Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Assunto principal
Intervalo de ano de publicação
1.
FEMS Microbiol Lett ; 317(2): 160-71, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21261700

RESUMO

Bacterial swarming motility is a flagella-dependent translocation on the surface environment. It has received extensive attention as a population behavior involving numerous genes. Here, we report that Citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriate agar concentration. The swarming behavior of C. freundii was described in detail. Insertional mutagenesis with transposon Mini-Tn5 was carried out to discover genetic determinants related to the swarming of C. freundii. A number of swarming genes were identified, among which flhD, motA, motB, wzx, rfaL, rfaJ, rfbX, rfaG, rcsD, rcsC, gshB, fabF, dam, pgi, and rssB have been characterized previously in other species. In mutants related to lipopolysaccharide synthesis and RcsCDB signal system, a propensity to form poorly motile bacterial aggregates on the agar surface was observed. The aggregates hampered bacterial surface migration. In several mutants, the insertion sites were identified to be in the ORF of yqhC, yeeZ, CKO_03941, glgC, and ttrA, which have never been shown to be involved in swarming. Our results revealed several novel characteristics of swarming motility in C. freundii which are worthy of further study.


Assuntos
Citrobacter freundii/fisiologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Western Blotting , Citrobacter freundii/genética , Citrobacter freundii/ultraestrutura , Microscopia Eletrônica de Transmissão , Fases de Leitura Aberta/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...