Aberrantly methylated-differentially expressed genes and related pathways in cholangiocarcinoma.

ABSTRACT: This study aimed to explore aberrantly methylated-differentially expressed genes and related pathways in cholangiocarcinoma (CCA).The mRNA expression data (GSE26566) and methylation profiling data (GSE44965) were collected from the Gene Expression Omnibus (GEO) Datasets. Differentially expressed genes and differentially methylated genes were identified using GEO2R. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using clusterprofiler in R. MCODE clustering tool was used to screen modules of the protein-protein interaction network in Cytoscape. Related pathways of hub gene by using gene set enrichment analysis.Eighty-one hypermethylated, lowly expressed genes (Hyper-LGs) and 76 hypomethylated, highly expressed genes (Hypo-HGs) were identified in this study. Hyper-LGs were enriched in ion channel binding and transcription factor activity, which was associated with Mineral absorption and Cell adhesion molecules. Hypo-HGs were enriched in cysteine-type endopeptidase activity, which was associated with Sphingolipid signaling pathway and T cell receptor signaling pathway. Based on protein-protein interaction networks, MYC and VWF were identified as hub genes for Hyper-LGs, and no hub genes for Hypo-HGs.This study found methylated-differentially expressed genes and signaling pathways that are connected with the CCA by using a series of bioinformatics databases and tools. MYC and VWF act as hub genes of CCA, which can be used as biomarkers based on aberrant methylation for the accurate diagnosis and treatment of CCA.

Epidemiology and lifestyle survey of non-alcoholic fatty liver disease in school-age children and adolescents in Shenyang, Liaoning.

BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is diagnosed increasingly in children and adolescents. We aimed to investigate the prevalence and related influencing factors of NAFLD in school-aged children and adolescents in Shenyang, Liaoning Province. Furthermore, we analyzed the relationship between lifestyle and fatty liver. METHODS: We conducted aprospective cohort study of 1309 school-aged children and adolescents between the ages of 7 and 18 years who underwent physical examination from November to December 2019. In addition, they were collected age, gender, learning stage, height, weight, BMI, waist circumference, hip circumference, and waist-hip ratio. Finally, a portion of the population was selected to complete a questionnaire survey to explore the impact of lifestyle habits on fatty liver disease. RESULTS: NAFLD was present in 23.83% of subjects. The prevalence of children and adolescents was 22.73% and 24.43%, respectively. Fatty liver prevalence differs significantly by gender and learning stages. The highest rate of fatty liver was seen in obese children (71.68%). Moreover, exercise, diet, and parental factors can affect children with fatty liver. CONCLUSIONS: NAFLD is very prevalent in children and adolescents in Shenyang city. Due to the close relationship between NAFLD and obesity, lifestyle plays a major role in the occurrence of NAFLD. TRIAL REGISTRATION: The First Affiliated Hospital of China Medical University, [2020] 2020-258-2. Registered 6 June 2020-Retrospectively registered.

Comparative study on the repairing effect of stamp skin and meek skin on wounds in patients with extensive burns / 中国医师杂志

Objective:To explore the difference in the therapeutic effect of stamp skin and meek skin on wound repair in patients with extensive burns.Methods:A total of 81 patients with extensive burn from March 2016 to February 2018 in 73th Army Hospital of PLA were selected and divided into group A (stamp skin grafting, 35 cases) and group B (meek skin grafting, 46 cases) according to the choice of wound repair methods before operation. The survival and healing conditions, treatment costs, mortality and rehabilitation of the two groups were compared.Results:There was no significant difference in the survival rate, wound healing rate and mortality between group A and group B (82.86% vs 86.96%, 5.71% vs 8.70%, P>0.05). The survival rate of skin graft in group A was higher than that in group B, and the wound healing time and treatment cost of 1% total body surface area (TBSA) in group A were lower than those in group B [(76.3±5.1)% vs (67.9±6.2)%, (41.5±4.9)d vs (45.8±5.1)d, (1 215.6±235.1)yuan vs (7 689.5±681.0)yuan, P<0.05]. The excellent and good rate of rehabilitation in group A was significantly lower than that in group B (68.57% vs 86.96%, P<0.05). Conclusions:The application of the stamp skin in the repair of wounds in large-area burn patients has a higher flap survival rate than meek skin repair, which can shorten the healing time of the flap and reduce the treatment cost, but the rehabilitation effect is poor.

Experimental study on the damage to cells from fat removal and suction under negative pressures / 中华医学美学美容杂志

Objective To study the injury percentage of fat cells from human bodies by three methods and four suctioned negative pressures. Methods Fat tissue was removed from lower abdomen by excision, aspiration and suction. A pair of sharp scissors was used to cut excisied adipose tissue into 3mm diameter fat particles. A 4 mm diameter pipet and four negative pressures (-25 kPa, -50 kPa, -76 kPa and -101 kPa) were used to suction fat tissue. The samples were fixed in 10 % buffered formalin, stained with hematoxylin eosin and Wilder's silver methods. The fat cells were observed under light microscope. Results The injury percentage of fat cells removed by three methods were (8.6? 1.8) %, (15.7?2.4) % and (38.4?4.2) % respectively;all of them had significant differences among them. The injury percents of fat cells suctioned under-25 kPa,-50 kPa, -76 kPa and -101 kPa were (26.3?2.5) %, (27.6? 2.6) %, (36.8?3.5) % and (68.9?7.4) % respectively. There were no significant differences between the first two but there were among other groups. Conclusions The injury extent of fat cells has significant differences among 3 methods, in which the excision is the least and the suction is the most serious. The injury extent of fat cells has no significant differences when the pressure is lower than -25 kPa. But when the pressure is higher than -50 kPa, the higher the pressure, the more serious the injury extent.