RESUMO
Objective: To inquiry the changes of serum miRNAs expression in pneumoconiosis complicated with pulmonary tuberculosis patients. Methods: The study subjects (all subjects are male) were divided into three groups: â stage pneumoconiosis (38 cases) ãPneumoconiosis complicated with pulmonary tuberculosis (12 cases) and healthy control (40cases) . The first two groups come from Tianjin worker's sanatorium and healthy control group came from a Hospital Health Screening Center. RT-qPCR was used to detect the expression levels of miR-21, miR-200c, miR-16, miR-204, miR-206, miR-155, let-7g, miR-30b, miR-192, miR-29a in serum. Results: Compared with the healthy control group, the expression levels of miR-204, miR-206, miR-21, miR-16, miR-29a, miR-155, miR-200c in other two groups are different (P<0.05) ; Compared with the â stage pneumoconiosis, the expression levels of miR-155 is significantly higher (P<0.05) and miR-29a is significantly lower (P<0.05) . Conclusion: Compared with the â stage pneumoconiosis, the expression levels of miR-155 and miR-29a are significantly different, suggesting that these two miRNAs may play a important role in the process of pneumoconiosis complicated with pulmonary tuberculosis.
