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Sci Signal ; 6(302): ra100, 2013 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-24255177

RESUMO

Agrobacterium-mediated transformation is the most widely used technique for generating transgenic plants. However, many crops remain recalcitrant. We found that an Arabidopsis myb family transcription factor (MTF1) inhibited plant transformation susceptibility. Mutating MTF1 increased attachment of several Agrobacterium strains to roots and increased both stable and transient transformation in both susceptible and transformation-resistant Arabidopsis ecotypes. Cytokinins from Agrobacterium tumefaciens decreased the expression of MTF1 through activation of the cytokinin response regulator ARR3. Mutating AHK3 and AHK4, genes that encode cytokinin-responsive kinases, increased the expression of MTF1 and impaired plant transformation. Mutant mtf1 plants also had increased expression of AT14A, which encodes a putative transmembrane receptor for cell adhesion molecules. Plants overexpressing AT14A exhibited increased susceptibility to transformation, whereas at14a mutant plants exhibited decreased attachment of bacteria to roots and decreased transformation, suggesting that AT14A may serve as an anchor point for Agrobacteria. Thus, by promoting bacterial attachment and transformation of resistant plants and increasing such processes in susceptible plants, treating roots with cytokinins may help engineer crops with improved features or yield.


Assuntos
Agrobacterium tumefaciens/genética , Proteínas de Arabidopsis/genética , Arabidopsis/genética , Citocininas/metabolismo , Fatores de Transcrição/genética , Agrobacterium tumefaciens/metabolismo , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Citocininas/fisiologia , Regulação da Expressão Gênica de Plantas , Histidina Quinase , Mutação , Análise de Sequência com Séries de Oligonucleotídeos , Plantas Geneticamente Modificadas , Proteínas Quinases/genética , Proteínas Quinases/metabolismo , Interferência de RNA , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transdução de Sinais/fisiologia , Fatores de Transcrição/metabolismo , Transcriptoma
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