1.
J Colloid Interface Sci
; 405: 291-5, 2013 Sep 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23746437
RESUMO
Amino-functionalized Fe3O4 magnetic nanoparticles with high peroxidase-like activity (MNPs-NH2) are reported in this work. The peroxidase-like activity is approximately the same as naked Fe3O4 magnetic nanoparticles (MNPs). MNPs-NH2 displayed much better thermal stability and pH tolerance than horseradish peroxidase (HRP). The optimal pH and temperature are approximately pH 4 and 40 °C, respectively. We showed that the MNPs-NH2 were successfully applied in a double-antibody sandwich "enzyme"-linked immunosorbent assay to replace of HRP, in which MNPs-NH2 were not only separation carriers, but also detection indicator.