[Study on the estrogen derivative marked by CdTe quantum dots].

Water-soluble CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) with high quantum yield were synthesized. And the small biomolecule 17beta-amino-estradiol was marked successfully by CdTe QDs. The analysis of UV-Vis spectroscopy, fluorescence spectroscopy, infrared spectroscopy and inverted microscope photographs showed that the surface carboxyl of CdTe quantum dots conjugated with the amino of 17beta-amino-estradiol through the activation of N-hydroxysuccinimide. 17beta-amino-estradiol marked by CdTe QDs successfully provided an experimental basis for new drug screening model.

Highly selective in situ metal ion determination by hybrid electrochemical "adsorption-desorption" and colorimetric methods.

A novel and facile hybrid analytical method coupling electrochemical "adsorption-desorption" and colorimetric analyses was developed to detect heavy metal ions in turbid water samples. The target metal ions were deposited onto an electrode inserted into the original sample, which was referred to as the "adsorption" process. After changing the medium, the concentrated target metal ions were dissolved in a new, clean buffer (blank buffer), which was referred to as the "desorption" process. The concentrations of the target metal ions were measured by colorimetric analyses after the addition of specific indicator amounts. We demonstrated the applicability of this method by detecting Cd(2+), Pb(2+) and Cu(2+) with co-depositing Bi(3+) on portable screen-printed electrodes (SPEs). A good correlation (correlation coefficient of R=0.997) was observed between concentrations ranging from 1 to 200 µM and absorbance values. After the multiple "desorption" process, the even better detection limits as low as 10, 10 and 100 nM were achieved for Cd(2+), Pb(2+) and Cu(2+), respectively. The practicality of this hybrid method was confirmed by the detection of Cd(2+), Pb(2+) and Cu(2+) in wastewater samples, and these results were in agreement with inductively coupled plasma atomic emission spectroscopy (ICP-AES). Overall, this hybrid method provides a simple, selective and effective technique for environmental pollutant analyses.

[Method of antibody analysis based on visual protein array].

AIM: To establish a novel method for antibody analysis based on visual protein microarray. METHODS: The antibodies spotted on the aldehyde-modified slides were incubated with corresponding antigens labeled by biotin, followed by silver enhancement detection method, and the chromogenic images were scanned by CCD camera. RESULTS: The affinity and specificity of each antibody was assayed by this method and six antibodies were found to have higher specificity than the others. A detection limit of 0.4 microg/L was obtained for anti-globulinIIIC013, anti-albumin HPSIIB007, and anti-albumin HPSIIIB007. CONCLUSION: This method is simple, fast and visual. In addition, it needs less sample amount than traditional immunoassay and has potential to achieve high throughput.