National Soil Census, the 2008 Ministry of Agriculture and Rural Areas Soil Testing and Formulated Fertilization Project and the 2018 Ministry of Agriculture, and the Rural Areas Arable Land Quality Monitoring Project, respectively. The results showed that from 1982 to 2018, the content of available phosphorus in paddy soils increased by 47 mg·kg-1, and the enriched area reached 1.65×106 hm2, accounting for 91% of the total paddy soils in Fujian province. From 1982 to 2008, the available phosphorus content of paddy soils in Fujian province increased by 28 mg·kg-1, with the enriched area reaching 1.47×106 hm2, accounting for 82% of the total paddy soils in Fujian province. From 2008 to 2018, the available phosphorus content of paddy soils in Fujian province increased by 19 mg·kg-1, with the enriched area reaching 1.22×106 hm2, accounting for 69% of the total paddy soils in Fujian province. Further ecological risk assessment showed that from 2008 to 2018, the area of paddy soil with ecological phosphorus enrichment risk in the province gradually increased, mainly distributed in percogenic paddy soils and hydromorphic paddy soils with a slope of less than 2°. In the future, effective phosphorus fertilizer management measures should be formulated for different types of paddy soil to prevent the occurrence of environmental problems such as water eutrophication.

Wood vinegar facilitated growth and Cd/Zn phytoextraction of Sedum alfredii Hance by improving rhizosphere chemical properties and regulating bacterial community.

Soil Cd and Zn contamination has become a serious environmental problem. This work explored the performance of wood vinegar (WV) in enhancing the phytoextraction of Cd/Zn by hyperaccumulator Sedum alfredii Hance. Rhizosphere chemical properties, enzyme activities and bacterial community were analyzed to determine the mechanisms of metal accumulation in this process. Results demonstrated that, after 120 days growth, different times dilution of WV increased the shoot biomass of S. alfredii by 85.2%-148%. In addition, WV application significantly increased soil available Cd and Zn by lowing soil pH, which facilitated plant uptake. The optimal Cd and Zn phytoextraction occurred from the 100 times diluted WV (D100), which increased the Cd and Zn extraction by 188% and 164%, compared to CK. The 100 and 50 times diluted WV significantly increased soil total and available carbon, nitrogen and phosphorus, and enhancing enzyme activities of urease, acid phosphatase, invertase and protease by 10.1-21.4%, 29.1-42.7%,12.2-38.3% and 26.8-85.7%, respectively, compared to CK. High-throughput sequencing revealed that the D 100 significantly increased the bacterial diversity compared to CK. Soil bacterial compositions at phylum, family and genera level were changed by WV addition. Compared to CK, WV application increased the relative abundances of genus with plant growth promotion and metal mobilization function such as, Bacillus, Gemmatimonas, Streptomyces, Sphingomonas and Polycyclovorans, which was positively correlated to biomass, Cd/Zn concentrations and extractions by S. alfredii. Structural equation modeling analysis showed that, soil chemical properties, enzyme activities and bacterial abundance directly or indirectly contributed to the biomass promotion, Cd, and Zn extraction by S. alfredii. To sum up, WV improved phytoextraction efficiency by enhancing plant growth, Cd and Zn extraction and increasing soil nutrients, enzyme activities, and modifying bacterial community.

Field-applied biochar-based MgO and sepiolite composites possess CO2 capture potential and alter organic C mineralization and C-cycling bacterial structure in fertilized soils.

Agricultural soils contribute a significant amount of anthropogenic CO2 emission, a greenhouse gas of global environmental concern. Hence, discovering sustainable materials that can capture CO2 in cultivated soils is paramount. Since the effect of biochar on C mineralization/retention in fertilized soils is unclear, we produced biochar-based MgO and sepiolite-nanocomposites with CO2 capture potential. The field-scale impacts of the modified-biochars were evaluated on net C exchange rate (NCER) periodically for 3 months in fertilized plots. The effects of the modified-biochar on organic-C mineralization, the activities, and dynamics of C-cycling-related 16S rRNA which are unknown, were investigated. Results revealed an initial rapid and higher cumulative CO2 emission from the sole fertilizer treatment (F). Unlike the biochar treatment (BF), the successful incorporation of MgO/Mg(OH)2 nanoparticles into the matrix and surface of biochar, and the potential formation of MgCO3 with soil CO2, mitigated CO2 emission, especially in the MgO-modified biochar (MgOBF), compared to the sepiolite-biochar treatment (SBF). Compared to F and BF, the higher C retention as MgCO3 in the modified biochar treatments led to an increase in cellulase activity, stimulation of key C-cycling-related bacteria, and the expression of genes associated with starch, sucrose, amino sugar, nucleotide sugar, ascorbate, aldarate, cellulose, and chitin degradation, thus, increasing organic C mineralization. Among the modified-biochar treatments, higher C mineralization was recorded in SBF, resulting in increased cumulative CO2 emission, despite its initial capture for up to 42 days. However, MgOBF was effective in capturing soil-derived CO2, despite the increased C mineralization compared to biochar. The changes in soil moisture and temperature significantly regulated NCER. Also, the modified biochars positively influenced the distribution of C-cycling-related bacteria by improving soil pH and available nutrients. Among the modified biochars, the observed higher mitigation effect of MgOBF on NCER indicated that it could be preferably applied in agricultural soils.

Biochar-fertilizer interaction modifies N-sorption, enzyme activities and microbial functional abundance regulating nitrogen retention in rhizosphere soil.

The impact of the excessive use of N fertilizer remains an environmental problem of global concern. The effect of biochar on soil N retention is still unclear, and knowledge on how a mixture of biochar and fertilizer (B-F) influence N-sorption, N-cycling enzymes activities, diversity and functional abundance of organisms regulating N-retention in rhizosphere soil is poorly understood. Therefore, biochars derived from bamboo, rice straw, cow and pig manure were characterized, and their interactions with NPK fertilizer were evaluated. Results showed that while the effect of biochar on N retention varied among biochar types, such variations increased after B-F. Unlike NH4+ retention, NO3- retention by biochar in fertilized soil was poor (<8 weeks), but were however increased after longer periods (15 weeks) in B-F due to plant uptake, sorption and stimulation of N-cycling enzymes activities. This stimulation proved that N-fertilizer provided substrates for N-cycling organisms which was confirmed by the dominance of Proteobacteria, Chloroflexi, Actinobacteria, and Gemmatimonadetes which are important in soil N-cycling, despite the reductions in total diversity, class, phyla and genera abundance of bacterial 16SrRNA genes by B-F. This suggested that B-F induced specific organisms involved in N-cycling, which out-competed other organisms not involved in N-cycling. The provision of substrates by N-fertilizer in B-F for bacterial groups involved in N-cycling modified the rhizosphere microbial structure. The abundance of N-cycling organisms was regulated by the persistence among dominant groups, soil pH, total N, and microbial colonization induced by different biochars interacting with fertilizer which led to enhanced N-retention.

Short term effects of biochar with different particle sizes on phosphorous availability and microbial communities.

Despite the increasing interest for biochar as a soil amendment, a knowledge gap remains on different particle size of biochar on soil phosphorous (P) availability and its impacts on microbial community. We hypothesized that biochar particle size and incubation temperature can significantly influence soil P availability and microbial community in subtropical acidic soil. A laboratory incubation study was established to investigate the effects of soil pH, available P and soil microbial responses to biochar addition having varying particle sizes using paddy soil and red soil under different incubation temperatures (15 °C & 25 °C). Biochar produced via pyrolysis of spent mushroom substrate feedstock was sieved into three particle sizes ((≤0.5 mm (fine), 0.5-1.0 mm (medium) and 1.0-2.0 mm (large)). The results exhibited that the fine particle biochar resulted in significantly higher release of P, soil pH, available P and bacterial species richness while simultaneously reducing the activities of phosphatase enzyme in both soils. Apprehending the impact of biochar particle size and incubation temperature, principal coordinate analysis (PCoA) predicted that soil microbial communities with fine particle biochar and high incubation temperature (25 °C) clustered separately. Redundancy analysis depicted that fine particle biochar had a direct association with available P and soil pH while high incubation temperature depicted a strong affinity for microbial communities. Hence, it is suggested that fine particle biochar and high incubation temperature may provide better habitat for microorganisms compared to the other particle sizes which may be due to improved soil pH and available P. However, a long term study of different biochar particles application in subtropical acidic soil needs to be pursued further for a more comprehensive understanding on this issue.

Role of biochar and plant growth promoting rhizobacteria to enhance soil carbon sequestration-a review.

Global climate is undergoing significant changes due to extensive release of greenhouse gases (GHGs) such as CO2 and methane in the atmosphere. These gases are produced and released as a result of anthropogenic activities and fossil fuel burnings which also result in depletion of soil carbon resources. Biochar has various distinctive properties, which contribute to make it an effective, economical, and eco-friendly approach for soil carbon sequestration. The versatility in physicochemical properties of biochar provides an opportunity to optimize its efficacy to obtain desired benefits. A critical review of the literature indicates that biochar and plant growth-promoting microbes have the potential to improve soil organic carbon (SOC). Recent studies have depicted a significant role of the combined application of plant growth-promoting microbes and biochar on SOC dynamics. In future, these areas need to be explored as these have the potential to improve SOC dynamics and it could be a better strategy to sustain natural resources and ultimately mitigation of the climate change.

Evaluating the mechanisms of the impacts of key factors on soil soluble organic nitrogen concentrations in subtropical mountain ecosystems.

Soil soluble organic nitrogen (SON) concentrations in terrestrial ecosystems were influenced differently and substantially by both biotic and abiotic factors. This study aimed to ascertain the mechanisms of the impact of the key factors on the SON concentrations of subtropical mountain ecosystems in southeastern China using an integrative approach, which combined a field plot survey, gray relational analysis and structure equation modeling. The results showed that the soil organic matter, clay content, protease activity and bacterial biomass were the key factors controlling the dynamics of the SON concentrations in subtropical mountain ecosystems. Protease activity, by catalyzing the degradation of complex organic nitrogen to SON, had the highest direct influence on the SON concentrations among all of the impact factors with direct impact effect of 0.44. Organic matter, which serves as a primary source of SON and can increase soil protease activity and bacterial biomass, contributed the most significantly to the SON concentrations in both direct and indirect pathways with total impact effects of 0.87. Clay, by adsorbing SON and affecting organic matter accumulation and protease activity, also had important direct or indirect influences on the SON concentrations with total impact effects of 0.48. The impact of the bacterial biomass on the SON concentrations was likely to be concealed by accompanying nitrogen-degrading enzyme activity with total impact effects of 0.22. Thus, the organic matter, clay content and protease activity exerted greater total impact effects on the SON concentrations compared with the bacterial biomass. Protease activity and organic matter had a greater positive direct impact on the SON concentrations compared with the bacterial biomass and clay content, while organic matter also had greater positive indirect impacts on the SON concentrations than did the clay content. This study's results could help to elucidate the differential mechanism of SON dynamics among various terrestrial ecosystems.

Effects of soil data and simulation unit resolution on quantifying changes of soil organic carbon at regional scale with a biogeochemical process model.

Soil organic carbon (SOC) models were often applied to regions with high heterogeneity, but limited spatially differentiated soil information and simulation unit resolution. This study, carried out in the Tai-Lake region of China, defined the uncertainty derived from application of the DeNitrification-DeComposition (DNDC) biogeochemical model in an area with heterogeneous soil properties and different simulation units. Three different resolution soil attribute databases, a polygonal capture of mapping units at 1:50,000 (P5), a county-based database of 1:50,000 (C5) and county-based database of 1:14,000,000 (C14), were used as inputs for regional DNDC simulation. The P5 and C5 databases were combined with the 1:50,000 digital soil map, which is the most detailed soil database for the Tai-Lake region. The C14 database was combined with 1:14,000,000 digital soil map, which is a coarse database and is often used for modeling at a national or regional scale in China. The soil polygons of P5 database and county boundaries of C5 and C14 databases were used as basic simulation units. Results project that from 1982 to 2000, total SOC change in the top layer (0-30 cm) of the 2.3 M ha of paddy soil in the Tai-Lake region was +1.48 Tg C, -3.99 Tg C and -15.38 Tg C based on P5, C5 and C14 databases, respectively. With the total SOC change as modeled with P5 inputs as the baseline, which is the advantages of using detailed, polygon-based soil dataset, the relative deviation of C5 and C14 were 368% and 1126%, respectively. The comparison illustrates that DNDC simulation is strongly influenced by choice of fundamental geographic resolution as well as input soil attribute detail. The results also indicate that improving the framework of DNDC is essential in creating accurate models of the soil carbon cycle.

[Application of GIS and integrated mathematic models on estimating forest land wood productiveness and solar energy use efficiency].

Based on the meteorological elements observation and mountain soil survey in Fujian Province, this paper approached the application of geographic information system (GIS) and integrated mathematic models on estimating the grid wood productiveness and solar energy use efficiency (SEUE) of regional forest land. The results showed that there was a significant quadratic correlation of annual mean temperature, precipitation and total solar radiation energy(TSRE) with longitude, latitude and altitude, and their multiple correlation coefficients ranged from 0.692 to 0.981. The regional annual mean TSRE, temperature and precipitation could be well estimated by GIS and integrated models of quadratic tendency curve, and linear, quadratic and quartic inverse distance weighted interpolation. These annual means estimated by the models did not differ greatly from observed data, and the t test values were 1.29, 0.12 and 0.06, respectively. The grid wood productiveness and SEUE of regional forest land in Fujian could also be well estimated with the aid of GIS and integrated models, which ranged from 2.32 m3 x hm(-2) yr(-1) to 18.61 m3 x hm(-2) yr(-1) and from 0.11% to 0.91%, respectively.