Interactions of the Cyanobacterium Chrysosporum ovalisporum with Antibiotics in Water.

In this study, we assessed the effects of 11-day exposure of sulfadiazine (SD), sulfamethazine (SM2), norfloxacin (NOR), and enrofloxacin (ENR) on the growth, chlorophyll a (Chl. a) content, phycobiliproteins (PBPs) content, and alkaline phosphatase (ALP) activity of Chrysosporum ovalisporum, examined the removal rate of these antibiotics by C. ovalisporum, and performed acute toxicology test with Daphnia magna to determine the effect of interaction between antibiotics and cyanobacteria on aquatic animals. The results showed that the stress of SD and SM2 increased extracellular ALP activity and weakly inhibited the algal growth and the contents of Chl. a and PBPs compared with that noted in the control. ENR and NOR treatment groups exerted significant inhibition on algal growth as well as Chl. a and PBPs contents and ALP activity, although the cyanobacterium could degrade these two antibiotics more than SD and SM2. The results also revealed that the interaction between antibiotics and cyanobacteria could inhibit D. magna feeding.

Biochemical responses of the freshwater microalga Dictyosphaerium sp. upon exposure to three sulfonamides.

Sulfonamides (SAs) are common antimicrobial drugs, which are frequently detected in surface water systems, and are difficult to degrade, posing a potential threat to the aquatic environment. However, little is known about the potential adverse effects of SAs on non-target organisms (e.g., microalgae) in the aquatic ecosystem. In this study, the effect of SAs (sulfadiazine (SD), sulfamerazine (SM1), and sulfamethazine (SM2) at 1, 5, 20, and 50 mg/L concentrations, respectively) on the freshwater microalga Dictyosphaerium sp. was investigated, with respect to changes of biomass and chlorophyll a content and induction of extracellular polymer substances (EPS), including protein and polysaccharide contents. At the same time, the residue of SAs was determined. The results showed that Dictyosphaerium sp. was tolerant to the three SAs, and the chlorophyll a content in Dictyosphaerium sp. significantly decreased on day 7, followed by a "compensation phenomena". The increase in protein and polysaccharide contents played a defensive role in Dictyosphaerium sp. against antibiotic stress, and there was a strong positive correlation between polysaccharide contents and antibiotic concentrations. Dictyosphaerium sp. exhibited 35%-45%, 30%-42%, and 26%-51% removal of SD, SM1, and SM2, respectively. This study is helpful to understand the changes of EPS in the defense process of microalgae under the action of antibiotics, and provides a new insight for the ecological removal of antibiotic pollution in natural surface water system.

Catalytic degradation mechanism of sulfamethazine via photosynergy of monoclinic BiVO4and microalgae under visible-light irradiation.

To improve the efficiency of antibiotic degradation, the photosynergistic performance of bismuth vanadate (BiVO4) with a microalga, Dictyosphaerium sp., was demonstrated under visible-light irradiation for the first time. Sulfamethazine (SM2) was selected as a representative sulfanilamide antibiotic, and the photocatalytic degradation mechanism of SM2 was evaluated in media via the BiVO4-algae system. The hydrothermally synthesized sample was characterized using X-ray powder diffraction, X-ray photoelectron spectroscopy, ultraviolet-visible diffuse reflectance spectroscopy, transmission electron microscopy, Brunauer-Emmett-Teller surface area, and Fourier transform infrared spectroscopy techniques. The results demonstrated that the prepared photocatalyst corresponded to phase-pure monoclinic scheelite BiVO4. The synthesized BiVO4 showed superior photocatalytic properties under irradiation with visible light, and more than 80% of photocatalytic degradation efficiency was obtained by the BiVO4-algae system. Based on quenching experiments, the photocatalytic degradation of SM2 in the BiVO4-algae system was primarily accomplished via the generation of triplet state dissolved organic matter, and hydroxyl radicals played a small role in the degradation process. The direct oxidation of holes made no contribution to the degradation. Metabolomics data showed that a total of 91 metabolites were significantly changed between the two comparison groups (algae-SM2 group vs algae group; algae-BiVO4-SM2 group vs algae-BiVO4 group). The glycometabolism pathways were increased and the tricarboxylic acid cycle was activated when BiVO4 was present. The study provides a distinctive approach to remove antibiotics using visible light in the aqueous environment.

Sulfonamides-induced oxidative stress in freshwater microalga Chlorella vulgaris: Evaluation of growth, photosynthesis, antioxidants, ultrastructure, and nucleic acids.

Sulfadiazine (SD), sulfamerazine (SM1), and sulfamethazine (SM2) are widely used and disorderly discharged into surface water, causing contamination of lakes and rivers. However, microalgae are regard as a potential resource to alleviate and degrade antibiotic pollution. The physiological changes of Chlorella vulgaris in the presence of three sulfonamides (SAs) with varying numbers of -CH3 groups and its SA-removal efficiency were investigated following a 7-day exposure experiment. Our results showed that the growth inhibitory effect of SD (7.9-22.6%), SM1 (7.2-45.9%), and SM2 (10.3-44%) resulted in increased proteins and decreased soluble sugars. Oxidative stress caused an increase in superoxide dismutase and glutathione reductase levels but decreased catalase level. The antioxidant responses were insufficient to cope-up with reactive oxygen species (hydrogen peroxide and superoxide anion) levels and prevent oxidative damage (malondialdehyde level). The ultrastructure and DNA of SA-treated algal cells were affected, as evident from the considerable changes in the cell wall, chloroplast, and mitochondrion, and DNA migration. C. vulgaris-mediated was able to remove up to 29% of SD, 16% of SM1, and 15% of SM2. Our results suggest that certain concentrations of specific antibiotics may induce algal growth, and algal-mediated biodegradation process can accelerate the removal of antibiotic contamination.

Ecotoxicological effects of sulfonamides and fluoroquinolones and their removal by a green alga (Chlorella vulgaris) and a cyanobacterium (Chrysosporum ovalisporum).

In recent years, antibiotic pollution has become worse, especially in China. In this study, the ecotoxicological effects of four frequently used antibiotics with different lipophilic degrees (log Kow) (sulfadiazine (SD), sulfamethazine (SM2), enrofloxacin (ENR), and norfloxacin (NOR)) at four concentrations of 1, 5, 20, and 50 mg L-1 were examined using batch cultures of green alga Chlorella vulgaris and cyanobacterium Chrysosporum ovalisporum for 16 days based on changes in chlorophyll fluorescence parameters (chl a, Fv/Fm, and ΦPSII) and responses of the antioxidant system. Besides, the antibiotics removal efficiencies of the two microalgae were investigated. Sulfonamides (SD and SM2) had no significant inhibitory effect on the growth of C. ovalisporum, but had an inhibitory effect on C. vulgaris, whereas fluoroquinolones (ENR and NOR) significantly inhibited C. ovalisporum. The activities of superoxide dismutase, catalase, and glutathione reductase suggested that C. vulgaris was more tolerant to these antibiotics than C. ovalisporum. The increased malondialdehyde level in both algae indicated their tolerance against antibiotics. When compared with C. ovalisporum, C. vulgaris presented better capacity to remove antibiotics. In summary, the four antibiotics exerted time- or concentration-dependent ecotoxicological effects on the microalgae examined, whereas the microalgae could remove the antibiotics based on the log Kow of the antibiotics. The findings of this study contribute to effective understanding of the ecotoxicological effects of antibiotics and their removal by microalgae.

Ecotoxicological effects of sulfonamide on and its removal by the submerged plant Vallisneria natans (Lour.) Hara.

The extensive application of sulfonamides (SAs) raises concern regarding its negative environmental effects. In aquatic environments, macrophytes may not only be affected by various pollutants, they may also help to reduce the concentrations in the surrounding environment. We studied both the ecotoxicological effects of sulfonamide (SN) on and its removal by Vallisneria natans (Lour.) Hara, an important submerged macrophyte in Chinese lakes and rivers. The toxic effect and oxidative stress caused by SN resulted in a reduction of total chlorophyll (chl.a and b) and autofluorescence of chloroplast. Meanwhile, the levels of reactive oxygen species (ROS, including O2- and H2O2) and peroxidase (POD) increased with increasing SN concentration and duration of exposure. After 20 days' exposure, a reduction in the relative growth rate (RGR) and leaf length of V. natans was found under SN stress, but SN had only a weak effect on root length. Although high SN concentrations had toxic effects on the growth of V. natans, the plant was overall resistant to the SN doses that we used. We studied the effect of V. natans on sulfonamide removal in an additional 13-day exposure experiment with focus on the dynamics of dissolved oxygen (DO), the oxidation-reduction potential (ORP) and microbial communities in the water column, as well as in the periphyton on V. natans surfaces. The results show that presence of V. natans significantly improved the SN removal efficiency likely by increasing DO, ORP and bacterial diversity in the water column. The presence of V. natans led to higher relative abundances of Saccharimonadales and Rhizoniales. Lefse analysis showed that Saccharimonadales, Micrococcales, Sphingobacteriales, Bacteroidales, Obscuribacterales, Flavobacteriales, Pseudomonadaceae and Myxococcales, which are considered to be SN-resistant bacteria, increased significantly in the V + S+ (V. natans and SN) treatment compared with the V + S- (V. natans and no SN) treatment and V-S+ (no V. natans and SN) treatment. As far as we know, ours is the first study of the ecotoxicological effects of sulfonamide and its removal by submerged vascular plants (here V. natans). Thus, our results add to the understanding of the antibiotic removal mechanism of macrophytes in freshwater systems and help to clarify the linkages between antibiotics and macrophyte-microbe systems; thereby providing new insight into ecological-based removal of antibiotics in aquatic systems.