Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly.

Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequences at virtually any arbitrary site. The present review focuses on the commonly used in vivo and in vitro recombination-based gene cloning methods and the application of programmable endonucleases in these sequence- and ligation-independent DNA assembly methods. The advantages and shortcomings of the programmable endonucleases utilized as tools for gene cloning are also discussed in this review.

Serum Gc-globulin levels are reduced in patients with severe acute pancreatitis.

BACKGROUND AND AIM: To investigate the role of group-specific component globulin (Gc-globulin) in the early process of severe acute pancreatitis (SAP). PATIENTS AND METHODS: A total of 37 patients with SAP and 31 patients with mild acute pancreatitis (MAP) were enrolled in the study. Twenty healthy individuals served as controls. Blood samples were taken from SAP and MAP patients on the day of admission (day 1), day 7, and day 14. Serum total Gc-globulin levels were measured using an enzyme-linked immunosorbent assay. RESULTS: On admission, the total Gc-globulin levels of patients in the SAP group were 368 (270-455) mg/l (expressed as medians with interquartile range), which were significantly lower (P=0.007) compared with the control group [420 (299-585) mg/l]. In the SAP group, a significant decrease in serum total Gc-globulin was observed in patients who developed organ dysfunction [267 (242-306) mg/l] compared with patients who did not [414 (329-513) mg/l, P<0.001]. Patients with lower Gc-globulin levels were at a higher risk of developing organ dysfunction (P=0.005). Furthermore, serial measurements displayed increasing total Gc-globulin levels in survivors. CONCLUSION: Decreased total Gc-globulin was linked to poor outcomes of SAP. These data support our hypothesis that Gc-globulin may play an important role in the early process of acute pancreatitis. Gc-globulin might be a novel target for prognosis and therapy of SAP.