RESUMO
The protozoans include many intracellular human pathogens. Accurate detection of these pathogens is necessary to treat the diseases. In clinical epidemiology, molecular identification of protozoan is considered a more reliable and rapid method for identification than microscopy. Among these protozoans, Cryptosporidium considered being one of the important water-borne zoonotic pathogens and a major cause of a diarrheal disease named cryptosporidiosis in humans, domestic animals, and wild animals. This study was aimed to identify Cryptosporidium in zoo felids (N= 56) belonging to different zoo of China, but accidentlly Colpodella was encountered in the zoo felids sample and phylogenetic data confirmed this unexpected amplification from fecal samples using two-step nested-PCR. Phylogenetic analysis revealed the fact about the specific primers used previously by many researchers and cross-genera amplification. We came to know that genetically sequenced amplicon gives more accurate identification of species. This study suggests more investigation on Colpodella which has been neglected previously but gains the attention of researchers after identified from humans and animals and has been known to correlate with neurological symptoms in patients.
Os protozoários incluem muitos patógenos humanos intracelulares. A detecção acurada desses patógenos é necessária para tratar as doenças. Na epidemiologia clínica, a identificação molecular de protozoários é considerada o método de identificação mais confiável e rápido do que a microscopia. Entre esses protozoários, o Cryptosporidium é considerado um dos importantes patógenos zoonóticos transmitidos pela água e uma das principais causas de uma doença diarreica denominada criptosporidiose em humanos, animais domésticos e selvagens. Este estudo teve como objetivo identificar Cryptosporidium em zoofelídeos (N = 56) pertencentes a diferentes zoológicos da China, mas acidentalmente Colpodella foi encontrada na amostra de zoofelídeos e os dados filogenéticos confirmaram essa amplificação inesperada de amostras fecais usando nested-PCR em duas etapas. A análise filogenética revelou o fato sobre os primers específicos usados anteriormente por muitos pesquisadores e a amplificação entre gêneros. Ficamos sabendo que o amplicon sequenciado geneticamente fornece uma identificação mais acurada das espécies. Este estudo sugere mais investigação sobre Colpodella, que foi negligenciada anteriormente, mas ganha a atenção dos pesquisadores depois de identificada em humanos e animais e é conhecida por se correlacionar com sintomas neurológicos em pacientes.
Assuntos
Animais , Animais de Zoológico , Cryptosporidium/genética , Cryptosporidium/patogenicidade , Reação em Cadeia da PolimeraseRESUMO
AIM: The aim of this paper was to investigate the application value of the intensive care nursing scoring system (ICNSS) in intensive care unit (ICU) nursing resources allocation. METHODS: The English version of ICNSS scale was translated into Chinese by nursing experts, and was consistently revised and improved based on China's actual conditions. A total of 105 cases were randomly divided into the observation group (N.=55) and the control group (N.=50). In the observation group, the nursing workload was estimated and the nurses were allocated using ICNSS; in the control group, the sickbeds were equally distributed to the nurses and each nurse was equally in charge of managing patients. The ICU duration, medical charge, incidence of complications, nursing quality control scoring and nurses' satisfaction to their resource allocations were compared between the two groups. RESULTS: The ICU duration, medical charge and incidence of complications in the observation group were significantly lower than those in the control group (P=0.032, P=0.024, P=0.006 respectively); while quality control scoring and nurses' satisfaction to their resources allocation in the observation group were significantly higher than those in the control group (P=0.045, P=0.004 respectively). CONCLUSION: The nursing quality and the nurses' satisfaction could be improved using ICNSS to evaluate ICU nursing workload and allocate resources.
