RESUMO
<p><b>OBJECTIVE</b>To investigate the effect and mechanism of Shenqi Fuzheng Injection (SFI) in repairing immune function of organism at cellular and molecular levels.</p><p><b>METHODS</b>Seventy-seven patients with pathologically confirmed diagnosis of breast cancer were assigned to three groups, the 22 patients in group A received surgical operation only; the 26 in group B were treated with surgical operation and chemotherapy; and to the 29 in group C, both chemotherapy and SFI were given after surgical operation. Besides, a group D with 22 patients with benign tumor was set up for control. The protein expressions of CD83, CD80 and CD86 in all patients' tumor tissue and axillary lymph node were detected by flow cytometry before and after treatment.</p><p><b>RESULTS</b>Levels of CD83, CD80 and CD86 in both tumor tissue and lymph node of cancer patients were significantly lower than those in the control (P < 0.05); and those in group B were significantly lower than in group A (P < 0.05, P < 0.01); but no significant difference was found in comparing the corresponding indices between group A and group C (P > 0.05).</p><p><b>CONCLUSION</b>The immune function of tumor patients could be impaired by tumor and chemotherapy definitely, and SFI could give aid to the repairing of immunity by way of participating in the activation of dendritic cells and the up regulation of co-stimulus molecules.</p>
Assuntos
Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Antígenos CD , Metabolismo , Antígeno B7-1 , Metabolismo , Antígeno B7-2 , Metabolismo , Neoplasias da Mama , Tratamento Farmacológico , Alergia e Imunologia , Cirurgia Geral , Carcinoma Intraductal não Infiltrante , Tratamento Farmacológico , Alergia e Imunologia , Cirurgia Geral , Medicamentos de Ervas Chinesas , Usos Terapêuticos , Imunidade , Imunoglobulinas , Metabolismo , Glicoproteínas de Membrana , Metabolismo , FitoterapiaRESUMO
AIM: To study the effect of gum mastic, a natural resin, on the proliferation of androgen-independent prostate cancer PC-3 cells, and further investigate the mechanisms involved in this regulatory system, taking nuclear factor kappaB (NF-kappaB) signal as the target. METHODS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and a flow cytometer were used to detect the effect of gum mastic on the proliferation of PC-3 cells. Then, reporter gene assay, RT-PCR, and Western blotting were carried out to study the effects of gum mastic on the NF-kappaB protein level and the NF-kappaB signal pathway. The expression of genes involved in the NF-kappaB signal pathway, including cyclin D1, inhibitors of kappaBs (I kappaB alpha), and phosphorylated Akt (p-AKT), were measured. In addition, transient transfection assays with the 5X NF-kappaB consensus sequence promoter was also used to test the effects of gum mastic. RESULTS: Gum mastic inhibited PC-3 cell growth and blocked the PC-3 cell cycle in the G1 phase. Gum mastic also suppressed NF-kappaB activity in the PC-3 cells. The expression of cyclin D1, a crucial cell cycle regulator and an NF-kappaB downstream target gene, was reduced as well. Moreover, gum mastic decreased the p-AKT protein level and increased the I kappa B alpha protein level. CONCLUSION: Gum mastic inhibited the proliferation and blocked the cell cycle progression in PC-3 cells by suppressing NF-kappaB activity and the NF-kappaB signal pathway.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , NF-kappa B/efeitos dos fármacos , Neoplasias da Próstata/tratamento farmacológico , Resinas Vegetais/farmacologia , Androgênios/fisiologia , Núcleo Celular/química , Núcleo Celular/metabolismo , Proliferação de Células/efeitos dos fármacos , Ciclina D1/biossíntese , Ciclina D1/genética , Humanos , Masculino , Resina Mástique , Proteína Oncogênica v-akt/biossíntese , Proteína Oncogênica v-akt/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transdução de Sinais/efeitos dos fármacosRESUMO
<p><b>OBJECTIVE</b>To search for a better therapy for temporomandibular joint dysfunction syndrome.</p><p><b>METHODS</b>Two hundred and ten cases of temporomandibular joint dysfunction syndrome were randomly divided into a treatment group, a control group I and a control group II, 70 cases in each group. The treatment group were treated with warming needle moxibustion plus exercise, with Hegu (LI 4), Xiaguan (ST 7), Yifeng (TE 17) and so on selected, in combination with opening and closing the mouth; the control group I were treated with simple filiform needle needling, and the control group II with local blocking therapy. The therapeutic effects of the 3 groups were compared.</p><p><b>RESULTS</b>The total effective rate was 94.3% in the treatment group, 87.1% in the control group I and 85.7% in the control group II with a significant difference among the 3 groups, the treatment group being better than the two control groups (P < 0.05).</p><p><b>CONCLUSION</b>Warming needle moxibustion plus exercise has a good therapeutic effect on temporomandibular joint dysfunction syndrome.</p>
