Bioaccessibility of phospholipids in homogenized goat milk: Lipid digestion ecology through INFOGEST model.

Phospholipids-rich goat milk provides health benefits to consumers. The effects of homogenization on the disruption and recombination of milk fat globule membrane and change the fatty acid positional distribution in glycerophospholipids profile by phosphatidylcholine metabolism pathways were investigated. Goat milk was homogenized at different intensity pressure. Homogenized samples were introduced into harmonized INFOGEST digestion model. Results showed that phosphatidylcholine increased significantly during storage in 30 MPa and were approximately twice that in raw milk (LOD 0.27-1.49 µg/L and LOQ 0.89-4.92 µg/L, respectively). Meanwhile, both linoleic acid (C18:2) and α-linolenic acid (C18:3ω-3), the foremost polyunsaturated acyl chains in homogenized milk extracts, showed upward trends. Notably, homogenization increased the number and altered the composition of Sn-1, 2 diacylglycerols via increasing trypsin and pancreatic lipase (PLRP2, MAUC15, CD36 and BSSL) expression and accelerated the phosphatidylcholine conversion. Ultimately, the relationship between homogenization and milk fat globule recombination and phospholipids bioaccessibility was preliminary established.

Proteomics analysis to investigate the impact of diversified thermal processing on meat tenderness in Hengshan goat meat.

During the thermal processing, proteins of Hengshan goat meat undergo structural modifications such as degradation, oxidation and denaturation, ultimately affect the palatability and acceptability. The results of several objective metrics demonstrated that thermal processing exhibited significant impacts on the tenderness of goat meat. The 551, 84, 72, and 121 proteins were identified in the control and thermal processed groups (boiled, steamed, and roasted), respectively. Compared with the control group, the 101, 98, and 109 differentially-expressed proteins were explored in the treatment groups. Furthermore, the functions of metabolic and skeletal muscle proteome were investigated and discussed. Sensory evaluation and proteomics analysis showed that steaming and boiling treatment had no significant effect on the tenderness of goat meat, while roasting significantly reduced the tenderness, indicating that the available thermal processing methods to ensure the tenderness of goat meat were steaming and boiling treatments. Thus, the established proteomics database of goat meat provided the valuable reference for rational selection of thermal processing methods.

Novel strategy to remove the odor in goat milk: Dynamic discovey magnetic field treatment to reduce the loss of phosphatidylcholine in flash vacuum from the proteomics perspective.

In present study, a precisely profile of PC species in goat milk was presented by quantitative lipidomics, and the matrix effect (bovine, goat and breast milk) on the lipase catalysis of PC metabolism patterns was explored via proteomics. The effects of flash vacuum and magnetic field processes to PC profile were investigated. Results showed PC(16:0_18:1) (1365.24 µg/mL) and PC(16:0_20:2) (1354.73 µg/mL) had the most abundant intensity in goat milk. Twelve novel bioactive lipases: LDHB, NSDHL, ALDH3B1, DPYD, ALDH1A1, ALDOC, ENO1, ALDOA, PRDX6, XDH, ENO3 and GAPDH were nuclear-localized in PC biosynthesis. PC in C15:0, C16:0 increased while C6:0, C8:0 decreased and the characterized protein XDH was about 91 times up regulated under 0.085 MPa, 65 °C flash vacuum and 5 mT magnetic field. The findings suggest different bioactive lipases show desirable effects on PC species metabolism, and magnetic field realize a beneficial programming impact on reducing the loss of PC.

Accurate Quantification of Sulfonamide Metabolites in Goat Meat: A New Strategy for Minimizing Interaction between Sheep Serum Albumin and Sulfonamide Metabolites.

To date, the determination of sulfonamide metabolites in animal-derived food has universal disadvantages of low throughput and no integrated metabolites involved. In this study, a powerful and reliable strategy for high-throughput screening of sulfonamide metabolites in goat meat was proposed based on an aqueous two-phase separation procedure (ATPS) combined with ultrahigh-performance liquid chromatography quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap). Noncovalent interactions including van der Waals force, hydrogen bonding, and hydrophobic effect were determined to be staple interactions between the sulfonamide metabolites and sheep serum albumin by fluorescence spectroscopy and molecular docking technology, and an 80% acetonitrile-water solution/(NH4)2SO4 was used as ATPS in order to release combined sulfonamide metabolites and minimize the influence of sheep serum albumin. Sulfonamide metabolites in the matrix were screened based on a mechanism of mass natural loss and core structure followed by identification combined with the pharmacokinetic. The developed strategy was validated according to EU standard 2002/657/EC with CCα ranging from 0.07 to 0.98 µg kg-1, accuracy recovery with 84-107%, and RSDs lower than 8.9%. Eighty seven goat meat samples were used for determination of 26 sulfonamides and 8 potential metabolites. On the basis of the established innovative process, this study has successfully implemented the comprehensive detection of sulfonamide metabolites, including N4-acetylated substitution, N4-hydroxylation, 4-nitroso, azo dimers, oxidized nitro, N4 monoglucose conjugation, ß-d-glucuronide, and N-4-aminobenzenesulfonyl metabolites, which were shown to undergo oxidation, hydrogenation, sulfation, glucuronidation, glucosylation, and O-aminomethylation.

Molecular mechanism of protein dynamic change for Hengshan goat meat during freezing storage based on high-throughput proteomics.

As traditional frozen storage leads to the degradation of meat quality, elucidation of dynamic change mechanism is urgently needed. Proteomic differences in postmortem frozen storage time (0, 30 and 60 days) of Hengshan goat meat at -18 °C were studied by label-free proteomics based on high resolution quadrupole-Orbitrap mass spectrometry. A total of 492 proteins were identified, of which 485 proteins were quantified, and the difference of 199 proteins was observed. The analysis of the differentially expressed proteins related to quality observed that triosephosphate isomerase and peroxiredoxin-6 were potential biomarkers for goat meat discoloration. Troponin and myosin can represent the tenderness of goat meat. Heat shock protein 70 can be used as water-retaining proteins in goat meat. Bioinformatics analysis suggested that the distinguishingly expressed proteins were involved in glycolysis and the ubiquitin-proteasome pathway revealing that the strong degradation of proteins, which cause of the degradation of long-term frozen meat quality. These results could enrich and beyond the existing knowledge of frozen meat, expecting to have a further understanding of the changes of meat quality at the molecular level.