RESUMO
OBJECTIVE: To compare the effect of various fluid resuscitation procedures after hemorrhagic shock on the lactic acid accumulation by network meta-analysis. MATERIALS AND METHODS: A number of articles were obtained by searching the databases. Randomized control trials (RCT) were selected by two literature quality assessors; the extracted experimental data were then summarized to compare and analyze the effect of various kinds of infusion procedures at different time points on the concentration of lactic acid in the internal environment of rat models. RESULTS: 9 articles containing 25 independent reports were enrolled. Results revealed that the accumulation of lactic acid generated in the resuscitation using hydroxyethyl starch solution or gelofusine was significantly different from the one using Ringer solution, hypertonic saline or normal salt solution (p<0.05). Compared to the colloidal, the aqueous solution of pyruvate showed a slightly weaker effect in reducing the accumulation of lactic acid. The accumulation of lactic acid is severely affected by hypertonic saline, Ringer lactate solution, and balanced salt solution, i.e., the concentration of lactic acid is increasing with an augment of concentration or prolongation of treatment time. CONCLUSIONS: In terms of the management of lactic acid accumulation after hemorrhagic shock, the effect of colloidal solution was remarkably better than a crystalloid solution, and hypertonic saline and normal salt solution were not recommended as preferable drugs in the treatment of hemorrhagic shock.
Assuntos
Hidratação/métodos , Ácido Láctico/sangue , Substitutos do Plasma/administração & dosagem , Ressuscitação/métodos , Choque Hemorrágico/terapia , Coloides , Soluções Cristaloides/administração & dosagem , Humanos , Metanálise em Rede , Prognóstico , Ensaios Clínicos Controlados Aleatórios como Assunto , Índice de Gravidade de Doença , Choque Hemorrágico/sangue , Choque Hemorrágico/diagnóstico , Resultado do TratamentoRESUMO
OBJECTIVE: Non-small cell lung cancer (NSCLC), as an ordinary malignant tumor, presents with high death rate and poor prognosis. Few literatures have explored the association between NSCLC development and lncRNAs expression. This study focuses on the important role of a novel lncRNA TRPM2-AS in the development of chemo-resistance in NSCLC. MATERIALS AND METHODS: The expression level of lncRNA TRPM2-AS was identified by using qRT-PCR assay. The apoptosis rate and the alteration of the cell cycle were detected by the flow cytometric analysis. Cell Counting Kit-8 assay (CCK8) was utilized for detecting chemo-sensitivity of the cisplatin-resistant A549/DDP cells. The p53 and p66shc protein levels were detected by Western blotting assay. RESULTS: A549/DDP cells presented remarkably higher expression of lncRNA TRPM2-AS than paired A549 cells. Moreover, re-sensitization to cisplatin was seen in A549/DDP cells after lncRNA TRPM2-AS knockdown. On the contrary, the sensitivity of lncRNA TRPM2-AS-overexpressed A549 cells to cisplatin decreased obviously when compared with the control. Furthermore, downregulated lncRNA TRPM2-AS induced cell apoptosis and altered cell cycle distribution through activating the p53-p66shc pathway. CONCLUSIONS: We suggest that lncRNA TRPM2-AS participates in the resistance of NSCLC cells to cisplatin, which may provide a new therapeutic target of NSCLC.
Assuntos
Antineoplásicos/farmacologia , Carcinoma Pulmonar de Células não Pequenas/genética , Cisplatino/farmacologia , Resistencia a Medicamentos Antineoplásicos/genética , Neoplasias Pulmonares/genética , RNA Longo não Codificante/genética , Proteína 1 de Transformação que Contém Domínio 2 de Homologia de Src/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Células A549 , Apoptose/efeitos dos fármacos , Carcinoma Pulmonar de Células não Pequenas/patologia , Ciclo Celular/efeitos dos fármacos , Regulação para Baixo , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Humanos , Neoplasias Pulmonares/patologia , Transdução de SinaisRESUMO
Horizontal transfer (HT), the exchange of genetic material between species, plays important roles in transposon biology and genome evolution. In this study, we provide the first documented example of a new Academ transposon involved in recent and distant HTs into the genomes of species belonging to seven different orders of insects: Lepidoptera, Hymenoptera, Neuroptera, Embioptera, Dermaptera, Trichoptera and Zoraptera. These results suggest that HT of DNA transposons amongst insects has occurred on a broader scale than previously appreciated. The Academ transposon discovered in the Lepidoptera and parasitic wasps is of particular interest because the intimate association between wasps and their lepidopteran hosts might provide an opportunity for HT of transposons.
