A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification.

There are many PCR-based methods for animal species identification; however, their detection numbers are limited or could not identify unknown species. We set out to solve this problem by developing a universal primer PCR assay for simultaneous identification of eight animal species, including goat, sheep, deer, buffalo, cattle, yak, pig, and camel. In this assay, the variable lengths of mitochondrial DNA were amplified using a pair of universal primers. PCR amplifications yielded 760 bp, 737 bp, 537 bp, 486 bp, 481 bp, 464 bp, 429 bp, and 359 bp length fragments for goat, sheep, deer, buffalo, cattle, yak, pig, and camel, respectively. This primer pair had no cross-reaction with other common domestic animals and fish. The limit of detection varied from 0.01 to 0.05 ng of genomic DNA for eight animal species in a 20 µl PCR mixture. Each PCR product could be further digested into fragments with variable sizes and qualitative analysis by SspI restriction enzyme. This developed PCR-RFLP assay was sufficient to distinguish all targeted species. Compared with the previous published related methods, this approach is simple, with high throughput, fast processing rates, and more cost-effective for routine identification of meat in foodstuffs.

Rounded Cu2ZnSnS4 nanosheet networks as a cost-effective counter electrode for high-efficiency dye-sensitized solar cells.

Semi-transparent rounded Cu2ZnSnS4 (CZTS) nanosheet networks were in situ grown on a FTO glass substrate, via an effective solution method, without any post-treatments. An improved power conversion efficiency of 6.24% was obtained by applying CZTS nanosheet networks as a counter electrode for dye-sensitized solar cells. When assisted by a mirror reflection, the PCE increased to 7.12%.

[Food habits and hunting patterns of Tibetan brown bear during warm seasons in Kekexili region on Qinghai-Tibetan Plateau].

Tibetan brown bear (Ursus arctos pruinosus) is an endemic subspecies of brown bear on the Qinghai-Tibetan plateau. We once reported a preliminary study on the summer food habit of Tibetan brown bear from July to August, 2005 in the Kekexili (Hoh Xil).. However, the hunting modes of the Tibetan brawn bear have not been reported. From July to August, 2009, we collected additional data on food habits and hunting patterns of Tibetan brown bear in the Kekexili region. We found Tibetan brown bears are more raptatorial than vegetarian, and their basal food was plateau pika (Ochotona curzoniae), relative frequency occurrence of pika in the fecal residual of brawn bear was 37.3%, dry weight of pika residuals was 44.7%, respectively, followed by wild yak (Bos grunniens) , 18.7% and 30.2%, and Tibetan antelope (Pantholops hodgsoni) 15.0% and 16.2%. Both prey actively mode and scavenge mode were used by Tibetan brown bears in Kekexili. Tibetan brawn bears actively dug and hunted for pika and scavenged bodies of wild yak, Tibetan antelope and Tibetan gazelle. We observed that Tibetan brawn bears spent about 10% of their time to dig and to hunt for pika but we had never seen Tibetan brawn bears actively hunted large mammals such as wild yak, Tibetan antelope and Tibetan gazelle. The total amount of Tibetan antelope, Tibetan gazelle and wild yak ingested by Tibetan brown bear through scavenge was about the same amount of Plateau pika and Himalayan marmot (Marmota himalayana) eaten by the bear.