RESUMO
Objective To investigate the role of the hypothalamus, medullary visceral zone (MVZ)and vagus in cerebral hemorrhage (CH) in multiple organ dysfunction syndrome (MODS) rat model.To reveal the possible regulatory mechanism of the hypothalamus-MVZ-vagus pathway in CMODS.Methods 80 Wistar rats were randomly divided into sham-operative group (n = 10), subdiaphragmatic vagotomy group (SDV, n = 10), CH group (n = 20), CH + SDV group (n = 20) and CH + vagus stimulation group (CH +SIV, n = 20).Rat CH model was made by stereotaxis injection of collagenase in caudate nucleus.Its endotoxin in serum was evaluated with a test kit.The expression of FOS in the hypothalamus and CHAT in the MVZ in the rats were examined using immunohistochemistry methods.Results Abnormality of hepatic and renal function, myocardium enzyme in CH + SDV group, CH + SIV group and CH group were more severe than those in sham-operative group and SDV group; CH + SDV group more severe than CH group.CH +SIV group were much milder than CH group.The serum endotoxin level (EU/ml) in CH group,compared with the sham-operated group, were significantly different (1.03 ± 0.25 and 0.18 ± 0.03respectively, t = 10.61, P <0.01).The expression of FOS in hypothalamus of CH + SDV group (9.3 ±2.6) were significantly higher than those in sham-operative group (2.5 ± 0.4, t = 8.17, P < 0.01) and SDV group (2.9 ±0.5, t =7.64, P <0.01), but significantly lower than those in CH group (48.6 ± 18.1,t =6.80, P<0.01); was higher in CH +SIV group than those in CH group (74.3 ±6.3 and 48.6 ± 18.1,t=4.23, P <0.01).The expression of CHAT in MVZ in CH + SDV group (138.5 ± 11.8) was significantly higher than those in sham-operative group (108.2 ±9.5, t =5.39, P <0.01) and SDV group (110.7 ±9.9, t =4.83, P <0.01), but significantly lower than those in CH group (164.4 ± 12.4, t =3.53, P <0.01); was higher in CH + SIV group than in CH group (189.4 ± 15.0 and 164.4 ± 12.4, t =3.14, P < 0.05).Conclusion Hypothalamus may be related to the nerve immunity adjustment in MODS after cerebral hemorrhage.The hypothalamus-MVZ-vagus pathway may be distinctive in CMODS.Vagus nerve may be a Bi-directional Channel of the rats following cerebral hemorrhage and MODS.
RESUMO
Objective To explore the effects of the vagus and its transmitter on cerebrogenic multiple organ dysfunction syndrome induced by cerebral hemorrhage(CH) in rats. Methods 80 wistar rats were randomly divided into sham-operative group (n=10),subdiaphringmatic vagotomy (SDV) group (n=10),CH group (n=20), CH+SDV group (n=20) and CH+SIV group (n=20).Rat CH model was made by stereotaxis injection of collagenase and heparin sodium in caudate nucleus. The success full CMODS rat model was characterized by the abnormality of hepatic and renal function,myocardium enzyme, blood routine and vital sign of rat chalked standard. The rats of CH+SDV group were practiced SDV operation 4 weeks before the model was made. CH+SIV group were put electrostimulation of vagus after the mode was made for 6 times. The stimulus continued 20 min with 4 h interval. All rats were executed 24 h after CH. The pathological change of main organs (liver, kidney, lung, small intestine),blood routine were tested. Myocardium enzyme,hepatic and renal function were examinied. Meanwhile, the expression of FOS,CHAT and VIP in the medullary visceral zone of the rats were examined with immunohistochemistry,compared with the sham-operative group and SOV gruop. Results Abnormality of hepatic and renal function,myocardium enzyme and the pathological change of liver,kidney,small intestine CH+SDV group,CH+SIV group and those of CH group outweigh than sham-operative group and SDV group(all P
