RESUMO
A rat model of ventilation-induced lung injury (VILI) during anesthesia was generated to investigate the potential role and possible mechanism of interleukin-10 (IL-10) and recombinant human keratinocyte growth factor-2 (rhKGF-2) in protecting anesthetized rats against VILI. A total of 50 male SD rats were randomly divided into 5 groups (N = 10 each): control, VILI, IL-10, rhKGF-2, and IL-10 + rhKGF-2. The VILI (model) group was generated via ventilation, with a tidal volume of 20 mL/kg. Rats in the IL-10 and rhKGF-2 groups received 8 mg/kg IL-10 and 5 mg/kg rhKGF-2, respectively, prior to ventilation. The rats in the IL-10 + rhKGF-2 group received both 8 mg/kg IL-10 and 5 mg/kg rhKGF-2 72 h before ventilation. The total number of nucleated cells and neutrophils in the bronchial alveolar lavage fluid was quantified, and the pathological changes in the pulmonary tissues examined by hematoxylin and eosin staining. The transcript and protein levels of surfactant protein C (SP-C) in lung tissues were detected by real-time polymerase chain reaction and western blot analyses. The SP-C mRNA expression in both IL-10 and rhKGF-2 groups was similar to that in the VILI group. However, this was significantly elevated in the combined treatment group (P < 0.05), indicating that IL-10 and rhKGF-2 could synergistically protect the lung tissue from VILI via the enhancement of SP-C mRNA expression in lung tissues. The protein assay showed a decreased level of infiltration and activation of inflammatory cells, in addition to increased expression of SP-C, thereby confirming the efficacy of this treatment in preventing VILI during anesthesia.
Assuntos
Fator 10 de Crescimento de Fibroblastos/farmacologia , Interleucina-10/farmacologia , Substâncias Protetoras/farmacologia , Proteínas Recombinantes/farmacologia , Lesão Pulmonar Induzida por Ventilação Mecânica/patologia , Animais , Biomarcadores , Líquido da Lavagem Broncoalveolar , Contagem de Células , Modelos Animais de Doenças , Humanos , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Pulmão/patologia , Masculino , Infiltração de Neutrófilos , Proteína C Associada a Surfactante Pulmonar/metabolismo , RNA Mensageiro/genética , Ratos , Lesão Pulmonar Induzida por Ventilação Mecânica/tratamento farmacológico , Lesão Pulmonar Induzida por Ventilação Mecânica/genética , Lesão Pulmonar Induzida por Ventilação Mecânica/metabolismoRESUMO
BACKGROUND: Acute aortic dissection (AAD) is a life-threatening emergency. Patients with AAD who have abdominal pain are easily confused with patients with surgical acute abdomen. Matrix metalloproteinases (MMPs) play an important role in the pathophysiology of AAD. This study was undertaken to compare serum MMP-9 levels in patients with acute abdomen and those with AAD presenting as abdominal pain in the emergency department. METHODS: Blood samples were collected within 1 h and 24 h after admission to the emergency department. Serum levels of MMP-9, lipoprotein (a) (LP(a)) and high-sensitivity C-reactive protein (hsCRP) were measured in 20 healthy controls, 20 patients with acute pancreatitis, 20 with other acute abdomen and 20 patients with AAD with abdominal pain by enzyme-linked immunosorbent assay and immunoturbidimetric assay, respectively. RESULTS: Serum MMP-9, LP(a) and hsCRP levels were significantly higher in the three groups of patients than in the healthy controls, with no significant fluctuation within 24 h of admission in any group. Mean serum MMP-9 levels in patients with acute pancreatitis (768 (95% CI 651 to 885) ng/ml within 1 h; 708 (95% CI 677 to 740) ng/ml at 24 h) were significantly higher than in patients with other acute abdomen (244 (95% CI 182 to 266) ng/ml within 1 h; 259 (95% CI 219 to 299) ng/ml at 24 h) and lower than in patients with AAD (1052 (95% CI 921 to 1183) ng/ml at 1 h; 1107 (95% CI 973 to 1241) ng/ml at 24 h) (all p<0.05). No significant difference was detected in serum LP(a) and hsCRP levels among the three groups of patients. CONCLUSIONS: Patients with AAD who have abdominal pain have significantly higher serum MMP-9 levels than patients with surgical acute abdomen.
Assuntos
Dor Abdominal/sangue , Aneurisma da Aorta Abdominal/sangue , Metaloproteinase 9 da Matriz/sangue , Pancreatite/sangue , Abdome Agudo/sangue , Dor Abdominal/enzimologia , Doença Aguda , Adulto , Idoso , Idoso de 80 Anos ou mais , Aneurisma da Aorta Abdominal/diagnóstico , Aneurisma da Aorta Abdominal/enzimologia , Biomarcadores/sangue , Proteína C-Reativa/análise , Diagnóstico Diferencial , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Lipoproteína(a)/sangue , Masculino , Pessoa de Meia-Idade , Pancreatite/diagnóstico , Pancreatite/enzimologiaRESUMO
To analyze brain abscess for its origins of infection, diagnosis, and methods of treatment, 48 patients with brain abscess were retrospectively reviewed and they were treated with nonoperative way, puncture and aspiration, and resection. The results were that the cure rate was 81.25%, the improvement was 12.5%, and the mortality was 6.25%. The chronic otogenic infection and cryptogenic infection are the main origins of infection of brain abscess. The diagnosis of brain abscess depends on CT and MRI, and methods of its treatment are decided by the condition and speciality of brain abscess.
