From organic fertilizer to the soils: What happens to the microplastics? A critical review.

In recent, soil microplastic pollution arising from organic fertilizers has been of a great increasing concern. In response to this concern, this review presents a comprehensive analysis of the occurrence and evolution of microplastics in organic fertilizers, their ingress into the soil, and the subsequent impacts. Organic fertilizers are primarily derived from solid organic waste generated by anthropocentric activities including urban (daily-life, municipal wastes and sludge), agricultural (manure, straw), and industrial (like food industrial waste etc.) processes. In order to produce organic fertilizer, the organic solid wastes are generally treated by aerobic composting or anaerobic digestion. Currently, microplastics have been widely detected in the raw materials and products of organic fertilizer. During the process of converting organic solid waste materials into fertilizer, intense oxidation, hydrolysis, and microbial actions significantly alter the physical, chemical, and surface biofilm properties of the plastics. After the organic fertilizer application, the abundances of microplastics significantly increased in the soil. Additionally, the degradation of these microplastics often promotes the adsorption of organic pollutants and affects their retention time in the soil. These microplastics, covered by biofilms, also significantly alter soil ecology due to the unique properties of the biofilm. Furthermore, the biofilms also play a role in the degradation of microplastics in the soil environment. This review offers a new perspective on the soil environmental processes involving microplastics from organic fertilizer sources and highlights the challenges associated with further research on organic fertilizers and microplastics.

Construction and Application of a Three-Level Linkage System for the Prevention and Treatment of Pressure Sores in Geriatric Patients in the Henan Province, China.

Background: To research effective prevention and treatment strategies for pressure sores in geriatric patients and examine the results from application of a three-level linkage system. Methods: We developed and constructed a three-level linkage intervention system for pressure sores from Jun 2017 to Dec 2018, centered at the geriatrics department of the Ninth People's Hospital of Zhengzhou, China. The changes included improving the organization structure; formulating a unified evaluation system for quantitation of pressure sore risk management; formulating and standardizing the reporting/feedback mechanism; constructing and improving three-level linkage system staff training; and establishing a quality control system for process monitoring guidance and final evaluation feedback. Results: The incidence of pressure sores significantly decreased, nursing staff's knowledge level regarding pressure sore prevention and treatment increased, and pressure sore cure rate and care satisfaction increased. Conclusion: Implementation of a three-level linkage intervention system for pressure sores in geriatric patients and standardizing pressure injury assessment helps achieve pressure sore prevention and early intervention, effectively reduces the occurrence of pressure sores in geriatric nursing homes, increases the cure rate, and improves care satisfaction among patients.

Anaerobic co-digestion of Chinese cabbage waste and cow manure at mesophilic and thermophilic temperatures: Digestion performance, microbial community, and biogas slurry fertility.

The objective of this study was to investigate the effects of mixing ratios of Chinese cabbage waste (CCW) and cow manure (CM) on methane yields, microbial community, and biogas slurry fertility during anaerobic co-digestion. Batch experiments were conducted at mesophilic and thermophilic temperatures with five different CCW/CM mixing ratios. Methane yields at mesophilic and thermophilic temperatures were 4.2-184.4 mL g-1 Volatile solidsadded (mL g-1 VSadded) and 11.8-321.7 mL g-1 VSadded, respectively. The richness and diversity of bacteria and archaea at mesophilic temperatures were higher than those at thermophilic temperatures. Compared with the unfertilized control, the dry weight of corn seedlings with the follow-up application of mesophilic or thermophilic biogas slurry increased by 12.3 %-73.4 % or 16.8 %-43.3 %, respectively. This study demonstrates that thermophilic temperatures are conducive to increasing methane yields, but mesophilic temperatures are conducive to improving the biogas slurry fertility.

lncRNA NORAD promotes the progression of osteosarcoma via targeting of miR-155-5p.

Osteosarcoma (OS) is the most common malignant bone tumor in teens. Non-coding RNA activated by DNA damage (NORAD), a long non-coding RNA (lncRNA), has been reported to be involved in cancer biology, although its role in OS remains largely unknown. In the present study reverse transcription-quantitative PCR (RT-qPCR) was used to determine the expression levels of NORAD and miR-155-5p in samples from patients with OS. OS cell lines (Saos-2 and U2OS) were used as cell models. The biological influence of NORAD on OS cells was studied in vitro using Cell Counting Kit-8 and Transwell assays. The interaction between NORAD and miR-155-5p was clarified by bioinformatics analysis, RT-qPCR, luciferase reporter assay and RNA immunoprecipitation. NORAD was significantly increased in OS samples in comparison with controls, while miR-155-5p was reduced. Knockdown of NORAD and transfection of miR-155-5p mimics markedly inhibited the viability, migration and invasion of OS cells. There was a negative correlation between NORAD and miR-155-5p expression levels in OS samples. Taken together, the results of the present study indicated that the NORAD/miR-155-5p axis played a crucial role in regulating the proliferation, migration and invasion of OS cells. It is hypothesized that NORAD and miR-155-5p may serve as potential novel therapeutic targets for OS management.