Improved microalgae growth and lipid production in anaerobic digestate with ultraviolet radiation pretreatment.

Inappropriate sterilization strategies inhibit microalgal growth when culturing microalgae with anaerobic digestate. This study aimed to scientifically select a low-cost disinfection pretreatment of anaerobic digestate for large-scale microalgae cultivations. In this work, three different methods, including autoclaving, ultraviolet or NaClO treatments, were employed to sterilize the municipal anaerobic digestate. Scenedesmus quadricauda was then cultured in diluted liquid digestate for the simultaneous lipid production and nutrient removal. The results indicated that the growth of S. quadricauda was inhibited after NaClO treatment due to the residual free chlorine. The 15-min ultraviolet effectively mitigated microbial contamination and increasing nutrient availability, enhancing the electron transport of microalgal photosynthesis. After 6-days cultivation, the microalgal biomass concentration of the ultraviolet group was 1.09 g/L, comparable to that of the autoclaving group (1.15 g/L). High nutrient removal efficiency was observed: COD (93.30 %), NH4+-N (92.56 %), TN (85.82 %) and TP (95.12 %). Moreover, S. quadricauda outcompeted the indigenous microorganisms, contributing to its dominance in the culture system of ultraviolet group. The facultative anaerobe Comamonadaceae and aerobes Moraxellaceae, rather than strict anaerobe Paludibacteraceae and Bacteroidetes_vadinHA17, played vital roles in synergistic removal of contaminants by bacteria and algae. The potential competition for nitrogen and phosphorus by bacteria contributed to the ultraviolet group having the greatest lipid content (48.19 %). Therefore, this work suggested using 15-min ultraviolet treatment for anaerobic digestate in large-scale microalgae cultivation.

Formation of autotrophic nitrogen removal granular sludge driven by the dual-partition airlift internal circulation: Insights from performance assessment, community succession, and metabolic mechanism.

Granular sludge has been recognized as an effective method for the application and industrialization of the anammox-based process due to its good biomass retention capacity and environmental tolerance. In this study, a one-stage autotrophic nitrogen removal (ANR) dual-partition system with airlift internal circulation was implemented for 320 days. A high nitrogen removal efficiency of 84.6% was obtained, while the nitrogen removal rate reached 1.28 g-N/L/d. ANR granular sludge dominated by Nitrosomonas and Candidatus Brocadia was successfully cultivated. Results showed that activity and abundance of functional flora first increased with granulation process, but eventually declined slightly when particle size exceeded the optimal range. Total anammox activity was observed to be significantly correlated with protein content (R2 = 0.9623) and nitrogen removal performance (R2 = 0.8796). Correlation network revealed that AnAOB had complex interactions with other bacteria, both synergy for nitrogen removal and competition for substrate. Changes in abundances of genes encoding the Carbohydrate Metabolism, Energy Metabolism, and Membrane Transport suggested energy production and material transfer were possibly blocked with further sludge granulation. Formation of ANR granular sludge promoted the interactions and metabolism of functional microorganisms, and the complex nitrogen metabolic pathways improved the performance stability. These results validated the feasibility of granule formation in the airlift dual-partition system and revealed the response of the ANR system to sludge granulation.

A novel process based on powder carriers demonstrates robustness in nitrogen and phosphorus removal from real municipal wastewater.

The development of efficient and low-consumption wastewater upgrading process is currently at the forefront of the wastewater treatment field. In this study, a novel wastewater treatment process based on powder carriers was proposed. Three systems, namely the activated sludge (AS) system, powder carrier (PC) system, and moving bed biofilm reactor (MBBR) system, were established and operated for over 140 days to treat real municipal wastewater. The characteristics and differences between the three systems were comprehensively investigated. The results suggested that the PC system exhibited notable advantages in nitrogen and phosphorus removal, especially under high influent load and low aeration conditions. The PC system, characterized by a higher nitrification rate compared to the MBBR system and a higher denitrification rate compared to the AS system, contributed to the stable nitrogen removal performance. The particle size of the zoogloea increased under the linkage of the powder carriers, and the mean size of micro-granules reached 170.88 µm. Large number of hydrophobic functional groups on sludge surface, coupled with increased protein content in EPS, further promoted sludge aggregation. Micro-granules formation improved settling performance and enhanced the abundance and activity of functional microbes. A significant enrichment in denitrifying bacteria and denitrifying phosphorus accumulating bacteria was observed in PC system. Up-regulation of the napA, narG, and nosZ genes was responsible for efficient nitrogen removal of the PC system. Moreover, a higher abundance in polyphosphate phosphotransferase (2.11 %) was found in PC system compared with AS and MBBR systems. The increase in the enzymes associated with poly-ß-hydroxybutyrate (PHB) synthesis metabolism in PC system provided the energy for denitrification and phosphorus removal processes.

Enhancement of sewer sediment control and disruption of adhesive gelatinous sediment structure using low-dose calcium peroxide.

Large quantities of sediments in urban sewer systems pose significant risk of pipe clogging and corrosion. Owing to their gel-like structure, sewer sediments have strong resistance to hydraulic shear stress. This study proposed a novel approach to weaken the erosion resistance of sewer sediments by destroying viscous gel-like biopolymers in sediments with low doses of calcium peroxide (CaO2). After treatment with 10-50 mg g-1 TS of CaO2, the critical erosion shear stress was significantly reduced by 25.7%-59.9%. The sediment aggregates gradually disintegrated into small diameter particles with increasing CaO2 dosage. Further analysis showed that the strong oxidizing and alkaline environment induced by CaO2 treatment led to cell lysis and changes in the composition and property of extracellular polymeric substances (EPS). After CaO2 treatment, aromatic proteins and humic acid-like substances associated with adhesion translocated from the inner EPS layers to outer layers while being disintegrated into small organic molecules. Concomitantly, CaO2 treatment disrupted the main functional groups (-OH, COO-, C-N, CO, and CN) in inner EPS layers, thus weakening EPS adhesion. Analysis of protein secondary structure and zeta potential reflected the reduced aggregation capacity of sediment microorganisms and loosening of sediment structure after CaO2 treatment. Thus, CaO2 treatment facilitated fragmentation and disaggregation of the gelatinous structure of sewer sediments. Such green strategy decreased the cost of sewer sediment disposal by 42.10-68.95% when compared to water flushing, and it would improve the self-cleaning capacity of sewer system and efficiency of dredging equipment.

A novel biomineralization-inspired flocculation approach for harvesting high quality microalgal biomass: Dual action of cationic polyelectrolytes and nanosilica.

This study posed a novel biomimetic flocculation approach, aiming to efficiently harvest high-quality biomass of Scenedesmus quadricauda cultured with anaerobic digestate. Here, that poly(diallyldimethylammonium chloride) (PDADMAC) could serve as mimetic silicified proteins to spontaneously incorporate nanosilica onto microalgal cells, imparting diatom-like characteristics to S. quadricauda. Compared to the exponential growth phase (day 3), the highest harvesting efficiency (93.49%) was obtained at a lower dosage of PDADMAC (5 mg/g) in the stationary phase (day 6), which was attributed to changes in properties and composition of microalgal LB-EPS. On day 6, the hydrophobic functional groups in LB-EPS provided more binding sites during the flocculation process and formed a network structure of microalgal cells-flocculants-nanosilica. The resulting larger and more stable biomimetic silica shell promoted microalgal flocculation and sedimentation. Compared to conventional harvesting methods (centrifugation, polyacrylamide, alkaline flocculation), this method had the minimal negative impact on harvested biomass, with 9.95% of cell membranes damaged.

Neglected methane production and toxicity risk in low-frequency ultrasound for controlling harmful algal blooms.

Algal blooms are regarded as a significant source of CH4 emissions. Ultrasound has been gradually employed as a fast and efficient algae removal technology in recent years. However, the changes in water environment and potential ecological effects caused by ultrasonic algae removal are not fully clear. Here, a 40-day microcosm study was performed to simulate the collapse of Microcystis aeruginosa blooms after ultrasonic treatment. The results showed that low-frequency ultrasound at 29.4 kHz for 15 min removed 33.49% of M. aeruginosa and contributed to the destruction of cell structure, but it intensified the leakage of intracellular algal organic matter and microcystins. The accelerated collapse of M. aeruginosa blooms after ultrasonication promoted the rapid formation of anaerobic and reductive methanogenesis conditions, and elevated dissolved organic carbon content. Moreover, the release of labile organics, including tyrosine, tryptophan, protein-like compositions, and aromatic proteins, was facilitated by the collapse of M. aeruginosa blooms after ultrasonic treatment, and they supported the growth of anaerobic fermentation bacteria and hydrogenotrophic Methanobacteriales. This was also demonstrated by the increase in methyl-coenzyme M reductase (mcrA) genes in sonicated algae added treatments at the end of incubation. Finally, the CH4 production in sonicated algae added treatments was 1.43-fold higher than that in non-sonicated algae added treatments. These observations suggested that ultrasound for algal bloom control potentially increased the toxicity of treated water and its greenhouse gas emissions. This study can provide new insights and guidance to evaluate environmental effects of ultrasonic algae removal.

Enhanced growth and auto-flocculation of Scenedesmus quadricauda in anaerobic digestate using high light intensity and nanosilica: A biomineralization-inspired strategy.

Coupling municipal anaerobic digestate (MAD) treatments with microalgal cultivation can concomitantly achieve nutrient removal and microalgal bioenergy production. However, the high cost caused by dilution water and microalgal harvesting is a great challenge. In this study, Scenedesmus quadricauda was screened as the most appropriate algae strain due to its potential for growth and auto-flocculation, and the MAD diluted 5-fold with WWTP effluent was demonstrated as an ideal medium for S. quadricauda growth. Moreover, inspired by naturally generated silica shells of diatoms, a low-cost and biomimetic auto-flocculation strategy that combined high light intensity induction and microalgal silicification was proposed to accelerate the auto-flocculation process. Compared with low light intensity groups, this strategy imparted diatom-like features to S. quadricauda cells, and contributed to 3.07-fold higher auto-flocculation efficiency within 30 min. It was attributed to the fact that the high light intensity of 150 µmol·m - 2·s - 1 stimulated the extracellular polymeric substances (EPS) secretion and induced the variation in property and composition of EPS, especially the protein secondary structures, which allowed silica nanoparticles to spontaneously attach onto S. quadricauda cells in the presence of viscous EPS. Furthermore, this strategy significantly increased microalgal biomass yield to a dry weight of 1.37 g·L - 1, accompanied by 93.78%, 96.39% and 91.36% removals of NH4+-N, TP, and COD, respectively. The productivity of valuable by-products, including lipid, carbohydrate, protein, and pigment, reached 56.30, 101.35, 30.39 and 11.28 mg·L - 1·d - 1, respectively. Overall, this study supplies a novel approach for low-cost microalgal bioenergy production from MAD and energy-efficient microalgae harvest by auto-flocculation.

Addressing algal blooms by bio-pumps to reduce greenhouse gas production and emissions with multi-path.

The collapse of dense algal blooms is identified as a significant source of methane (CH4) emissions. When flocculation is used for algae removal, algal carbon is often turned into CH4 and carbon dioxide (CO2). Here, we established a "bio-pump" to control algal blooms and reduce greenhouse gas (GHG) emissions by the introduction of submerged macrophytes to the aquatic ecosystem and combination of flocculation and capping. The results suggested that this strategy contributed to an approximately 98% algae removal and sustainably improved dissolved oxygen (DO) in the water and sediment after the 40-day incubation. The aerobic condition at the sediment-water interface and deeper oxygen penetration in the sediment inhibited the abundance of microorganisms related to anaerobic CH4 production, then changed the metabolic pathway and fate of algal carbon. After the 40-day incubation, compared with flocculation-capping treatments, the bio-pump reduced 69.07% CH4 and 77.57% CO2 emissions, which was jointly contributed by the inhibition of anaerobic CH4 production, aerobic oxidation of CH4 and carbon sequestration of submerged macrophytes. This was also demonstrated from the finding of a decrease in methyl coenzyme M reductase (mcrA) gene, an increase in particulate methane monooxygenase (pmoA) gene and the absorption of 13C-labeled from algae biomass by submerged macrophytes at the end of incubation. Therefore, the bio-pump established in the present study can improve DO in algal blooms water and turn algal-derived organic matter into the plant biomass, which supplied a sustainable method for algae removal and GHG reduction.

Underestimated methane production triggered by phytoplankton succession in river-reservoir systems: Evidence from a microcosm study.

The impoundment of dammed rivers accelerates phytoplankton succession from river-dominated to lake-dominated species. Little is known about the role of phytoplankton succession in methane (CH4) production. In this study, we performed a 61-day microcosm investigation to simulate the collapse processes of Cyclotella meneghiniana (river-dominated algae) and Chlorella pyrenoidosa and Microcystis aeruginosa (lake-dominated algae). The results suggested that different methanogenic conditions were induced by the collapse of river-and lake-dominated algae. The rapid settlement of C. meneghiniana induced aerobic conditions in the water that inhibited anaerobic CH4 production and intensified CH4 oxidation as a result of an increase in pmoA. However, the decomposition of C. pyrenoidosa and M. aeruginosa depleted dissolved oxygen and provided abundant labile organic matter, which jointly elevated mcrA and the mcrA/pmoA ratio. Under this condition, anaerobic CH4 production was the dominant pathway for the mineralization of algae-derived carbon. Finally, the CH4 produced per unit of particulate total carbon (identified as the carbon content of the algal biomass) by C. pyrenoidosa and M. aeruginosa was 16.29-fold and 8.56-fold higher, respectively, than that produced by C. meneghiniana. These observations provided evidence that lake-dominated algae played a more vital role in CH4 production than river-dominated algae when algal succession occurred. This discovery might be a new and vital, yet largely underestimated CH4 emission pathway in river-reservoir systems, that should be considered when evaluating the effect of hydraulic projects on greenhouse gas emissions.