Transcriptomics Analysis of the Immune Effects of Okadaic Acid on Caco-2 Cells.

Okadaic Acid, a type of diarrhetic shellfish poison, is widely distributed and harmful, causing symptoms such as diarrhea, vomiting, and more in humans. Recent studies have demonstrated that OA can lead to various toxicities such as cytotoxicity, neurotoxicity, embryotoxicity, and hepatotoxicity. In order to investigate the immunotoxicity of OA on intestinal cells, a transcriptome analysis was conducted to compare the differences in the Caco-2 cell transcriptional group before and after administration. The CCK-8 experiment demonstrated that OA had a detrimental effect on the activity of Caco-2 cells, with an IC50 value of 33.98 nM. Transcriptome data revealed changes in immune-related genes between the experimental and control groups, including inflammatory factors, heat shock proteins, and zinc finger proteins. The analysis of the results suggests that OA can induce the production of inflammatory factors and apoptosis in cells, and may also affect cell ferroptosis. These findings indicate that OA has a significant impact on intestinal immunity, providing valuable insights for the study of immune toxicity associated with OA.

Combined dynamic transcriptomics and metabolomics analyses revealed the effects of trans-vp28 gene Synechocystis sp. PCC6803 on the hepatopancreas of Litopenaeus vannamei.

Litopenaeus vannamei is the most important shrimp species throughout the world. However, diseases are increasing with the development of the industry, so enhancing the immunity of shrimp is of great significance. In this study, 1800 shrimp were divided into two groups randomly: the control group (N, feed with brine shrimp flake) and the experimental group (M, feed with mutant of Synechocystis sp. cells) (300 shrimp/group/replication) and each trial was conducted in triplicates. After immunization, sixty shrimp (with three replicates of twenty) were collected at 0 h in group N and 24, 72, and 144 h in group M, respectively, and the hepatopancreas were isolated for transcriptomic and metabolomic analysis. Transcriptome data revealed that compared with group N, genes related to antimicrobial peptides, cytoskeleton remodeling, detoxification, apoptosis, blood coagulation, immune defense, and antioxidant systems were differentially expressed in group M. In addition, combined transcriptomic and metabolomic analysis revealed that some immune-related differential genes or differential metabolites were consistently expressed in both omics. All the above results indicated that trans-vp28 gene Synechocystis sp. PCC6803 could improve the immunity of L. vannamei. This is the first report of the integration of dynamic transcriptomics combined with metabolomics to study the effect of trans-vp28 gene Synechocystis sp. PCC6803 in the hepatopancreas of L. vannamei and provided important information about the defense and immune mechanisms used by invertebrates against pathogens.

A proteomics investigation of 'immune priming' in Penaeus vannamei as shown by isobaric tags for relative and absolute quantification.

Invertebrates are considered completely dependent on their innate immunity to defend themselves against pathogens as they lack an adaptive immunity. However, a growing body of evidence has indicated a specific acquired immunity called 'immune priming' may exist. The Pacific white shrimp, Penaeus vannamei is one of the most economically important shrimp species in the world. In the previous research, we investigated the hepatopancreas immune response of shrimp immunized with trans -vp28 gene Synechocystis sp. PCC6803 at the protein level. In this study, on the basis of the previous research, the shrimp were then challenged with WSSV, and hepatopancreas analyzed using isobaric tags for relative and absolute quantification (i TRAQ) labeling. In total, 308 differentially expressed proteins (DEPs) were identified including 84 upregulated and 224 downregulated. Upregulated proteins such as calmodulin B and calreticulin, and downregulated proteins such as calnexin, and signaling pathways like Ras, mTOR were differentially expressed in both studies. Data from this study are more significant than previous work and indicate increased sensitivity to WSSV after immunization with trans-vp28 gene Synechocystis sp. PCC6803. In addition, selected DEPs (upregulated: A0A3R7QHH6 and downregulated: A0A3R7PEF6, A0A3R7MGX8, A0A423TPJ4, and A0A3R7QCC2) were randomly analyzed using parallel reaction monitoring (PRM). These data preliminarily confirm immune priming in P. vannamei, and show that the initial stimulation with trans -vp28 gene Synechocystis sp. PCC6803 regulate P. vannamei immune responses and they provide shrimp with enhanced immune protection against secondary stimulation.

Susceptibility of five different sizes of pathogenfree Litopenaeus vannamei to white spot syndrome virus (WSSV) by intramuscular inoculation.

White spot syndrome virus (WWSV) has become one of the most widespread causes of mortality in commercial shrimp farming. In the present study, we used PCR to determine the shrimp infectious dose 50% endpoint (SID50 ml-1) of a Chinese isolate of WSSV in 5 different sizes of pathogen-free Litopenaeus vannamei inoculated intramuscularly. The lethal dose 50% endpoint (LD50 ml-1) was also determined from the percentage of dead shrimp. The LD50 ml-1 for 2, 4, 6, 8, and 10 cm shrimp were 104.68, 105.7, 106.70, 107.75, and 108.81, respectively, and the SID50 ml-1 were 104.68, 105.70, 106.90, 107.75, and 108.94, respectively. There was no significant difference between the LD50 ml-1 and SID50 ml-1 for each shrimp size, which indicated that all infected shrimp died. The lethal and infectious titer decreased about 1 log10 as shrimp size decreased 1 grade. These data clearly indicate that adult shrimp were more susceptible to WSSV than juvenile shrimp. The horizontal comparison showed that the amount of virus in the shrimp organs increased over the experimental period. The vertical comparison showed that virus quantity was lowest in the organs of 10 cm shrimp and highest in 2 cm shrimp, which indicates that the smaller shrimp had higher levels of viral replication. Hence, the optimal size for WSSV challenge in shrimp inoculated intramuscularly was 2 cm. The determination of virus titers in different sizes of shrimp represents a step towards creating strategies to reduce the negative impacts of WSSV in the aquaculture industry.