RESUMO
Sodium butyrate (SB) is a short chain 4-carbon fatty acid salt naturally exists in animal fats. Previous studies have proven that sodium butyrate has many beneficial functions such as anti-tumor and anti-inflammatory actions. In the current study we investigated the effect and possible mechanism of sodium butyrate in LPS-induced acute lung injury (ALI). ALI was induced by intratracheal administration of LPS (10â¯mg/kg) in male BALB/c mice. Sodium butyrate (500â¯mg/kg) was administered intraperitoneally 30â¯min prior to LPS exposure. We found that sodium butyrate significantly protected animals from LPS-induced ALI as evidenced by decreased the lung wet to dry weight ratio, total cells, neutrophils, macrophages, myeloperoxidase (MPO) activity, and lung histological damage compared to vehicle control. Sodium butyrate pretreatment markedly inhibited the production of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Furthermore, sodium butyrate pretreatment dramatically suppressed HMGB1 release and NF-κ B activation. Together, these results suggest that sodium butyrate pretreatment protects mice from LPS-induced acute lung injury, possibly through the modulation of HMGB1 and inflammatory responses.
Assuntos
Lesão Pulmonar Aguda/tratamento farmacológico , Anti-Inflamatórios/uso terapêutico , Ácido Butírico/uso terapêutico , Proteína HMGB1/metabolismo , Pulmão/metabolismo , Macrófagos/imunologia , Neutrófilos/imunologia , Animais , Citocinas/metabolismo , Humanos , Mediadores da Inflamação/metabolismo , Injeções Intraperitoneais , Lipopolissacarídeos/imunologia , Pulmão/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , NF-kappa B/metabolismo , Peroxidase/metabolismoAssuntos
Evolução Molecular , Genoma de Planta , Ginsenosídeos/biossíntese , Panax notoginseng/genética , Plantas Medicinais/genética , Sequência de Bases , Vias Biossintéticas/genética , Regulação da Expressão Gênica de Plantas , Ginsenosídeos/química , Família Multigênica , Metabolismo Secundário/genéticaRESUMO
Trident maple Acer buergerianum Miq., belonging to the family of Aceraceae, is an important ornamental tree. Here, we report the complete chloroplast genome sequence of A. buergerianum. The circular genome was 156,477 bp in size, and comprised a pair of inverted repeat (IR) regions of 26,090 bp, a large single-copy (LSC) region of 86,246 bp and a small single-copy (SSC) region of 18,062 bp. It contained 134 genes, including 89 protein-coding genes, 40 transfer RNA genes, and 8 ribosomal genes. Maximum likelihood phylogenomic analysis shows that A. buergerianum is closely related to other Acer species, including A. miaotaiense, A. morrisonense, and A. davidii.
