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1.
Materials (Basel) ; 17(10)2024 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-38793269

RESUMO

316L stainless steel pipes are widely used in the storage and transportation of low-temperature media due to their excellent low-temperature mechanical properties and corrosion resistance. However, due to their low thermal conductivity and large coefficient of linear expansion, they often lead to significant welding residual tensile stress and thermal cracks in the weld seam. This also poses many challenges for their secure and reliable applications. In order to effectively control the crack defects caused by stress concentration near the heat-affected zone of the weld, this paper establishes a thermal elastoplastic three-dimensional finite element (FE) model, constructs a welding heat source, and simulates and studies the influence of process parameters on the residual stress around the pipeline circumference and axial direction in the heat-affected zone. Comparison and verification were conducted using simulation and experimental methods, respectively, proving the rationality of the finite element model establishment. The axial and circumferential residual stress distribution obtained by the simulation method did not have an average deviation of more than 30 MPa from the numerical values obtained by the experimental method. This study also considers the effects of welding energy, welding speed, and welding start position on the pipe's circumferential and axial residual stress laws. The results indicate that changes in welding energy and welding speed have almost no effect on the longitudinal residual stress but have a more significant effect on the transverse residual stress. The maximum transverse residual stress is reached at a welding energy of 1007.4~859.3 J/mm and a welding speed of 6.6 mm/s. Various interlayer arc-striking deflection angles can impact the cyclic phase angle of the transverse residual stress distribution in the seam center, but they do not alter its cyclic pattern. They do influence the amplitude and distribution of the longitudinal residual stress along the circumference. The residual stress distribution on the surface of the pipe fitting is homogenized and improved at 120°.

2.
Technol Health Care ; 32(4): 2251-2264, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38517814

RESUMO

BACKGROUND: Psychrophilic bacteria can survive in a unique living environment. OBJECTIVE: To explore the mechanism of low temperature adaptation and the physiological function of thermophilic metabolic genes. METHOD: Serratia marcescens strain F13 stored in microbial laboratory was cultured at 5∘C, 10∘C and 25∘C respectively, and the obtained strains were sequenced by high-throughput transcriptome. Serratia marcescens strain CAV1761 was used as the reference strain. The data produced by transcriptome sequencing were statistically analyzed by biostatistics software such as soapnuke, soap and edger. The differentially expressed genes were found based on the gene expression, and analyzed by Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. RESULTS: The results showed that there were 718 differential genes in F13-10 vs F13-5 comparison group, 1614 differential genes in F13-25 vs F13-5 comparison group and 1636 differential genes in F13-25 vs F13-10 comparison group. GO function enrichment analysis showed that the GO term mainly enriched by different genes in the three comparison groups was mostly related to the migration and transport of cellular or subcellular components, cell localization and transmembrane transporter activity, as well as cilia or flagella dependent cell movement. In the enrichment analysis of KEGG pathway, the three comparison groups all enriched the largest number of differential genes in the branch pathway of KEGG metabolism, followed by the branch pathway of environmental information processing. CONCLUSION: In F13-10 vs F13-5, the differential genes were mainly concentrated in 20 pathways such as ATP-binding cassette transport (ABC) transporters, thiamine metabolism and flagella assembly; In F13-25 vs F13-5, the differential genes are mainly concentrated in 20 pathways, such as (ABC) transporters, arginine and proline metabolism, two-component system and so on; In F13-25 vs F13-10, the differential genes are mainly concentrated in 20 pathways such as various types of glycan synthesis, two-component system and arginine metabolism.


Assuntos
Serratia marcescens , Transcriptoma , Serratia marcescens/genética , Temperatura Baixa , Perfilação da Expressão Gênica/métodos
3.
Sensors (Basel) ; 23(7)2023 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-37050789

RESUMO

With the rapid development of the aerospace industry, the quality inspection of complex curved components, such as aero-engine blades, is becoming increasingly strict. In contrast with other NDT methods, ultrasonic testing is easier to automate, while offering higher accuracy and efficiency in thickness measuring. To solve the challenge of the automated NDT inspection of aero-engine blades, in this study, an ultrasonic inspection system with a six degree of freedom (DOF) was proposed for industrial robots. Additionally, a defect detection model and a thickness detection method were proposed for the robotic ultrasonic inspection system, based on the thickness variation of the aero-engine blade. Through the quantitative analysis on engine blades with prefabricated defects and curved test blocks with stepped thicknesses, it can be concluded that our system is able to achieve high accuracy in defect detection and thickness measurement.

4.
Sensors (Basel) ; 22(23)2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36502132

RESUMO

Great progress has been made in the field of ultrasonic processing in recent years, and piezoelectric ceramic transducers have been widely used as drive sources. In this paper, a sandwich piezoelectric ceramic transducer is designed, and the vibration of each part of the transducer is analyzed by elastic mechanics and piezoelectric theory. According to its mechanical and electrical boundary conditions, the vibration model of the piezoelectric transducer was established. Based on the equivalent elastic modulus method for simplifying the pre-stressed bolts into a one-dimensional transducer vibration model, the relationship between the one-dimensional axial response frequency of the transducer and the length of each component was obtained. Based on the half wavelength theory, a transducer with the vibration node in the crystal stack and an inherent frequency of 15 kHz was designed and fabricated. In order to verify the natural frequency and vibration characteristics of the piezoelectric transducer, a laser vibration measurement system was built in this study. The vibration characteristics of the transducer under different parameters such as voltage and frequency were analyzed, and the accuracy of the vibration model was verified. The vibration states of the end surface of the transducer and the radial surface were evaluated at the first-order inherent frequency and second-order inherent frequency. The results show that the equivalent simplified model established in this study can effectively design the inherent frequency of the transducer, and the operation at the first-order inherent frequency meets the one-dimensional assumptions of this study. The transducer operating conditions measured in this study also provide a more detailed reference for ultrasonic processing applications.


Assuntos
Modelos Teóricos , Transdutores , Desenho de Equipamento , Ultrassonografia/métodos , Cerâmica/química
5.
Materials (Basel) ; 15(18)2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36143566

RESUMO

Due to its extreme service conditions, low-temperature pressure piping often needs post-welding stress measurement and control. Aiming at the phenomenon of local stress concentration in welded 316L pipes, this study used ultrasound to regulate the stress in the welded area at different times during and after the multi-layer welding of the pipeline butt joint for different time lengths. Mechanical properties such as tensile strength and hardness were tested for each comparison group, and the microcrystalline phases of the weld and its surrounding microstructure were analyzed. The transverse and longitudinal surface residual stresses of each comparison group were measured. The influence of high-energy ultrasound on the surface temperature field during and after welding was analyzed. The experimental results show that ultrasonic wave regulation can speed up heat exchange and radiation in the weld zone (WZ), refine the grains in the WZ, heat-affected zone (HAZ) and fusion zone (FZ) to some extent and reduce and homogenize residual stress to a certain degree. In the 120 mm area of the weld center, the residual stress measured after the mid-welding regulation was smaller than that of any other comparison group. This regulation result was the best, followed by that of hot regulation and finally that of offline regulation. The tensile strengths obtained by the mid-welding regulation and post-welding hot regulation of this group were the best, increasing by 17.2% and 24.3%, respectively, compared with the untreated groups.

6.
Ultrasonics ; 125: 106788, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35709573

RESUMO

Longitudinal critically refracted (LCR) waves have already been widely applied for residual stress characterization. Such waves are usually generated using mode-conversion at the first critical angle of the incident longitudinal wave, which gives waves that then propagate at a dip-angle, and this places energy close to the surface of the specimen. The dip-angle needs to be minimized to improve both velocity measurement and residual stress characterization sensitivity. This paper reports a novel double-fold coil phased EMAT that can decrease the dip-angle. The performance of this new EMAT was investigated using both a COMSOL model and experiments. Initial model validation was provided through a comparison with experimental data. The EMAT design also enables scanning of samples, and operation in harsh environments where use of a PZT based transducer and couplants can complicate and limit inspection. The use of the EMAT has the potential to give more accurate time of flight (TOF) data and enhances the reliability and accuracy for residual stress measurement.


Assuntos
Transdutores , Reprodutibilidade dos Testes , Ultrassonografia
7.
Materials (Basel) ; 15(3)2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-35160714

RESUMO

Based on the changes of microhardness, tensile strength, and impact resistance caused by the difference of macroscopic morphology and microstructure of welded joints, this paper studied the effect of different ultrasonic power on the properties of welded joints during the welding of homogeneous armor steel. It is experimentally found that the macroscopic morphology of those joints is very different. Compared with conventional welding, ultrasonic welding can increase the weld depth and the width of the heat-affected zone (HAZ) on either side of the weld. However, only the ultrasonic wave at an appropriate power level can increase the weld width. In addition, appropriate ultrasonic power can significantly improve the grain state of the weld. With the increase of ultrasonic power, the grain size in HAZ will decrease. The microhardness of the weld will first increase and then decrease, while the microhardness of the HAZ will increase. This is basically consistent with the changing trend of impact resistance. An ultrasonic wave can also increase the tensile strength of a welded joint up to 802 MPa, 12.4% higher than that in conventional welding. However, a high-power ultrasonic wave will bring down the tensile strength. This study provides guidance for the selection of ultrasonic-assisted regulation power to achieve the different properties of homogeneous armor steel joints.

8.
Sensors (Basel) ; 19(15)2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31370173

RESUMO

With the rapid development of material science, more and more workpieces, especially workpieces with complex curved surfaces, are being made of composite materials. Robotic non-destructive testing (NDT) systems for complex curved surface composite material parts are being used more and more. Despite the emergence of such flexible NDT systems, the detection of semi-enclosed parts is also a challenge for robotic NDT systems. In order to overcome the problem, this paper establishes an NDT solution for semi-enclosed workpieces based on a dual-robot system of synchronous motion, in which an extension arm is installed on one of the robots and presents a trajectory planning method that always ensures the extension arm is parallel to the rotary axis of a semi-enclosed workpiece and that the ultrasonic probes are perpendicular to the workpiece surface. Trajectory analysis experiments and ultrasonic NDT experiments utilizing the optimal water path distance determined by simulation result of multi-Gaussian beam model for two types of semi-enclosed workpieces are performed with the dual-robot NDT system. Experimental results prove that the dual-robot NDT scheme functions well and the planned trajectories are correct. All the hole-shaped artificial defects with diameters ≥3 mm are detected by using 2.25 MHz ultrasonic probes through the transmission testing method. Vivid 3D C-scan image of a small diameter cylindrical workpiece based on the testing result is provided for convenience of observation.

9.
Sensors (Basel) ; 18(12)2018 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-30544775

RESUMO

Crack assessment when making fitness-for-service decisions requires a thorough examination of crack location and size in critical areas. An ultrasonic transducer is used for such assessments, but traditional methods cannot cope with complex rotators, such as wheel hubs. We present a model of robot-assisted crack growth assessment in wheel hubs. We integrate a six-degrees-of-freedom (DOF) industrial robot and a turntable to form a robot-assisted ultrasonic testing (UT) system that does not use traditional UT equipment. Ultrasonic beams are focused at certain depths appropriate for achieving maximum sensitivity. We quantitatively analysed wheel hubs with longitudinal and transverse series of pre-cracks, and concluded that our system autonomously detected cracks.

10.
Ultrasonics ; 68: 150-4, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26953638

RESUMO

Sensitivity is an important parameter to describe the electro-acoustic energy conversion efficiency of ultrasonic transducer. In this paper, the definition of sensitivity and reciprocity of ultrasonic transducer is studied. The frequency response function of a transducer is the spectrum of its sensitivity, which reflects the response sensitivity of the transducer for input signals at different frequencies. Four common methods which are used to measure the disc-vibrator transducer sensitivity are discussed in current investigation. The reciprocity method and the pulse-echo method are based on the reciprocity of the transducer. In the laser vibrometer method measurement, the normal velocity on the transducer radiating surface is directly measured by a laser vibrometer. In the measurement process of the hydrophone method, a calibrated hydrophone is used to measure the transmitted field. The validity of these methods is checked by experimental test. All of the four methods described are sufficiently accurate for transducer sensitivity measurement, while each method has its advantages and limitations. In practical applications, the appropriate method to measure transducer sensitivity should be selected based on actual conditions.

11.
Sensors (Basel) ; 16(2): 260, 2016 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-26907283

RESUMO

Laminate composites which are widely used in the aeronautical industry, are usually subjected to frequency variation of environmental temperature and excessive humidity in the in-service environment. The thermal fatigue and moisture absorption in composites may induce material degradation. There is a demand to investigate the coupling damages mechanism and characterize the degradation evolution of composite laminates for the particular application. In this paper, the degradation evolution in unidirectional carbon/epoxy composite laminates subjected to thermal fatigue and moisture absorption is characterized by Lamb waves. The decrease rate of Lamb wave velocity is used to track the degradation evolution in the specimens. The results show that there are two stages for the progressive degradation of composites under the coupling effect of thermal cyclic loading and moisture diffusion. The present work provides an alternative to monitoring the degradation evolution of in-service aircraft composite Laminates.

12.
Wei Sheng Wu Xue Bao ; 48(4): 421-5, 2008 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-18590224

RESUMO

OBJECTIVE: Acidophilic filamentous acitnomycetes are active in the turnover of organic matter in acid litters and soils, and are a source of antifungal antibiotics and acid-stable enzymes. The aim of this study is to delineate the diversity of neutrotolerant acidophilic streptomycetes in acidic soil environment, and to investigate the resources of species. METHODS: 367 actinomycetes with cultural characteristics of streptomycetes were isolated from 14 acidic soil samples collected in Yunnan Province, China, using the method of DDC (dispersion and differential centrifugation) and a selective medium. The isolates were color grouped on the basis of their properties of aerial mycelium, substrate mycelium and diffusible pigments. 97 representative isolates were picked from the color groups for micromorphological observation and for the test of pH range for growth. Among the neutrotolerant acidophilic streptomycetes, 16 representatives were further selected and were subjected to a molecular systematic study based on almost complete 16S rRNA gene sequences and DNA-DNA relatedness. RESULTS: The isolates were assigned to 12 color groups, and 80% of them were neutrotolerant acidophilic streptomycetes. The 16 representative strains formed eight distinct subclades within the genus of Streptomyces, and probably represented at least eight new genotypic species of Streptomyces. CONCLUSION: The neutrotolerant acidophilic streptomycetes isolated in this study were placed in eight distinct evolutionary groups, indicating the good diversity and novelty of these microorganisms in acidic soils in Yunnan Province.


Assuntos
Biodiversidade , Microbiologia do Solo , Streptomycetaceae/isolamento & purificação , Streptomycetaceae/fisiologia , China , Genótipo , Concentração de Íons de Hidrogênio , Microscopia Eletrônica , Filogenia , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Streptomycetaceae/genética , Streptomycetaceae/ultraestrutura , Temperatura
13.
Int J Syst Evol Microbiol ; 56(Pt 5): 1109-1115, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16627663

RESUMO

The taxonomic status of six neutrotolerant acidophilic streptomycetes isolated from acidic soils in Yunnan Province, China, was established using a polyphasic approach. The morphological and chemotaxonomic characteristics revealed that the isolates belong to the genus Streptomyces. Almost complete 16S rRNA gene sequences of the isolates were determined and aligned with available corresponding sequences of representatives of the family Streptomycetaceae; phylogenetic trees were inferred using four tree-making algorithms. The isolates formed a distinct, albeit heterogeneous, subclade in the Streptomyces 16S rRNA gene tree together with the type strain of Streptomyces yeochonensis, but were readily distinguishable from the latter using DNA-DNA hybridization and phenotypic data. It was evident from the genotypic and phenotypic data that the isolates belonged to four novel Streptomyces species, for which the following names are proposed: Streptomyces guanduensis sp. nov. (type strain 701T=CGMCC 4.2022T=JCM 13274T), Streptomyces paucisporeus sp. nov. (type strain 1413T=CGMCC 4.2025T=JCM 13276T), Streptomyces rubidus sp. nov. (type strain 13c15T=CGMCC 4.2026T=JCM 13277T) and Streptomyces yanglinensis sp. nov. (type strain 1307T=CGMCC 4.2023T=JCM 13275T); isolates 317 and 913 belong to this latter species.


Assuntos
Microbiologia do Solo , Streptomyces/classificação , Streptomyces/isolamento & purificação , Técnicas de Tipagem Bacteriana , China , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Genes de RNAr , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Filogenia , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Streptomyces/citologia , Streptomyces/fisiologia
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