RESUMO
The use of fungicides to control plant diseases creates a potential health risk. One alternative to this problem is the biological control, which has been succesfully applied to control plant diseases. Bacillus atrophaeus HAB-5 exhibits a high inhibitory acitivities against different fungal pathogens and suppresses them. The aim of current studies is to produce and identify the antifungal compounds produced by the strain HAB-5. We found that the submerge fermentation harvested from Luria-Bertani (LB) medium had the highest activity against Colletotrichum gloeosporioides. The petroleum ether crude extract was strongly bioactive and its activity was stable after heat treatment, pH treatment, illuminated light as well as ultra violet exposition. The antifungal compounds were purified using gel chromatography column. Based on Gas Chromatography-Mass Spectrometry (GC-MS) analysis, nineteen different volatile organic compounds (VOCs) were identified included the range of alkanes, alkenes, alcohols, and organics acid. Among these identified compounds, Chloroacetic acid, tetradecyl esters followed by Octadecane and Hexadecanoic acid, methyl ester showed antifungal activity against C. gloeosporioides. Our results clearly showed Chloroacetic acid, tetradecyl esters; Octadecane and Hexadecanoic acid, methyl ester are key inhibitory compounds produced by Bacillus atrophaeus HAB-5 against C. gloeosporioides.
Assuntos
Antifúngicos/química , Antifúngicos/farmacologia , Bacillus/química , Colletotrichum/efeitos dos fármacos , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/farmacologia , Fusarium/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Doenças das Plantas/microbiologiaRESUMO
The roles of most MYB transcription factors (TFs) in the poplar remain unclear. Here, we demonstrate that PtrSSR1, a salt-stress-regulator in the Populus trichocarpa R2R3 MYB gene family, mediates the tolerance of transgenic Arabidopsis plants to salt stress. The transcripts of PtrSSR1 could be induced by salt stress rapidly in poplar. Subcellular localization and yeast assays indicated that PtrSSR1 encoded a nuclear protein with transactivation activity. The Arabidopsis transformants overexpressing PtrSSR1 clearly displayed lateral root emergence (LRE) inhibition compared with wild-type (Wt) under normal conditions; while upon NaCl treatment, the transformants showed improved tolerance, and the LRs emerged faster from salt-induced inhibition. A strong correlation could exist between the LRE mediated by PtrSSR1 and abscisic acid (ABA), mainly because the transformants displayed more sensitivity to exogenous ABA during both seed germination and LRE, and had a distinctly increased level of endogenous ABA. Furthermore, several ABA- and salt-related genes, such as NCED3, ABI1 and CBL1, were up-regulated. Thus, our results suggest that elevation in the endogenous ABA content bring alteration of plant LR development, and that the poplar R2R3 MYB TF PtrSSR1 vitally improve salt stress tolerance by integrating the regulation of LRE and ABA signaling in Arabidopsis.