RESUMO
Bone-like GdF3 was synthesized and applied for phosphopeptide enrichment for the first time. As a new kind of efficient phosphopeptide affinity probe, GdF3 exhibits high efficiency in the mediation of the dephosphorylation reaction. In addition, DFT calculations were introduced to theoretically explain the unique property of GdF3 compared to GdPO4, which is promising and can be potentially significant in protein phosphorylation research.
Assuntos
Fluoretos/química , Gadolínio/química , Fosfopeptídeos/química , Marcadores de Afinidade , Fluoretos/síntese química , Modelos Moleculares , Nanoestruturas/química , Fosfopeptídeos/análise , Fosforilação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
Guanidyl-functionalized graphene (GFG) was designed and synthesized. By simple modulation of the loading buffer this novel bifunctional adsorbent provides two enriching functions, one for global phosphopeptides, and the other for multiphosphopeptides with consecutive phosphorylated residues. GFG provides a promising perspective for consecutive phosphorylated peptide enrichment in phosphor-proteomic research and related biological process studies.
Assuntos
Grafite/química , Guanina/química , Fosfopeptídeos/análise , Proteômica , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Caseínas/metabolismo , Cromatografia Líquida de Alta Pressão , Nanopartículas/química , Peptídeos/química , Fosfopeptídeos/isolamento & purificação , Tripsina/metabolismoRESUMO
ZnSn(OH)(6) and binary-component SnO(2)-ZnSn(OH)(6) were introduced as affinity probes for phosphopeptide enrichment for the first time. Two strategies, either ZnSn(OH)(6) and SnO(2) serial enrichment or binary-component SnO(2)-ZnSn(OH)(6) enrichment in a single run, were proposed to enhance multi-phosphopeptide enrichment and to significantly improve global phosphopeptide detection.
